Human ANGPT1 ELISA

RayBio® Human Angiopoietin-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$723.60  
ELH-Angiopoietin1-2
+

Assay Format

Detection Range:
30 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma):
3 - 30 fold
Sensitivity:
30 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, Angiopoietin 2, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q15389
Gene ID:
  • 284
Gene Symbols:
  • ANGPT1
  • KIAA0003
Protein Name & Synonyms:
Angiopoietin-1 (ANG-1)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Tasaki, Yuko, et al. "Disruption of vascular endothelial homeostasis in systemic juvenile idiopathic arthritis-associated macrophage activation syndrome: The dynamic roles of angiopoietin-1 and-2." Cytokine 80 (2016): 1-6.
    Species: Human
    Sample type: Plasma
  2. Khan R, Sharma M, Kumar L, et al. Interrelationship and expression profiling of cyclooxygenase and angiogenic factors in Indian patients with multiple myeloma. Ann Hematol. 2013 Jan;92(1):101-109.
    Species: Human
    Sample type: Serum (Min. detectable dose MDD of VEGF, HGF and Ang-1 was less than 20, 3, and 30 pg/ml.)
  3. Synergistic effect of vascular endothelial growth factor and angiopoietin-2 on progression free survival in multiple myeloma
    Species: Human
    Sample type: Serum (sensitivity <30pg/ml)
  4. Koksal BT., Ozbek OY., Bayraktar N., et al. Evaluation of angiopoietin 1 and 2, vascular endothelial growth factor, and tumor necrosis factor alpha levels in asthmatic children. Allergy and Asthma Proceedings, Volume 35, Number 6, November/December 2014, pp. 482-488(7). http://dx.doi.org/10.2500/aap.2014.35.3793
    Species: Human
    Sample type: Serum (Asthmatic Children)
  5. Karap?nar, Hekim, et al. "Serum levels of angiopoietin-1 in patients with pulmonary hypertension due to mitral stenosis." Heart and vessels 26.5 (2011): 536-541.
    Species: Human
    Sample type: Serum
  6. Chen S., Li H., Zhang C., et al. Urinary Angiopoietin-2 Is Associated with Albuminuria in Patients with Type 2 Diabetes Mellitus. International Journal of Endocrinology Volume 2014, Article ID 163120, 7 pages .http://dx.doi.org/10.1155/2014/163120
    Species: Human
    Sample type: Serum (Type 2 Diabetes Mellitus)
  7. Chen S., Li H., Zhang C., et al. Urinary Angiopoietin-2 Is Associated with Albuminuria in Patients with Type 2 Diabetes Mellitus. International Journal of Endocrinology Volume 2014, Article ID 163120, 7 pages .http://dx.doi.org/10.1155/2014/163120
    Species: Human
    Sample type: Urine (Type 2 Diabetes Mellitus)
  8. Liu, Xudong, Bingfang Zeng, and Changqing Zhang. "Osteogenic and angiogenic effects of mesenchymal stromal cells with co-transfected human Ang-1 gene and BMP2 gene." Biotechnology letters 33.10 (2011): 1933-1938.
    Species: Human
    Sample type: Conditioned Media (MSCs)
  9. Gok, Mahmut, et al. "Relationship of ultrasonographic findings with synovial angiogenesis modulators in different forms of knee arthritides." Rheumatology international 33.4 (2013): 879-885.
    Species: Human
    Sample type: Synovial Fluid (knee arthritis patients)
  10. Fernandez-Yunquera A., et al. Everolimus immunosuppression reduces the serum expression of fibrosis markers in liver transplant recipients. World J Transplant 2014; 4(2): 133-140. http://dx.doi.org/10.5500/wjt.v4.i2.133
    Species: Human
    Sample type: Serum (Liver Transplant patients)

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

Recently Viewed Recently Viewed

Customers Also Bought Customers Also Bought