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| CODE | Number of Membranes | Price | ||
|---|---|---|---|---|
|
AAH-APOSIG-1-4
|
$580.00 | ||
|
|
AAH-APOSIG-1-8
|
$911.00 | ||
|
|
AAH-APOSIG-1-2
|
$414.00 |
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Assay Format
Pathway:
- Akt Signaling
- HIF-1 alpha Signaling
- IGF Signaling
- JAK/STAT Signaling
- MAPK Signaling
- mTOR Signaling
- NFkB Signaling
- p53 Signaling
- PI3K-AKT Signaling
- TGF-beta Signaling
- Wnt/beta-Catenin Signaling
Number of Targets Detected:
19
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Membrane
Product Specifications
Size:
2, 4, or 8 Sample Kit
Protein Information
Gene Symbols:
AKT1 | ATM | BAD | CASP3 | CASP7 | CHEK1 | CHEK2 | EIF2A | HSPB1 | MAP3K7| MAPK1 | MAPK3 | MAPK8 | NFKBIA | PARP1 | PSMD9 | RELA | SMAD2 | TP53 View more
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Akt (P-Ser473) (PKB (P-Ser473))
| ATM (P-Ser1981)
| BAD (P-Ser112)
| Caspase-3 (Cleaved Asp175)
| Caspase-7 (Cleaved Asp198)
|
CHK1 (P-Ser296)
| CHK2 (P-Thr68)
| eIF-2a (P-Ser52)
| ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
| HSP27 (P-Ser82)
|
IKBa (P-Ser32)
| JNK (P-Thr183/Tyr185)
| NFKBP65 (P-Ser536)
| PARP1 (Cleaved Asp214/Gly215)
| p27 (P-Thr198)
|
P53 (P-Ser15)
| SMAD2 (Ser245/250/255)
| TAK1 (P-Ser412)
| ||
Typical Results
Figure 1: HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Staurosporine (STS) for 3 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Figure 2: Jurkat cells were either untreated (bottom panel) or treated (top panel) with Camptothecin(CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Apoptosis Signaling Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant¢ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
-
Bao X, Shi J, Xie F, et al. Proteolytic Release of the p75NTR Intracellular Domain by ADAM10 Promotes Metastasis and Resistance to Anoikis. Cancer Res. 2018;78(9):2262-2276. doi:10.1158/0008-5472.CAN-17-2789
Species: Human
Sample type: Cell Lysate (carcinoma cells) -
Vergani E, Dugo M, Cossa M, Frigerio S, Di Guardo L, Gallino G, Mattavelli I, Vergani B, Lalli L, Tamborini E, Valeri B, Gargiuli C, Shahaj E, Ferrarini M, Ferrero E, Gomez Lira M, Huber V, Vecchio MD, Sensi M, Leone BE, Santinami M, Rivoltini L, Rodolfo M, Vallacchi V. miR-146a-5p impairs melanoma resistance to kinase inhibitors by targeting COX2 and regulating NFkB-mediated inflammatory mediators. Cell Commun Signal. 2020 Sep 23;18(1):156. doi: 10.1186/s12964-020-00601-1. PMID: 32967672; PMCID: PMC7510138.
Species: Human
Sample type: Cell Lysate -
Hii, LW., Lim, SH.E., Leong, CO. et al. The synergism of Clinacanthus nutans Lindau extracts with gemcitabine: downregulation of anti-apoptotic markers in squamous pancreatic ductal adenocarcinoma. BMC Complement Altern Med 19, 257 (2019). https://doi.org/10.1186/s12906-019-2663-9
Species: Human
Sample type: Conditioned Media (cell culture media) -
Smolensky D, Rhodes D, McVey DS, et al. High-Polyphenol Sorghum Bran Extract Inhibits Cancer Cell Growth Through ROS Induction, Cell Cycle Arrest, and Apoptosis. J Med Food. 2018;21(10):990-998. doi:10.1089/jmf.2018.0008
Species: Human
Sample type: Cell Lysate (liver heptaocellular carcinoma)
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
C-Series Testing Service
RayBiotech also provides a GLP-compliant CRO testing service for this kit. Fill out the form linked below and get your quote today!
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