Human BDNF ELISA

RayBio® Human BDNF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELH-BDNF-1
+

Antigen Information

Accession Number:
  • P23560
Gene ID:
  • 627
Gene Symbols:
  • BDNF
Protein Name & Synonyms:
Brain-derived neurotrophic factor (BDNF) (Abrineurin)
Target Species:
  • Human

Assay Format

Detection Range:
80 pg/ml - 16 ng/ml
Recommended Dilution (serum & plasma):
5 - 500 fold
Sensitivity:
80 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN- gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF- beta, TIMP-1, TIMP-2, TNF- alpha, TNF- beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Liu Y., et al. Associations Between ApoE?4 Carrier Status and Serum BDNFLevels—New Insights into the Molecular Mechanism of ApoE?4. Mol Neurobio. Online First June 2014. DOI 10.1007/s12035-014-8804-8Actions in Alzheimer’s Disease
    Species: Human
    Sample type: Serum
  2. Sahin S., et al. Effect of Methylphenidate Treatment on Appetite and Levels of Leptin, Ghrelin, Adiponectin, and Brain-Derived Neurotrophic Factor in Children and Adolescents with Attention Deficit and Hyperactivity Disorder. International Journal of Pyschiatry in Clinical Practice. Posted online on July 4, 2014. (doi:10.3109/13651501.2014.940054)
    Species: Human
    Sample type: Serum
  3. Sinkevicius K., et al. Neurotrophin receptor TrkB promotes lungadenocarcinoma metastasis. PNAS 2014 111 (28) 10299-10304; published ahead of print June 30, 2014, doi:10.1073/pnas.1404399111
    Species: Human
    Sample type: Conditioned Media
  4. Oral E, Ozcan H, Kirkan TS, et al. Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder. Eur Arch Psychiatry Clin Neurosci. 2013 Mar 2; [Epub ahead of print] doi:10.1007/s00406-013-0398-z.
    Species: Human
    Sample type: Serum
  5. Pompeia S, Gouveia JR, Galduróz JCF. Acute mood effect of donepezil in young, healthy volunteers. Hum Psychopharmacol. 2013 May;28(3):263-269.
    Species: Human
    Sample type: Plasma
  6. Uguz F, Sonmez EO, Sahingoz M, et al. Maternal generalized anxiety disorder during pregnancy and fetal brain development: A comparative study on cord blood brain-derived neurotrophic factor levels. J Psychosomatic Res. 2013 Aug 5; [Epub ahead of print] doi:10.1016/j.jpsychores.2013.07.002.
    Species: Human
    Sample type: Serum
  7. Narbutt, Joanna, et al. "Narrow band ultraviolet B irradiations cause alteration in interleukin-31 serum level in psoriatic patients." Archives of dermatological research (2013): 1-5.
    Species: Human
    Sample type: Serum
  8. Dudás, József, et al. "Curcumin targets fibroblast–tumor cell interactions in oral squamous cell carcinoma." Experimental cell research 319.6 (2013): 800-809.
    Species: Human
    Sample type: Conditioned Media
  9. prep tips we pri
    Species: Human
    Sample type: Tissue Lysate
  10. Oral E., Canpolat S., Yildiirim S., et al. Cognitive functions and serum levels of brain-derived neurotrophic factor in patients with major depressive disorder. Brain Res Bull. 2012 Aug 1;88(5):454-9. doi: 10.1016/j.brainresbull.2012.03.005.
    Species: Human
    Sample type: Serum
Helped teach the lab assistants how to use it, worked out well. User-friendly and accurate.

UNC
The BDNF ELISA kit was very easy to work with. I had no problems understanding the directions and following them. I used the kit once it arrived and received great results.

UNCO
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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