Human ANGII EIA

$376.00
EIA-ANGII-1

RayBio® Human/Mouse/Rat Angiotensin II EIA Kit optimized for serum. Competition-based ELISA on a 96-well strip plate.

Introduction

Angiotensin, a key player in the renin-angiotensin system, is a peptide hormone that causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenal cortex. It is derived from the precursor molecule angiotensinogen produced in the liver.

Angiotensin II is formed from Angiotensin I, which is removed of two terminal residues by the enzyme Angiotensin-converting enzyme (ACE). Angiotensin II acts as an endocrine, autocrine/ paracrine, and intracrine hormone. Angiotensin II is degraded to angiotensin III by angiotensinases that are located in red blood cells and the vascular beds of most tissues. It has a half-life in circulation of around 30 seconds, while in tissue, it may be as long as 15-30 minutes.

The effect of obesity on Angiotensin II has recently been reported. Obese patients show heightened renal vasodilation to blockade of the renin-angiotensin system, suggesting deficits in vascular responses to angiotensin II. This may due to increased reactivity of renal vasoconstriction to ANG II.

Angiotensin II has been associated with a number of important physiological processes in heart, brain, adrenal gland and kidney. For cardiovascular effect, Angiotensin II is a potent direct vasoconstrictor, constricting arteries and veins and increasing blood pressure. It is also the most important Gq stimulator of the heart during hypertrophy. For neural effects, Angiotensin II increases thirst sensation (dipsogen) through the subfornical organ (SFO) of the brain, decreases the response of the baroreceptor reflex, and increases the desire for salt. It increases secretion of ADH in the posterior pituitary and secretion of ACTH in the anterior pituitary. For adrenal effects, Angiotensin II acts on the adrenal cortex, causing it to release aldosterone. For renal effects, Angiotensin II has a direct effect on the proximal tubules to increase Na+ absorption.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

The capture antibody provided in this kit recognizes human, mouse and rat Angiotensin II.

Target Protein Information

Synonyms
Angiotensinogen (Serpin A8) [Cleaved into: Angiotensin II (Ang II)]
Accession Number
Gene Names
AGT SERPINA8
Gene ID
183

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
  • Prepare all reagents, samples and standards as instructed.
  • Add 100 µl detection antibody to each well.
  • Incubate 1.5 h at RT or O/N at 4°C.
  • Add 100 µl standard or sample to each well.
  • Incubate 2.5 h at RT.
  • Add 100 µl prepared streptavidin solution.
  • Incubate 45 min at RT.
  • Add 100 µl TMB One-Step Substrate Reagent to each well.
  • Incubate 30 min at RT.
  • Add 50 µl Stop Solution to each well.
  • Read plate at 450 nm immediately.
  • Storage/Stability

    Standard, Biotinylated Angiotensin II peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

    Antigen Information

    Gene Symbol:
    • AGT

    Product Specifications

    Size:
    1, 2, or 5 x 96-Well Strip Microplate Kit

    Array/ELISA Format

    Species Detected:
    • Human
    • Mouse
    • Rat
    Solid Support:
    96-well Microplate
    Design Principle:
    • Competition-based
    Quantitative/Semi-Quantitative:
    Quantitative
    Method of Detection:
    Colorimetric
    Compatible Sample Types:
    • Serum

    1. Pandey VG., Jain S., Rana A., et al. Dexamethasone promotes hypertension by allele-specific regulation of the human angiotensinogen gene. J Biol Chem. 2015 Feb 27;290(9):5749-58. doi: 10.1074/jbc.M114.601922.
      Species: Mouse
      Sample Type: Plasma

    2. Najafipour H., Vakili A., Shahouzehi B., et al. Investigation of changes in apelin receptor mRNA and protein expression in the myocardium and aorta of rats with two-kidney, one-clip (2K1C) Goldblatt hypertension. J Physiol Biochem. 2015 Jun;71(2):165-75. doi: 10.1007/s13105-015-0394-z
      Species: Rat
      Sample Type: Serum

    3. Zheng Z., Cheng C., Dong R., et al. Advanced glycation end products upregulate angiopoietin-like protein 4 expression by activating the renin-angiotensin system in endothelial cells. Biomed Rep. 2015 Jul;3(4):578-582
      Species: Human
      Sample Type: Conditioned Media

    4. Youn J., Zhou J, Cai H. Bone Morphogenic Protein 4 Mediates NOX1-Dependent eNOS Uncoupling, Endothelial Dysfunction, and COX2 Induction in Type 2 Diabetes Mellitus. Mol Endocrinol. 2015 Aug;29(8):1123-33. doi: 10.1210/ME.2014-1313.
      Species: Mouse
      Sample Type: Plasma

    5. Xue Q., Chen P., Xiongxiong L., et al. Maternal High-Fat Diet Causes a Sex-Dependent Increase in AGTR2 Expression and Cardiac Dysfunction in Adult Male Rat Offspring. Biol Reprod. 2015 Aug;93(2):49. doi: 10.1095/biolreprod.115.129916
      Species: Rat
      Sample Type: Plasma

