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Human ANGPTL3 ELISA

RayBio® Human ANGPTL3 ELISA Kit for serum, plasma, and cell culture supernatant samples.

$421.00
Manual SDS
ELH-ANGPTL3-1
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Antigen Information

Accession Number:
  • Q9Y5C1
Gene ID:
  • 27329
Gene Symbols:
  • ANGPT5
  • ANGPTL3
  • UNQ153/PRO179
Protein Name & Synonyms:
Angiopoietin-related protein 3 (Angiopoietin-5) (ANG-5) (Angiopoietin-like protein 3)
Target Species:
  • Human

Assay Format

Detection Range:
8.2 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
200 fold
Sensitivity:
8.2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: human beta IG-H3, CA9, Cathepsin B, CD23, CD26 (DPPIV), CHI3L1, Coagulation Factor III, CTLA-4, Dkk-4, EDA-A2, Erythropoietin R, FGF-6, FGF-9, FOLH1 (PSMA), Gas 1, IGFBP-5, IL-38 (IL-1 F10), IL-36 Ra (IL-1 F5), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-1 R5 (IL-18 R alpha), IL-10 R alpha, IL-17C, IL-18, IL-20, IL-34, IL-5 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, SIGIRR, TGF beta R3,TWEAK.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Manual SDS
  1. Bassyouni I., Sharaf M., Wali I., Mansour H. Clinical significance of Angiopoietin-1 in Behcet's disease patients with vascular involvement. Heart Vessels. 2015 May 12. [Epub ahead of print]
    Species: Human
    Sample type: Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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