Human Amphiregulin ELISA

RayBio® Human Amphiregulin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.


Assay Format

Detection Range:
10 pg/ml - 4000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
10 pg/ml

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This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P15514
Gene ID:
  • 374
Gene Symbols:
  • AREG
  • SDGF
Protein Name & Synonyms:
Amphiregulin (AR) (Colorectum cell-derived growth factor) (CRDGF)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Lim Y., Yoo J., Kim M., et al. GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands. Mol Cancer Ther. 2016 Feb;15(2):251-63. doi: 10.1158/1535-7163.MCT-15-0679
    Species: Human
    Sample type: Conditioned Media (Tumor xenografts)
  2. Miyabayashi K, Ijichi H, Mohri D, et al. Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals. Cancer Res. 2013 Apr 1;73(7):2221-2234.
    Species: Human
    Sample type: Cell Lysate (pancreatic ductal carcinoma)
  3. Miyabayashi K, Ijichi H, Mohri D, et al. Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals. Cancer Res. 2013 Apr 1;73(7):2221-2234.
    Species: Human
    Sample type: (Human Pancreatic Ductal Adenocarcinoma Cell line (BxPC-3))
  4. Oliveras-Ferraros C., Cufi S., Queralt B., et al. Cross-suppression of EGFR ligands amphiregulin and epiregulin and de-repression of FGFR3 signalling contribute to cetuximab resistance in wild-type KRAS tumour cells. Br J Cancer. 2012 Apr 10;106(8):1406-14. doi: 10.1038/bjc.2012.103.
    Species: Human
    Sample type: Cell Lysate (A431 squamous cell carinoma cells)
  5. Morancho B., Martinez-Barriocanal A., Villanueva J., Arribas J. Role of ADAM17 in the non-cell autonomous effects of oncogene-induced senescence. Breast Cancer Res. 2015 Aug 12;17:106. doi: 10.1186/s13058-015-0619-7.
    Species: Human
    Sample type: Conditioned Media (MDA-MB-231, MCF-7 cells)
  6. Hirota, C. L., F. Moreau, V. Iablokov, M. Dicay, B. Renaux, M. D. Hollenberg, and W. K. Macnaughton. "Epidermal Growth Factor Receptor Transactivation Is Required for Proteinase-activated Receptor-2-induced COX-2 Expression in Intestinal Epithelial Cells." AJP: Gastrointestinal and Liver Physiology 303.1 (2012): n. pag. Web.
    Species: Human
    Sample type: Conditioned Media (CaCo-2 cells)
  7. Pei, Rongjuan, et al. "Hepatitis C virus infection induces the expression of amphiregulin, a factor related to the activation of cellular survival pathways and required for efficient viral assembly." Journal of general virology 92.10 (2011): 2237-2248.
    Species: Human
    Sample type: Conditioned Media (Huh7-Lunet cells)
  8. Hristozova, T., Konschak, R., Budach, V. and Tinhofer, I. (2012), A simple multicolor flow cytometry protocol for detection and molecular characterization of circulating tumor cells in epithelial cancers. Cytometry, 81A: 489-495. doi: 10.1002/cyto.a.22041
    Species: Human
    Sample type: Plasma (SCCHN Patients)

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Not for diagnostic or therapeutic use.

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