Save 20%

Human ANGII EIA

RayBio® Human/Mouse/Rat Angiotensin II EIA Kit optimized for serum. Competition-based ELISA on a 96-well strip plate.

Old price: $380.00
$304.00
You save: $76.00 (20%)
EIA-ANGII-1
+

Antigen Information

Accession Number:
  • P01019
Gene ID:
  • 183
Gene Symbols:
  • AGT
  • SERPINA8
Protein Name & Synonyms:
Angiotensinogen (Serpin A8) [Cleaved into: Angiotensin-1 (Angiotensin 1-10) (Angiotensin I) (Ang I) Angiotensin-2 (Angiotensin 1-8) (Angiotensin II) (Ang II) Angiotensin-3 (Angiotensin 2-8) (Angiotensin III) (Ang III) (Des-Asp[1]-angiotensin II) Angiotensin-4 (Angiotensin 3-8) (Angiotensin IV) (Ang IV) Angiotensin 1-9 Angiotensin 1-7 Angiotensin 1-5 Angiotensin 1-4]

Assay Format

Detection Range:
0.1-1,000 pg/ml
Recommended Dilution (serum & plasma):
Human: 2X / Mouse: 2X / Rat: 2X
Sensitivity:
0.3 pg/ml
Specificity:
This kit can theoretically detect all active angiotensins, including ANGI, ANGII, ANGIII and ANGIV as well as inactive angiotensinogen.

Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, NPY and APC.
Number of Targets Detected:
1
Species Detected:
  • Human
  • Mouse
  • Rat
Compatible Sample Types:
  • Serum
Design Principle:
  • Competition-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Introduction

Angiotensin, a key player in the renin-angiotensin system, is a peptide hormone that causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenal cortex. It is derived from the precursor molecule angiotensinogen produced in the liver.

Angiotensin II is formed from Angiotensin I, which is removed of two terminal residues by the enzyme Angiotensin-converting enzyme (ACE). Angiotensin II acts as an endocrine, autocrine/ paracrine, and intracrine hormone. Angiotensin II is degraded to angiotensin III by angiotensinases that are located in red blood cells and the vascular beds of most tissues. It has a half-life in circulation of around 30 seconds, while in tissue, it may be as long as 15-30 minutes.

The effect of obesity on Angiotensin II has recently been reported. Obese patients show heightened renal vasodilation to blockade of the renin-angiotensin system, suggesting deficits in vascular responses to angiotensin II. This may due to increased reactivity of renal vasoconstriction to ANG II.

Angiotensin II has been associated with a number of important physiological processes in heart, brain, adrenal gland and kidney. For cardiovascular effect, Angiotensin II is a potent direct vasoconstrictor, constricting arteries and veins and increasing blood pressure. It is also the most important Gq stimulator of the heart during hypertrophy. For neural effects, Angiotensin II increases thirst sensation (dipsogen) through the subfornical organ (SFO) of the brain, decreases the response of the baroreceptor reflex, and increases the desire for salt. It increases secretion of ADH in the posterior pituitary and secretion of ACTH in the anterior pituitary. For adrenal effects, Angiotensin II acts on the adrenal cortex, causing it to release aldosterone. For renal effects, Angiotensin II has a direct effect on the proximal tubules to increase Na+ absorption.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
  • Prepare all reagents, samples and standards as instructed.
  • Add 100 µl detection antibody to each well.
  • Incubate 1.5 h at RT or O/N at 4°C.
  • Add 100 µl standard or sample to each well.
  • Incubate 2.5 h at RT.
  • Add 100 µl prepared streptavidin solution.
  • Incubate 45 min at RT.
  • Add 100 µl TMB One-Step Substrate Reagent to each well.
  • Incubate 30 min at RT.
  • Add 50 µl Stop Solution to each well.
  • Read plate at 450 nm immediately.

Storage/Stability

Standard, Biotinylated Angiotensin II peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
  1. Pandey VG., Jain S., Rana A., et al. Dexamethasone promotes hypertension by allele-specific regulation of the human angiotensinogen gene. J Biol Chem. 2015 Feb 27;290(9):5749-58. doi: 10.1074/jbc.M114.601922.
    Species: Mouse
    Sample type: Plasma
  2. Najafipour H., Vakili A., Shahouzehi B., et al. Investigation of changes in apelin receptor mRNA and protein expression in the myocardium and aorta of rats with two-kidney, one-clip (2K1C) Goldblatt hypertension. J Physiol Biochem. 2015 Jun;71(2):165-75. doi: 10.1007/s13105-015-0394-z
    Species: Rat
    Sample type: Serum
  3. Zheng Z., Cheng C., Dong R., et al. Advanced glycation end products upregulate angiopoietin-like protein 4 expression by activating the renin-angiotensin system in endothelial cells. Biomed Rep. 2015 Jul;3(4):578-582
    Species: Human
    Sample type: Conditioned Media
  4. Youn J., Zhou J, Cai H. Bone Morphogenic Protein 4 Mediates NOX1-Dependent eNOS Uncoupling, Endothelial Dysfunction, and COX2 Induction in Type 2 Diabetes Mellitus. Mol Endocrinol. 2015 Aug;29(8):1123-33. doi: 10.1210/ME.2014-1313.
    Species: Mouse
    Sample type: Plasma
  5. Xue Q., Chen P., Xiongxiong L., et al. Maternal High-Fat Diet Causes a Sex-Dependent Increase in AGTR2 Expression and Cardiac Dysfunction in Adult Male Rat Offspring. Biol Reprod. 2015 Aug;93(2):49. doi: 10.1095/biolreprod.115.129916
    Species: Rat
    Sample type: Plasma
  6. Sugimura-Wakayama Y., Katafiri W., Osugi M., et al. Peripheral Nerve Regeneration by Secretomes of Stem Cells from Human Exfoliated Deciduous Teeth. Stem Cells Dev. 2015 Nov 15;24(22):2687-99. doi: 10.1089/scd.2015.0104.
    Species: Rat
    Sample type: Plasma
  7. Joshi S., Gupta N., Khan R., et al. Interrelationship between angiogenesis, inflammation and oxidative stress in Indian patients with multiple myeloma. Clin Transl Oncol. 2016 Feb;18(2):132-7. doi: 10.1007/s12094-015-1344-5.
    Species: Human
    Sample type: Serum
  8. Hou J., Yan P., Guo T., et al. Cardiac stem cells transplantation enhances the expression of connexin 43 via the ANG II/AT1R/TGF-beta1 signaling pathway in a rat model of myocardial infarction. Exp Mol Pathol. 2015 Dec;99(3):693-701. doi: 10.1016/j.yexmp.2015.11.013
    Species: Rat
    Sample type: Tissue Lysate
  9. Dhar, Priyanka, Shashi B. Singh, and Vijay K. Sharma. "Autonomic Cardiovascular Responses in Acclimatized Lowlanders on Prolonged Stay at High Altitude: A Longitudinal Follow Up Study." PLoS One. PLoS One, 03 Jan. 2014. Web.
    Species: Human
    Sample type: Serum
  10. Emdin M., et al. Biomarkers of activation of renin-angiotensin-aldosterone system heart failure: how useful, how feasible? Clinica Chimica Acta Dec 2014, epub ahead of print. DOI: 10.1016/j.cca.2014.10.031
    Species: Human
    Sample type: N/A
Kit works great!

SUNY Buffalo
Write A Review

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Amazon Gift Card Program

Customers Also Bought Customers Also Bought