    6. Sugimura-Wakayama Y., Katafiri W., Osugi M., et al. Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth. Stem Cells Dev. 2015 Nov 15;24(22):2687-99. doi: 10.1089/scd.2015.0104.
      Species: Rat
      Sample Type: Plasma

    7. Joshi S., Gupta N., Khan R., et al. Interrelationship between angiogenesis, inflammation and oxidative stress in Indian patients with multiple myeloma. Clin Transl Oncol. 2016 Feb;18(2):132-7. doi: 10.1007/s12094-015-1344-5.
      Species: Human
      Sample Type: Serum

    8. Hou J., Yan P., Guo T., et al. Cardiac stem cells transplantation enhances the expression of connexin 43 via the ANG II/AT1R/TGF-beta1 signaling pathway in a rat model of myocardial infarction. Exp Mol Pathol. 2015 Dec;99(3):693-701. doi: 10.1016/j.yexmp.2015.11.013
      Species: Rat
      Sample Type: Plasma

    9. Hou J., Yan P., Guo T., et al. Cardiac stem cells transplantation enhances the expression of connexin 43 via the ANG II/AT1R/TGF-beta1 signaling pathway in a rat model of myocardial infarction. Exp Mol Pathol. 2015 Dec;99(3):693-701. doi: 10.1016/j.yexmp.2015.11.013
      Species: Rat
      Sample Type: Tissue Lysate

    10. Dhar, Priyanka, Shashi B. Singh, and Vijay K. Sharma. "Autonomic Cardiovascular Responses in Acclimatized Lowlanders on Prolonged Stay at High Altitude: A Longitudinal Follow Up Study." PLoS One. PLoS One, 03 Jan. 2014. Web.
      Species: Human
      Sample Type: Serum

    11. Emdin M., et al. Biomarkers of activation of renin-angiotensin-aldosterone system heart failure: how useful, how feasible? Clinica Chimica Acta Dec 2014, epub ahead of print. DOI: 10.1016/j.cca.2014.10.031
      Species: Human
      Sample Type: N/A

    12. Hao L., Wang X., Cheng J., You S., Ma S., Zhong X., Quan L., Luo B. The upregulation of endothelin-1 and downregulation of miRNA-125a-5p, -155 and -199a/b-3p in human atherosclerotic coronary artery. Cardiovascular Pathology - 07 April 2014 [Epub Ahead of Print]
      Species: Human
      Sample Type: Pericardial Fluid

    13. Azibani G
      Species: Mouse
      Sample Type: Plasma

    14. Azibani G
      Species: Mouse
      Sample Type: Tissue Lysate

    15. Mascareno E, Galatioto J, Rozenberg I, et al. Cardiac Lineage Protein-1 (CLP-1) Regulates Cardiac Remodeling via Transcriptional Modulation of Diverse Hypertrophic and Fibrotic Responses and Angiotensin II-transforming Growth Factor β (TGF-β1) Signaling Axis. The Journal of Biological Chemistry 2012;287(16):13084-13093. doi:10.1074/jbc.M111.288944.
      Species: Mouse
      Sample Type: Tissue Lysate

    16. Cheng C., Tang Y., Zheng Z., et al. Advanced glycation end-products activate the renin-angiotensin system through the RAGE/PI3-K signaling pathway in podocytes. Clin Invest Med. 2012 Oct 6;35(5):E282.
      Species: Mouse
      Sample Type: Conditioned Media

    17. Cheng C., Tang Y., Zheng Z., et al. Advanced glycation end-products activate the renin-angiotensin system through the RAGE/PI3-K signaling pathway in podocytes. Clin Invest Med. 2012 Oct 6;35(5):E282.
      Species: Mouse
      Sample Type: Cell Lysate

    18. Fouad AA., Al-Mulhim AS., Jresat I., Gomaa W. Therapeutic role of telmisartan against acetaminophen hepatotoxicity in mice. Eur J Pharmacol. 2012 Oct 15;693(1-3):64-71. doi: 10.1016/j.ejphar.2012.07.049.
      Species: Mouse
      Sample Type: Tissue Lysate

    19. Fouad AA., Al-Mulhim AS., Jresat I. Telmisartan treatment attenuates arsenic-induced hepatotoxicity in mice. Toxicology. 2012 Oct 28;300(3):149-57. doi: 10.1016/j.tox.2012.06.015.
      Species: Mouse
      Sample Type: Tissue Lysate

    20. Wang Y., Deb D., Zhang Z., et al. Vitamin D receptor signaling in podocytes protects against diabetic nephropathy. J Am Soc Nephrol. 2012 Dec;23(12):1977-86. doi: 10.1681/ASN.2012040383.
      Species: Mouse
      Sample Type: Conditioned Media

    21. Najafipour H., Soltani Hekmat A., Nekooian A., Esmaeili-Mahini S. Apelin receptor expression in ischemic and non- ischemic kidneys and cardiovascular responses to apelin in chronic two-kidney-one-clip hypertension in rats. Regul Pept. 2012 Oct 10;178(1-3):43-50. doi: 10.1016/j.regpep.2012.06.006.
      Species: Rat
      Sample Type: Plasma

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    Usage

    This product is furnished for LABORATORY RESEARCH USE ONLY.
    Not for diagnostic or therapeutic use.

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