Human Autophagy Array C1

RayBio® C-Series Human Autophagy Array 1 Kit. Detects 20 Human Autophagic Factors. Suitable for all liquid sample types.

$348.45  
AAH-ATG-1-2
+

Antigen Information

Accession Number:
O60238 | O60934 | O75143 | O75582 | O94817 | O95166 | O95352 | P11279 | P37840 | Q13501   View more
Gene Symbols:
ATG10 | ATG12 | ATG13 | ATG3 | ATG4A | ATG4B | ATG5 | ATG7 | BECN1 | BNIP3L   View more

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

ATG12
  • Gene Symbol: ATG12 APG12 APG12L
  • Gene ID: 9140
  • Accession #: O94817
ATG7
  • Gene Symbol: ATG7 APG7L
  • Gene ID: 10533
  • Accession #: O95352
ATG10
  • Gene Symbol: ATG10 APG10L PP12616
  • Gene ID: 83734
  • Accession #: Q9H0Y0
ATG13
  • Gene Symbol: ATG13 KIAA0652
  • Gene ID: 9776
  • Accession #: O75143
ATG3
  • Gene Symbol: ATG3 APG3 APG3L
  • Gene ID: 64422
  • Accession #: Q9NT62
ATG4A
  • Gene Symbol: ATG4A APG4A AUTL2
  • Gene ID: 115201
  • Accession #: Q8WYN0
ATG4B
  • Gene Symbol: ATG4B APG4B AUTL1 KIAA0943
  • Gene ID: 23192
  • Accession #: Q9Y4P1
ATG5
  • Gene Symbol: ATG5 APG5L ASP
  • Gene ID: 9474
  • Accession #: Q9H1Y0
Beclin
  • Gene Symbol: BECN1 GT197
  • Gene ID: 8678
  • Accession #: Q14457
BNIP3L
  • Gene Symbol: BNIP3L BNIP3A BNIP3H NIX
  • Gene ID: 665
  • Accession #: O60238
DDR2
  • Gene Symbol: DDR2 NTRKR3 TKT TYRO10
  • Gene ID: 4921
  • Accession #: Q16832
GABARAP
  • Gene Symbol: GABARAP FLC3B HT004
  • Gene ID: 11337
  • Accession #: O95166
LAMP1
(CD107a)
LC3a
  • Gene Symbol: MAP1LC3A
  • Gene ID: 84557
  • Accession #: Q9H492
LC3B
  • Gene Symbol: MAP1LC3B MAP1ALC3
  • Gene ID: 81631
  • Accession #: Q9GZQ8
NBS1
  • Gene Symbol: NBN NBS NBS1 P95
  • Gene ID: 4683
  • Accession #: O60934
P62
  • Gene Symbol: SQSTM1 ORCA OSIL
  • Gene ID: 8878
  • Accession #: Q13501
RHEB
  • Gene Symbol: RHEB RHEB2
  • Gene ID: 6009
  • Accession #: Q15382
MSK1
  • Gene Symbol: RPS6KA5 MSK1
  • Gene ID: 9252
  • Accession #: O75582
Alpha-synuclein
  • Gene Symbol: SNCA NACP PARK1
  • Gene ID: 6622
  • Accession #: P37840

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Autophagy Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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