RayBio® Human/Mouse/Rat Atrial Natriuretic Peptide EIA Kit optimized for serum and cell culture medium. Competition-based ELISA on a 96-well strip plate.


Assay Format

Detection Range:
0.1-1,000 pg/ml
Recommended Dilution (serum & plasma):
Human: 4X / Mouse: 4X / Rat: 4X
1.02 pg/ml
This kit targets the common sequence of human, mouse and rat, and thus may be used to detect ANP expression in all these species with high specificity and sensitivity.

Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Number of Targets Detected:
Species Detected:
  • Human
  • Mouse
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Serum
Design Principle:
  • Competition-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P01160
Gene ID:
  • 4878
Gene Symbols:
  • ANP
  • NPPA
  • PND
Protein Name & Synonyms:
Natriuretic peptides A (CDD-ANF) (Prepronatriodilatin) [Cleaved into: Cardiodilatin-related peptide (CDP) Atrial natriuretic factor (ANF) (Atrial natriuretic peptide) (ANP)]


Atrial natriuretic peptide (ANP) is a 28-amino acid peptide hormone secreted by cardiac myocytes of the atrium. ANP plays an important role in the homeostatic regulation of body water, sodium, potassium and fat, by acting to reduce the water, sodium and adipose loads on the circulatory system, thus reducing blood pressure.

ANP peptide contains a 17-amino acid ring which is formed by a disulfide bond between two cysteine residues at positions 7 and 23. ANP is closely related to BNP (brain natriuretic peptide) and CNP (C-type natriuretic peptide), which all share the same amino acid ring.

The mechanism of ANP-induced vasodilatation is through binding to a specific set of ANP receptors. Receptor-agonist binding causes a reduction in blood volume and therefore a reduction in cardiac output and systemic blood pressure. Lipolysis is increased and renal sodium reabsorption is decreased. The overall effect of ANP on the body is to counter increases in blood pressure and volume caused by the renin-angiotensin system.

In addition to its vasodilatation effect, ANP also serves as a adipokine. Studies have shown that ANP Increases the release of free fatty acids from adipose tissue, activates adipocyte plasma membrane NPR-A receptors, and increases intracellular cGMP levels that induce the phosphorylation of a hormone-sensitive lipase and perilipin A.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
  • Prepare all reagents, samples and standards as instructed.
  • Add 100 µl detection antibody to each well.
  • Incubate 1.5 h at RT or O/N at 4°C.
  • Add 100 µl standard or sample to each well.
  • Incubate 2.5 h at RT.
  • Add 100 µl prepared streptavidin solution.
  • Incubate 45 min at RT.
  • Add 100 µl TMB One-Step Substrate Reagent to each well.
  • Incubate 30 min at RT.
  • Add 50 µl Stop Solution to each well.
  • Read plate at 450 nm immediately.
  • Storage/Stability

    Standard, Biotinylated Atrial natriuretic peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    1. Staedtke V., Bai R., Kim K., et al. Disruption of a self-amplifying catecholamine loop reduces cytokine release syndrome. Nature. 2018 Dec;564(7735):273-277. doi: 10.1038/s41586-018-0774-y
      Species: Mouse
      Sample type: Plasma
    2. Staedtke V, Bai RY, Kim K, Darvas M, Davila ML, Riggins GJ, Rothman PB, Papadopoulos N, Kinzler KW, Vogelstein B, Zhou S. Disruption of a self-amplifying catecholamine loop reduces cytokine release syndrome. Nature. 2018 Dec;564(7735):273-277. doi: 10.1038/s41586-018-0774-y. Epub 2018 Dec 12. PMID: 30542164; PMCID: PMC6512810.
      Species: Human
      Sample type: Conditioned Media
    3. Asmar A., Simonsen L., Asmar M., et al. Renal extraction and acute effects of glucagon-like peptide-1 on central and renal hemodynamics in healthy men. Am J Physiol Endocrinol Metab. 2015 Apr 15;308(8):E641-9. doi: 10.1152/ajpendo.00429.2014.
      Species: Human
      Sample type: Plasma
    4. Egom E., Vella K., Hua R., et al. Impaired sinoatrial node function and increased susceptibility to atrial fibrillation in mice lacking natriuretic peptide receptor C. J Physiol 593.5 (2015) pp 1127-1146
      Species: Mouse
      Sample type: Plasma
    5. Hamdani N., et al. Left ventricular diastolic dysfunction and myocardial stiffness in diabeticmice is attenuated by inhibition of dipeptidyl peptiase 4. Cardiovascular Research (2014) 104, 423-431. doi:10.1093/cvr/cvu223
      Species: Mouse
      Sample type: Serum
    6. Kim M, Platt MJ, Shibasaki T, Quaggin SE, Backx PH, et al. GLP-1 receptor activation and Epac2 link atrial natriuretic peptide secretion to control of blood pressure. Nat Med. 2013;19:567-575.
      Species: Mouse
      Sample type: Serum
    7. Samillan V., et al. Combination of erythropoietin and sildenafil can effectively attenuate hypoxia-induced pulmonary hypertension in mice. Pulm Circ. 2013 Dec;3(4):898-907. doi: 10.1086/674758
      Species: Mouse
      Sample type: Serum
    8. Muchir A., et al. Treatment with selumetinib preserves cardiac function and improves survival in cardiomyopathy caused by mutation in the lamin A/C gene. Cardiovasc Res. 2012 Feb 1;93(2):311-9. doi: 10.1093/cvr/cvr301
      Species: Mouse
      Sample type: Serum
    9. Ku HC., et al. DPP4 deficiency preserves cardiac function via GLP-1 signaling in rats subjected to myocardial ischemia/reperfusion. Naunyn Schmiedebergs Arch Pharmacol. 2011 Aug;384(2):197-207. doi: 10.1007/s00210-011-0665-3.
      Species: Rat
      Sample type: Plasma

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