RayBio® Human Angiopoietin-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • O15123
Gene ID:
  • 285
Gene Symbols:
  • ANGPT2
Protein Name & Synonyms:
Angiopoietin-2 (ANG-2)
Target Species:
  • Human

Assay Format

Detection Range:
10 pg/ml - 3000 pg/ml
Recommended Dilution (serum & plasma):
3 - 20 fold
10 pg/ml

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This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, Angiopoietin-1, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GCSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Tasaki, Yuko, et al. "Disruption of vascular endothelial homeostasis in systemic juvenile idiopathic arthritis-associated macrophage activation syndrome: The dynamic roles of angiopoietin-1 and-2."?Cytokine?80 (2016): 1-6.
    Species: Human
    Sample type: Plasma
  2. Palud A., Parmentier-Decrucq E., Pastre J., et al. Evaluation of endothelial biomarkers as predictors of organ failures in septic shock patients. Cytokine. 2015 Jun;73(2):213-8. doi: 10.1016/j.cyto.2015.02.013.
    Species: Human
    Sample type: Plasma
  3. Andrukhov O., Germperli A., Tang Y., et al. Effect of different enamel matrix derivative proteins on behavior and differentiation of endothelial cells. Dent Mater. 2015 Jul;31(7):822-32. doi: 10.1016/
    Species: Human
    Sample type: Conditioned Media
  4. He F., Li H., Huang Q., et al. Tumor Necrosis Factor-Alpha and 8-Hydroxy-2'-Deoxyguanosine are Associated with Elevated Urinary Angiopoietin-2 Level in Type 2 Diabetic Patients with Albuminuria. Kidney Blood Press Res. 2015;40(4):355-65. doi: 10.1159/000368510
    Species: Human
    Sample type: Urine
  5. Pul R., Yildiz O., Morbiducci F., et al. CSF Levels of Angiopoietin-2 Do Not Differ between Patients with CSF Fluid Leakage Syndrome and Controls. Dis Markers. 2015;2015:343818. doi: 10.1155/2015/343818.
    Species: Human
    Sample type: CSF
  6. Chen S., Li H., Zhang C., et al. Urinary Angiopoietin-2 Is Associated with Albuminuria in Patients with Type 2 Diabetes Mellitus. International Journal of Endocrinology Volume 2014, Article ID 163120, 7 pages .
    Species: Human
    Sample type: Urine
  7. Koksal BT., Ozbek OY., Bayraktar N., et al. Evaluation of angiopoietin 1 and 2, vascular endothelial growth factor, and tumor necrosis factor alpha levels in asthmatic children. Allergy and Asthma Proceedings, Volume 35, Number 6, November/December 2014, pp. 482-488(7).
    Species: Human
    Sample type: Serum
  8. Koob T., Lim J., Massee M., Zabek N., Rennet R., Gurtner G., Li W. Angiogenic properties of dehydrated human amnion/chorion allografts. Vascular Cell 2014,6:10 doi:10.1186/2045-824X-6-10 [Epub Ahead of Print]therapeutic potential for soft tissue repair and regeneration
    Species: Human
    Sample type: Tissue Lysate
  9. Mohsen M., et al. Angiogenic output in viral hepatitis, C and B, and HCV-associated hepatocellular carcinoma . Alexandria Journal of Medicine Available online 4 July 2014 In Press, Corrected Proof. DOI: 10.1016/j.ajme.2014.06.003
    Species: Human
    Sample type: Serum
  10. Ayten O, Tas D, Demirer E, et al. Angiopoietin 2 levels in serum and bronchial lavage fluids and their relationship with cancer stages in lung cancer patients. Thoracic Cancer. 2013;4(1):20-26.
    Species: Human
    Sample type: Serum

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