Human Apoptosis Array C1

RayBio® C-Series Human Apoptosis Antibody Array 1 Kit. Detects 43 Human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

$512.00  
AAH-APO-1-4
+

Assay Format

Pathway:
  • Akt Signaling
  • IGF Signaling
  • p53 Signaling
Number of Targets Detected:
43
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
BAD | BAX | BCL2 | BCL2L11 | BCL2L2 | BID | BIRC3 | BIRC5 | BIRC7 | CASP3   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bad
bax
  • Gene Symbol: BAX
  • Gene ID: 581
  • Accession #: Q07812
bcl-2
  • Gene Symbol: BCL2
  • Gene ID: 596
  • Accession #: P10415
bcl-w
  • Gene Symbol: BCL2L2
  • Gene ID: 599
  • Accession #: Q92843
BID
BIM
  • Gene Symbol: BCL2L11
  • Gene ID: 10018
  • Accession #: Q43521
Caspase-3
  • Gene Symbol: CASP3
  • Gene ID: 836
  • Accession #: P42574
Caspase-8
  • Gene Symbol: CASP8
  • Gene ID: 841
  • Accession #: Q14790
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
cIAP-2
Cytochrome C
DR6
(TNFRSF21)
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
HSP27
HSP60
  • Gene Symbol: HSPA14
  • Gene ID: 51182
  • Accession #: Q0VDF9
HSP70
HTRA2
IGF-1
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-5
IGFBP-6
IGF-1 R
livin
p21
  • Gene Symbol: CDKN1A
  • Gene ID: 1026
  • Accession #: P38936
p27
p53
SMAC
Survivin
(BIRC5)
  • Gene Symbol: BIRC5
  • Gene ID: 332
  • Accession #: O15392
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R1
(TNFRSF10A/DR4)
TRAIL R2
(TNFRSF10B/DR5)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
XIAP

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Apoptosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Sun Y., Xiao Y., Sun H., et al. miR-27a regulates vascular remodeling by targeting endothelial cells’ apoptosis and interaction with vascular smooth muscle cells in aortic dissection, Theranostics. 2019; 9(25): 7961–7975.
    Species: Human
    Sample type: Cell Lysate (HUVEC and HASMC cell coculture)
  2. Wu B., Chu X., Feng C., et al. Heat shock protein gp96 decreases p53 stability by regulating Mdm2 E3 ligase activity in liver cancer. Cancer Lett. 2015 Apr 10;359(2):325-34. doi: 10.1016/j.canlet.2015.01.034.
    Species: Human
    Sample type: Cell Lysate (gp96 knock down cell lines)
  3. Spadavecchia S, Gonzalez-Lopez OD, Kyla Driscoll Carroll KD, Diana Palmeri D, Lukac DM. Convergence of KSHV Reactivation with EBV latency and cellular growth mediated by the Notch signaling pathway in co-infected cells. J Virol. 2010:84(20):10488-15000.
    Species: Human
    Sample type: Cell Lysate (transfected EREB2-5 cell extracts)
  4. Jackson R., Tilokee E., Latham N., et al. Paracrine Engineering of Human Cardiac Stem Cells With Insulin-Like Growth Factor 1 Enhances Myocardial Repair. J Am Heart Assoc. 2015 Sep 11;4(9):e002104. doi: 10.1161/JAHA.115.002104.
    Species: Human
    Sample type: Cell Lysate (IGF-1 lentiviral system in explant derived stem cells.)
  5. Jabir, M., Sahib, U., Taqi, Z., et al. Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-?B Translocation Inhibitor in SKOV-3 Cell Line. Int J Nanomedicine. 2020 Nov 17;15:9025-9047. doi: 10.2147/IJN.S276714. eCollection 2020.
    Species: Human
    Sample type: Cell Lysate (SKOV-3 ovarian cancer cell lines)
  6. Mohan S., Abdelwahab S., Kamalidehghan B., et al. Involvement of NF-?B and Bcl2/Bax signaling pathways in the apoptosis of MCF7 cells induced by a xanthone compound Pyranocycloartobiloxanthone A. Phytomedicine. 2012 Aug 15;19(11):1007-15. doi: 10.1016/j.phymed.2012.05.012.
    Species: Human
    Sample type: Cell Lysate (Pyranocycloartobiloxanthone A apoptosis model againts breast cancer cells (MCF7))
  7. Pastuszko P, Schears GJ, Pirzadeh A, et al. Effect of granulocyte-colony stimulating factor (G-CSF) on expression of selected proteins involved in regulation of apoptosis in the brain of newborn piglets following cardiopulmonary bypass (CPB) and deep hypothermic circulatory arrest (DHCA). The Journal of Thoracic and Cardiovascular Surgery. 2012;143(6):1436-1442. doi:10.1016/j.jtcvs.2012.01.018.
    Species: Porcine
    Sample type: Tissue Lysate (Porcine Brain lysate)
  8. Leslie K., Whitley G., Herse F., et al. Increased apoptosis, altered oxygen signaling, and antioxidant defenses in first-trimester pregnancies with high-resistance uterine artery blood flow. Am J Pathol. 2015 Oct;185(10):2731-41. doi: 10.1016/j.ajpath.2015.06.020.
    Species: Human
    Sample type: Tissue Lysate (Doppler Resistence index samples placental lysates)
  9. Zhang, D., Lee H., Cao Y., et al. miR-185 mediates lung epithelial cell death after oxidative stress. Am J Physiol Lung Cell Mol Physiol 310: L700–L710, 2016.
    Species: Human
    Sample type: Cell Lysate (Beas2B cells under hypoxic conditions.)
  10. Hu, Haiyan, et al. "The overexpression of hypomethylated miR-663 induces chemotherapy resistance in human breast cancer cells by targeting heparin sulfate proteoglycan 2 (HSPG2)." Journal of Biological Chemistry 288.16 (2013): 10973-10985.
    Species: Human
    Sample type: Cell Lysate (lysates of MDA-MB-231 cells with or without miR-663)

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Not for diagnostic or therapeutic use.

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