Human Axl ELISA

RayBio® Human AXL ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$402.00  
ELH-AXL-1
+

Assay Format

Detection Range:
2 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
20 - 200 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P30530
Gene ID:
  • 558
Gene Symbols:
  • AXL
  • UFO
Protein Name & Synonyms:
Tyrosine-protein kinase receptor UFO (EC 2.7.10.1) (AXL oncogene)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Hsiao F., Lin Y., Hsieh P., Chu N., Shieh Y., et al. Circulating Growth Arrest-Specific 6 Protein Is Associated With Adiposity, Systemic Inflammation, and Insulin Resistance Among Overweight and Obese Adolescents. JCEM Vol 98 Issue 2 http://dx.doi.org/10.1210/jc.2012-3179
    Species: Human
    Sample type: Plasma
  2. Hsiao F-C, Lin Y-F, Hsieh P-S, et al. Circulating growth arrest-specific 6 protein is associated with adiposity, systemic inflammation, and insulin resistance among overweight and obese adolescents. J Clin Endocrinol Metab. 2013;98(2):E267-E274.
    Species: Human
    Sample type: Plasma (intra-assay and interassay coefficients of variation were <10% and <12%)
  3. Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
    Species: Human
    Sample type: Serum (Lupus Nephritis patients)
  4. Farschtschi, S., Kluwe, L., Park, SJ. et al. Upregulated immuno-modulator PD-L1 in malignant peripheral nerve sheath tumors provides a potential biomarker and a therapeutic target. Cancer Immunol Immunother 69, 1307–1313 (2020). https://doi.org/10.1007/s00262-020-02548-1
    Species: Human
    Sample type: Serum
  5. Lee, Chien-Hsing, et al. "Plasma concentrations predict aortic expression of growth-arrest-specific protein 6 in patients undergoing coronary artery bypass grafting." PloS one 8.11 (2013): e79452.
    Species: Human
    Sample type: Plasma
  6. Liu YW., Yang QF., Zuo PY, et al. Elevated Serum Levels of Soluble Axl in Acute Coronary Syndrome. Am J Med Sci. 2015 Feb;349(2):124-9. doi: 10.1097/MAJ.0000000000000362
    Species: Human
    Sample type: Serum (Acute Coronary Syndrome patients)
  7. Bakr S., et al. Serum growth arrest specific protein 6 and its receptor (Axl) in SLE patients. The Egyptian Rheumatologist, Sept 2014.DOI:10.1016/j.ejr.2014.07.003
    Species: Human
    Sample type: Serum
This is a great kit. Was able to generate a very good standard curve, and the experiment itself worked

Plymouth University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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