RayBio® Human ANGPTL4 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
20 pg/ml - 20000 pg/ml
Recommended Dilution (serum & plasma):
3 fold
20 pg/ml

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This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q9BY76
Gene ID:
  • 51129
Gene Symbols:
  • ARP4
  • PGAR
  • PP1158
  • PSEC0166
  • UNQ171/PRO197
Protein Name & Synonyms:
Angiopoietin-related protein 4 (Angiopoietin-like protein 4) (Hepatic fibrinogen/angiopoietin-related protein) (HFARP)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Drager LF, Yao Q, Hernandez KL, et al. Chronic Intermittent Hypoxia Induces Atherosclerosis via Activation of Adipose Angiopoietin-like 4. Am. J. Respir. Crit. Care Med. 2013 Jan 17;
    Species: Human
    Sample type: Serum (cited in supplemental data & methods)
  2. Angelini PD, Zacarias Fluck MF, Pederson K, et al. Constitutive HER2 signaling promotes breast cancer metastasis through cellular senescence. Cancer Res. 2013;73(1); 450-458.
    Species: Human
    Sample type: Conditioned Media (MCF7 (breast cancer) cell line, treated vs. untreated)
  3. Angelini PD, Zacarias Fluck MF, Pederson K, et al. Constitutive HER2 signaling promotes breast cancer metastasis through cellular senescence. Cancer Res. 2013;73(1); 450-458.
    Species: Human
    Sample type: Serum
  4. Wang, Ying, et al. "Severity of diabetes governs vascular lipoprotein lipase by affecting enzyme dimerization and disassembly." Diabetes 60.8 (2011): 2041-2050.
    Species: Rat
    Sample type: Serum
  5. Adhikary, Till, et al. "Inverse PPAR?/? agonists suppress oncogenic signaling to the ANGPTL4 gene and inhibit cancer cell invasion." Oncogene 32.44 (2013): 5241.
    Species: Human
    Sample type: Conditioned Media (MDA-MB-231 cells)
  6. Clasen BFF, Krusenstjerna-Hafstrom T, Vendelbo MH, et al. Gene Expression in Skeletal Muscle after an Acute Intravenous GH Bolus in Human Subjects: Identification of a Mechanism Regulating ANGPTL4. J Lipid Res. 2013;57(7):1988-1997.
    Species: Human
    Sample type: Serum (healthy subjects vs. subjects with GH deficiency)
  7. Basak S, Duttaroy AK. cis-9,trans-11 conjugated linoleic acid stimulates expression of angiopoietin like-4 in the placental extravillous trophoblast cells. Biochim Biophys Acta. 2013;1831(4):834-843.
    Species: Human
    Sample type: Conditioned Media (HTR8 cells)
  8. Basak, Sanjay, and Asim K. Duttaroy. "Effects of fatty acids on angiogenic activity in the placental extravillious trophoblast cells." Prostaglandins, Leukotrienes and Essential Fatty Acids 88.2 (2013): 155-162.
    Species: Human
    Sample type: Conditioned Media (HTR8 cells - fatty acid effects)
  9. Baranowski, T., et al. "Serum levels of the adipokine fasting-induced adipose factor/angiopoietin-like protein 4 depend on renal function." Hormone and metabolic research 43.02 (2011): 117-120.
    Species: Human
    Sample type: Serum
  10. Wadood S., Saihood Y., Mohammed Z. Determination of Angiopoietin Like Protein-4 Levels and Some Parameters in Iraqi Patients with Type 2 Diabetes Mellitus. Journal of Al-Nahrain University, Vol.18 (2), June, 2015, pp.33-43
    Species: Human
    Sample type: Plasma (Type 2 Diabetes Iraqi patients)
Standards worked fine. Urine samples without dilution are good for this marker

University of Houston
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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