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Home/Search results/Products/Arrays/Antibody Arrays/C-Series/Apoptosis Arrays/Human Apoptosis Signaling Array C1

Manual / SDS

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Assay Format

Pathway:

Akt Signaling
HIF-1 alpha Signaling
IGF Signaling
JAK/STAT Signaling
MAPK Signaling
mTOR Signaling
NFkB Signaling
p53 Signaling
PI3K-AKT Signaling
TGF-beta Signaling
Wnt/beta-Catenin Signaling

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473) (PKB (P-Ser473)) Gene Symbol: AKT1 PKB RAC Gene ID: 207 Accession #: P31749 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA	ATM (P-Ser1981) Gene Symbol: ATM Gene ID: 472 Accession #: Q13315 ELISA Kit: RayBio Human Phospho-ATM (S1981) ELISA	BAD (P-Ser112) Gene Symbol: BAD BBC6 BCL2L8 Gene ID: 572 Accession #: Q92934 ELISA Kit: RayBio Human Phospho-BAD (S112) ELISA	Caspase-3 (Cleaved Asp175) Gene Symbol: CASP3 CPP32 Gene ID: 836 Accession #: P42574 ELISA Kit: RayBio Human/Mouse Cleaved-Caspase-3 (D175) ELISA	Caspase-7 (Cleaved Asp198) Gene Symbol: CASP7 MCH3 Gene ID: 840 Accession #: P55210
CHK1 (P-Ser296) Gene Symbol: CHEK1 CHK1 Gene ID: 1111 Accession #: O14757	CHK2 (P-Thr68) Gene Symbol: CHEK2 CDS1 CHK2 RAD53 Gene ID: 11200 Accession #: O96017 ELISA Kit: RayBio Human Phospho-Chk2 (T68) ELISA	eIF-2a (P-Ser52) Gene Symbol: EIF2A CDA02 MSTP004 MSTP089 Gene ID: 83939 Accession #: Q9BY44 ELISA Kit: RayBio Human Phospho-EIF2A (S52) ELISA	ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187) Gene Symbol: MAPK3 ERK1 PRKM3/MAPK1 ERK2 PRKM1 PRKM2 Gene ID: 5595/5594 Accession #: P27361/P28482 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (Y185/Y187) and Total Erk1/2 ELISA	HSP27 (P-Ser82) Gene Symbol: HSPB1 HSP27 HSP28 Gene ID: 3315 Accession #: P04792 ELISA Kit: RayBio Human Phospho-HSP27 (S82) ELISA
IKBa (P-Ser32) Gene Symbol: NFKBIA IKBA MAD3 NFKBI Gene ID: 4792 Accession #: P25963 ELISA Kit: RayBio Human Phospho-I-kappa B alpha (S32) ELISA	JNK (P-Thr183/Tyr185) Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C Gene ID: 5599 Accession #: P45983 ELISA Kit: RayBio Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA	NFKBP65 (P-Ser536) Gene Symbol: RELA NFKB3 Gene ID: 5970 Accession #: Q04206 ELISA Kit: RayBio Human Phospho-NF-KB p65 (S536) ELISA	PARP1 (Cleaved Asp214/Gly215) Gene Symbol: PARP1 ADPRT PPOL Gene ID: 142 Accession #: P09874 ELISA Kit: RayBio Human Cleaved-PARP (D214/G215) ELISA	p27 (P-Thr198) Gene Symbol: PSMD9 Gene ID: 5715 Accession #: O00233 ELISA Kit: RayBio Human Phospho-P27/Kip1 (T198) ELISA
P53 (P-Ser15) Gene Symbol: TP53 P53 Gene ID: 6198 Accession #: P23443 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA	SMAD2 (Ser245/250/255) Gene Symbol: SMAD2 MADH2 MADR2 Gene ID: 4087 Accession #: Q15796 ELISA Kit: RayBio Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) ELISA	TAK1 (P-Ser412) Gene Symbol: MAP3K7 TAK1 Gene ID: 6885 Accession #: O43318 ELISA Kit: RayBio Human Phospho-TAK1 (Ser412) ELISA

Typical Results

Figure 1: HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Staurosporine (STS) for 3 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Figure 2: Jurkat cells were either untreated (bottom panel) or treated (top panel) with Camptothecin(CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Apoptosis Signaling Antibody Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail
1,000X HRP-Anti-Rabbit IgG Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray w/ Lid
Protease Inhibitor Cocktail
100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.