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| CODE | Number of Membranes | Price | ||
|---|---|---|---|---|
|
AAH-APOSIG-1-4
|
$579.66 | ||
|
|
AAH-APOSIG-1-8
|
$910.89 | ||
|
|
AAH-APOSIG-1-2
|
$414.04 |
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Antigen Information
Gene Symbols:
AKT1 | ATM | BAD | CASP3 | CASP7 | CHEK1 | CHEK2 | EIF2A | HSPB1 | MAP3K7| MAPK1 | MAPK3 | MAPK8 | NFKBIA | PARP1 | PSMD9 | RELA | SMAD2 | TP53 View more
Assay Format
Pathway:
- Akt Signaling
- HIF-1 alpha Signaling
- IGF Signaling
- JAK/STAT Signaling
- MAPK Signaling
- mTOR Signaling
- NFkB Signaling
- p53 Signaling
- PI3K-AKT Signaling
- TGF-beta Signaling
- Wnt/beta-Catenin Signaling
Number of Targets Detected:
19
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Membrane
Product Specifications
Size:
2, 4, or 8 Sample Kit
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Akt (P-Ser473)
(PKB (P-Ser473))
|
ATM (P-Ser1981)
|
BAD (P-Ser112)
|
Caspase-3 (Cleaved Asp175)
|
Caspase-7 (Cleaved Asp198)
|
|
CHK1 (P-Ser296)
|
CHK2 (P-Thr68)
|
eIF-2a (P-Ser52)
|
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
|
HSP27 (P-Ser82)
|
|
IKBa (P-Ser32)
|
JNK (P-Thr183/Tyr185)
|
NFKBP65 (P-Ser536)
|
PARP1 (Cleaved Asp214/Gly215)
|
p27 (P-Thr198)
|
|
P53 (P-Ser15)
|
SMAD2 (Ser245/250/255)
|
TAK1 (P-Ser412)
|
||
Typical Results
Figure 1: HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Staurosporine (STS) for 3 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Figure 2: Jurkat cells were either untreated (bottom panel) or treated (top panel) with Camptothecin(CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Apoptosis Signaling Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
-
Bao X, Shi J, Xie F, et al. Proteolytic Release of the p75NTR?Intracellular Domain by ADAM10 Promotes Metastasis and Resistance to Anoikis.?Cancer Res. 2018;78(9):2262-2276. doi:10.1158/0008-5472.CAN-17-2789
Species: Human
Sample type: Cell Lysates -
Smolensky D, Rhodes D, McVey DS, et al. High-Polyphenol Sorghum Bran Extract Inhibits Cancer Cell Growth Through ROS Induction, Cell Cycle Arrest, and Apoptosis.?J Med Food. 2018;21(10):990-998. doi:10.1089/jmf.2018.0008
Species: Human
Sample type: Cell Lysates
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
C-Series Testing Service
RayBiotech also provides a GLP-compliant CRO testing service for this kit. Fill out the form linked below and get your quote today!
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