Custom Human ApoA SpeedELISA

$633.00
ELHS-ApoA

RayBio® Custom Human ApoA SpeedELISA Kit. 6 - 8 week lead time.

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Antigen Information

Accession Number:
  • P02647
Gene ID:
  • 335
Gene Symbol:
  • APOA1
Protein Name & Synonyms:
Apolipoprotein A-I (Apo-AI) (ApoA-I) (Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I (ProapoA-I) Truncated apolipoprotein A-I (Apolipoprotein A-I(1-242))]

Assay Format

Number of Targets Detected:
1
Species Detected:
  • Human
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

  • Compressed workflow with only one wash step and three incubation steps
  • 3 hours total processing time
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Application Notes

Kit Components
  • Streptavidin-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Capture Antibody
  • HRP Conjugated Detection Antibody
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 70 µl of the Antibody Cocktail to each well.
  3. Incubate 10 minutes at RT.
  4. Add 50 µl of sample or standard to each well.
  5. Incubate 2 h at RT.
  6. Wash Wells
  7. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  8. Incubate 30 min at RT.
  9. Add 50 µl of Stop Solution to each well.
  10. Read at 450 nm immediately.

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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