Human Apoptosis Signaling Array C1

RayBio® C-Series Human Apoptosis Antibody Array 1 Kit. Detects 19 phosphorylated or cleaved human Apoptotic Factors. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

$414.04  
AAH-APOSIG-1-2
+

Antigen Information

Gene Symbols:
AKT1 | ATM | BAD | CASP3 | CASP7 | CHEK1 | CHEK2 | EIF2A | HSPB1 | MAP3K7   View more

Assay Format

Pathway:
  • Akt Signaling
  • HIF-1 alpha Signaling
  • IGF Signaling
  • JAK/STAT Signaling
  • MAPK Signaling
  • mTOR Signaling
  • NFkB Signaling
  • p53 Signaling
  • PI3K-AKT Signaling
  • TGF-beta Signaling
  • Wnt/beta-Catenin Signaling
Number of Targets Detected:
19
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473)
(PKB (P-Ser473))
ATM (P-Ser1981)
BAD (P-Ser112)
Caspase-3 (Cleaved Asp175)
Caspase-7 (Cleaved Asp198)
  • Gene Symbol: CASP7 MCH3
  • Gene ID: 840
  • Accession #: P55210
CHK1 (P-Ser296)
  • Gene Symbol: CHEK1 CHK1
  • Gene ID: 1111
  • Accession #: O14757
CHK2 (P-Thr68)
eIF-2a (P-Ser52)
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
HSP27 (P-Ser82)
IKBa (P-Ser32)
JNK (P-Thr183/Tyr185)
NFKBP65 (P-Ser536)
PARP1 (Cleaved Asp214/Gly215)
p27 (P-Thr198)
P53 (P-Ser15)
SMAD2 (Ser245/250/255)
TAK1 (P-Ser412)

Typical Results

Figure 1: HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Staurosporine (STS) for 3 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Figure 2: Jurkat cells were either untreated (bottom panel) or treated (top panel) with Camptothecin(CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Apoptosis Signaling Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated anti-IgG
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
The human apoptosis array provides a fast and comprehensive glance about the key signaling involved in the apoptotic pathway. It's a one shot experiment with certain controls already on the blot, pretty simple to execute and normalize. Would recommend it.

Moffitt Cancer Center
This apoptosis kit was very user-friendly and gave detailed instructions on how to analyze findings using a software like Excel. This assay was very helpful for examining multiple gene targets simultaneously, so we got a lot of data out of 1 experiment. Negative and positive controls were included on the blots, which was useful in subsequent analyses.

Notes: the Caspase3 antibody seemed a lot more sensitive than other antibodies on the blot. Also, some of the gene names in the kit manual differed from those listed on the website, so keep that in mind when analyzing data to ensure that the correct gene is being referred to.

Medical College of Wisconsin
It seems antibodies are not evenly attached on the membrane. Some signals show up outside of spots. It has high background when I follow up the suggested procedures. I cannot see any benefit of this array compared to other array such as apoptosis array or other company's similar product.

Penn State College of Medicine
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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