Human Apoptosis Array C1

RayBio® C-Series Human Apoptosis Antibody Array 1 Kit. Detects 43 Human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

$511.87  
AAH-APO-1-4
+

Antigen Information

Gene Symbols:
BAD | BAX | BCL2 | BCL2L11 | BCL2L2 | BID | BIRC3 | BIRC5 | BIRC7 | CASP3   View more

Assay Format

Pathway:
  • Akt Signaling
  • IGF Signaling
  • p53 Signaling
Number of Targets Detected:
43
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
bad
bax
  • Gene Symbol: BAX
  • Gene ID: 581
  • Accession #: Q07812
bcl-2
  • Gene Symbol: BCL2
  • Gene ID: 596
  • Accession #: P10415
bcl-w
  • Gene Symbol: BCL2L2
  • Gene ID: 599
  • Accession #: Q92843
BID
BIM
  • Gene Symbol: BCL2L11
  • Gene ID: 10018
  • Accession #: Q43521
Caspase-3
  • Gene Symbol: CASP3
  • Gene ID: 836
  • Accession #: P42574
Caspase-8
  • Gene Symbol: CASP8
  • Gene ID: 841
  • Accession #: Q14790
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
cIAP-2
Cytochrome C
DR6
(TNFRSF21)
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
HSP27
HSP60
  • Gene Symbol: HSPA14
  • Gene ID: 51182
  • Accession #: Q0VDF9
HSP70
HTRA2
IGF-1
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-5
IGFBP-6
IGF-1 R
livin
p21
  • Gene Symbol: CDKN1A
  • Gene ID: 1026
  • Accession #: P38936
p27
p53
SMAC
Survivin
(BIRC5)
  • Gene Symbol: BIRC5
  • Gene ID: 332
  • Accession #: O15392
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R1
(TNFRSF10A/DR4)
TRAIL R2
(TNFRSF10B/DR5)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
XIAP

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Apoptosis Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • Protease Inhibitor Cocktail
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Leslie K., Whitley G., Herse F., et al. Increased apoptosis, altered oxygen signaling, and antioxidant defenses in first-trimester pregnancies with high-resistance uterine artery blood flow. Am J Pathol. 2015 Oct;185(10):2731-41. doi: 10.1016/j.ajpath.2015.06.020.
    Species: Human
    Sample type: Tissue Lysate
  2. Ibrahim MY, Hashim NM, Mohan S, et al. Involvement of NF-?B and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, ?-mangostin, from Cratoxylum arborescens. Drug Design, Development and Therapy 2014;8:2193-2211. doi:10.2147/DDDT.S66574.
    Species: Human
    Sample type: Cell Lysate
  3. Karimian H., et al. Ferulago angulata activates intrinsic pathway of apoptosis in MCF-7 cells associated with G1 cell cycle arrest via involvement of p21/p27. Drug Design, Development and Therapy, Sept 2014, epub ahead of print.http://dx.doi.org/10.2147/DDDT.S68818
    Species: Human
    Sample type: Cell Lysate
  4. Lattuada D., et al. Proapoptotic Activity of a Monomeric Smac Mimetic on Human Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis. Inflammation, Sept 2014, epub ahead of print. DOI: 10.1007/s10753-014-0012-1
    Species: Human
    Sample type: Cell Lysate
  5. Paydar, M., Kamalidehghan, B. et al. evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models. Drug Design, Development and Therapy, Volume 2014:8 Pages 719 - 733. June 2014
    Species: Human
    Sample type: Cell Lysate
  6. Rahabi-Layachi H., et al. Distinct effects of inorganic phosphate on cell cycle and apoptosis in human vascular smooth muscle cells. J of Cell Phys. Accepted manuscript online: 27 JUN 2014. | DOI: 10.1002/jcp.24715
    Species: Human
    Sample type: Cell Lysate
  7. Wu J., et al. Blocking the bFGF/STAT3 interaction through specific signaling pathways induces apoptosis in glioblastoma cells. J Neurooncol publihsed online July 2014.DOI 10.1007/s11060-014-1529-8
    Species: Human
    Sample type: Cell Lysate
  8. Balao da Silva CM, Ortega Ferrusola C, Morillo Rodriguez A, et al. Sex sorting increases the permeability of the membrane of stallion spermatozoa. Anim Reprod Sci. 2013 May;138(3-4):241-251.
    Species: Equine
    Sample type: Cell Lysate
  9. Ng, Kuan-Beng, et al. "Induction of selective cytotoxicity and apoptosis in human T4-lymphoblastoid cell line (CEMss) by boesenbergin a isolated from boesenbergia rotunda rhizomes involves mitochondrial pathway, activation of caspase 3 and G2/M phase cell cycle arrest."?BMC complementary and alternative medicine?13.1 (2013): 41.
    Species: Human
    Sample type: Conditioned Media
  10. Hu, Haiyan, et al. "The overexpression of hypomethylated miR-663 induces chemotherapy resistance in human breast cancer cells by targeting heparin sulfate proteoglycan 2 (HSPG2)."?Journal of Biological Chemistry?288.16 (2013): 10973-10985.
    Species: Human
    Sample type: Cell Lysate

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Not for diagnostic or therapeutic use.

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