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Human BDNF ELISA

RayBio® Human BDNF ELISA Kit for cell culture supernatants, plasma, and serum samples.

$402.00  
ELH-BDNF-1
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Antigen Information

Accession Number:
  • P23560
Gene ID:
  • 627
Gene Symbols:
  • BDNF
Protein Name & Synonyms:
BDNF
Target Species:
  • Human

Assay Format

Detection Range:
80 pg/ml - 16 ng/ml
Recommended Dilution (serum & plasma):
5 - 500 fold
Sensitivity:
80 pg/ml
Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN- gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF- beta, TIMP-1, TIMP-2, TNF- alpha, TNF- beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Failla M., Juengst S., Arenth P., Wagner A. Preliminary Associations Between Brain-Derived Neurotrophic Factor, Memory Impairment, Functional Cognition, and Depressive Symptoms Following Severe TBI. Neurorehabil Neural Repair. 2016 Jun;30(5):419-30. doi: 10.1177/1545968315600525.
    Species: Human
    Sample type: CSF
  2. Cadenas-Sánchez, Cristina, et al. "An exercise-based randomized controlled trial on brain, cognition, physical health and mental health in overweight/obese children (ActiveBrains project): Rationale, design and methods." Contemporary clinical trials 47 (2016): 315-324.
    Species: Human
    Sample type: Serum
  3. van den Heuvel L., Suliman S., Malan-Muller S., et al. Brain-derived neurotrophic factor Val66met polymorphism and plasma levels in road traffic accident survivors. Anxiety Stress Coping. 2016 Nov;29(6):616-29. doi: 10.1080/10615806.2016.1163545.
    Species: Human
    Sample type: Plasma
  4. Sadowska-Krepa E., Zwierzchowska A., Glowacz M., et al. Blood metabolic response to a long-term wheelchair rugby training. Spinal Cord. 2016 May;54(5):371-5. doi: 10.1038/sc.2015.178.
    Species: Human
    Sample type: Serum
  5. Wysokinski A., Serum levels of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in depressed patients with schizophrenia. Nord J Psychiatry. 2016;70(4):267-71. doi: 10.3109/08039488.2015.1087592
    Species: Human
    Sample type: Serum
  6. Jiang H., Ling Z., Zhang Y., et al. Altered fecal microbiota composition in patients with major depressive disorder. Brain Behav Immun. 2015 Aug;48:186-94. doi: 10.1016/j.bbi.2015.03.016
    Species: Human
    Sample type: Serum
  7. Failla M., Conley Y., Wagner A. Brain-Derived Neurotrophic Factor (BDNF) in Traumatic Brain Injury-Related Mortality: Interrelationships Between Genetics and Acute Systemic and Central Nervous System BDNF Profiles. Neurorehabil Neural Repair. 2016 Jan;30(1):83-93. doi: 10.1177/1545968315586465.
    Species: Human
    Sample type: CSF
  8. Faroni A., Smith R., Lu L., et al. Human Schwann-like cells derived from adipose-derived mesenchymal stem cells rapidly de-differentiate in the absence of stimulating medium. Eur J Neurosci. 2016 Feb;43(3):417-30. doi: 10.1111/ejn.13055.
    Species: Human
    Sample type: Conditioned Media
  9. Kielstein H., Suntharalingam M., Perthel R., et al. Role of the endogenous nitric oxide inhibitor asymmetric dimethylarginine (ADMA) and brain-derived neurotrophic factor (BDNF) in depression and behavioural changes: clinical and preclinical data in chronic kidney disease. Nephrol Dial Transplant. 2015 Oct;30(10):1699-705. doi: 10.1093/ndt/gfv253
    Species: Human
    Sample type: Serum
  10. Eleftheriou D., Ganesan V., Hong Y., et al. Endothelial Repair in Childhood Arterial Ischaemic Stroke with Cerebral Arteriopathy. Cerebrovasc Dis Extra. 2015 Jun 11;5(2):68-74. doi: 10.1159/000381963.
    Species: Human
    Sample type: Plasma
Helped teach the lab assistants how to use it, worked out well. User-friendly and accurate.
The BDNF ELISA kit was very easy to work with. I had no problems understanding the directions and following them. I used the kit once it arrived and received great results.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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