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Human BDNF ELISA

The RayBiotech BDNF ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BDNF in serum, plasma, and cell culture supernatant samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$402.00  
ELH-BDNF-1
+

Assay Format

Detection Range:
80 pg/ml - 16 ng/ml
Recommended Dilution (serum & plasma):
5 - 500 fold
Sensitivity:
80 pg/ml
Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN- gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF- beta, TIMP-1, TIMP-2, TNF- alpha, TNF- beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P23560
Gene ID:
  • 627
Gene Symbols:
  • BDNF
Protein Name & Synonyms:
BDNF
Target Species:
  • Human

Introduction

The BDNF ELISA employs an antibody specific for human BDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and BDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BDNF antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Brain-derived neurotrophic factor (BDNF) is found in neurons of the central nervous system and is a member of the nerve growth factor family of proteins. It is expressed predominantly in hippocampus, cortex, and synapses of the basal forebrain. BDNF selectively supports the survival of primary sensory neurons and retinal ganglia. It supports survival and differentiation of certain cholinergic neurons and also some dopaminergic neurons in vitro. The factor inhibits the normal cell death of embryonic chick motor neurons. Expression of the BDNF gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. It may play a role in the regulation of the stress response and in the biology of mood disorders.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

ELISA Kit Standard Curve Figures

Spiking & Recovery Results

Sample Type Average % Recovery Range (%)
Serum 94.57 83-103
Plasma 92.48 84-102
Cell culture media 93.35 83-102

Linearity Results

    Serum Plasma Cell Culture Media
1:2 Average % of expected
Range (%)
93
83-103
92
84-103
94
83-104
1:4 Average % of expected
Range (%)
94
84-104
93
83-102
95
85-103

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Wang X., Prager B., Wu Q., et al. Reciprocal Signaling between Glioblastoma Stem Cells and Differentiated Tumor Cells Promotes Malignant Progression. Cell Stem Cell . 2018 Apr 5;22(4):514-528.e5. doi: 10.1016/j.stem.2018.03.011.
    Species: Human
    Sample type: Conditioned Media (Glioblastoma stem cells and differentiated cells)
  2. Sinkevicius K., et al. Neurotrophin receptor TrkB promotes lungadenocarcinoma metastasis. PNAS 2014 111 (28) 10299-10304; published ahead of print June 30, 2014, doi:10.1073/pnas.1404399111
    Species: Human
    Sample type: Conditioned Media
  3. prep tips we pri
    Species: Human
    Sample type: Tissue Lysate (Endometrium tissue)
  4. Jiang H., Ling Z., Zhang Y., et al. Altered fecal microbiota composition in patients with major depressive disorder. Brain Behav Immun. 2015 Aug;48:186-94. doi: 10.1016/j.bbi.2015.03.016
    Species: Human
    Sample type: Serum (Major Depressive Disorder Patients)
  5. Namgyal D, Chandan K, Sultan A, Aftab M, Ali S, Mehta R, El-Serehy HA, Al-Misned FA, Sarwat M. Dim Light at Night Induced Neurodegeneration and Ameliorative Effect of Curcumin. Cells. 2020 Sep 13;9(9):2093. doi: 10.3390/cells9092093. PMID: 32933226; PMCID: PMC7565558.
    Species: Mouse
    Sample type: Tissue Lysate (Hippocampus)
  6. Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 - 1883
    Species: Human
    Sample type: Serum
  7. Liu Y., et al. Associations Between ApoE?4 Carrier Status and Serum BDNFLevels—New Insights into the Molecular Mechanism of ApoE?4. Mol Neurobio. Online First June 2014. DOI 10.1007/s12035-014-8804-8Actions in Alzheimer’s Disease
    Species: Human
    Sample type: Serum (Alzheimer's Patients)
  8. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.
    Species: Human
    Sample type: Conditioned Media (Vertebral disc conditioned media)
  9. Failla M., Conley Y., Wagner A. Brain-Derived Neurotrophic Factor (BDNF) in Traumatic Brain Injury-Related Mortality: Interrelationships Between Genetics and Acute Systemic and Central Nervous System BDNF Profiles. Neurorehabil Neural Repair. 2016 Jan;30(1):83-93. doi: 10.1177/1545968315586465.
    Species: Human
    Sample type: Serum (BDNF in traumatic Brain Injury mortality)
  10. Failla M., Conley Y., Wagner A. Brain-Derived Neurotrophic Factor (BDNF) in Traumatic Brain Injury-Related Mortality: Interrelationships Between Genetics and Acute Systemic and Central Nervous System BDNF Profiles. Neurorehabil Neural Repair. 2016 Jan;30(1):83-93. doi: 10.1177/1545968315586465.
    Species: Human
    Sample type: CSF (BDNF in traumatic Brain Injury mortality)
Amazing kit! Easy to use, reliable, convenient. Totally recommended.

NIA/NIH
Two kits were purchased during a promotional sale, and were just used this week. The standard curve was perfect as compared to QC data. Although the samples were saturated (my fault with dilution), the kit worked properly! Looking forward to ordering again for our sample databases.

University of Northern Colorado
Great directions and easy to use.

UNC
The BDNF ELISA kit was easy to use and produced accurate results. The instructions were clear and the protocol was straight forward. I ran a series of 4 plates and did not have any issues.

University of Arkansas
Helped teach the lab assistants how to use it, worked out well. User-friendly and accurate.
The BDNF ELISA kit was very easy to work with. I had no problems understanding the directions and following them. I used the kit once it arrived and received great results.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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