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CODE | Number of Plates | Price | ||
---|---|---|---|---|
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ELH-BDNF-1
|
$420.00 | ||
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ELH-BDNF-5
|
$1,680.00 | ||
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ELH-BDNF-2
|
$756.00 |
The BDNF ELISA employs an antibody specific for human BDNF coated on a 96-well plate. Standards and samples are pipetted into the wells and BDNF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BDNF antibody is added. After washing away unbound biotinylated antibody, HRPconjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BDNF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Brain-derived neurotrophic factor (BDNF) is found in neurons of the central nervous system and is a member of the nerve growth factor family of proteins. It is expressed predominantly in hippocampus, cortex, and synapses of the basal forebrain. BDNF selectively supports the survival of primary sensory neurons and retinal ganglia. It supports survival and differentiation of certain cholinergic neurons and also some dopaminergic neurons in vitro. The factor inhibits the normal cell death of embryonic chick motor neurons. Expression of the BDNF gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. It may play a role in the regulation of the stress response and in the biology of mood disorders.
Sample Type | Average % Recovery | Range (%) |
---|---|---|
Serum | 94.57 | 83-103 |
Plasma | 92.48 | 84-102 |
Cell culture media | 93.35 | 83-102 |
Serum | Plasma | Cell Culture Media | ||
---|---|---|---|---|
1:2 | Average % of expected Range (%) |
93 83-103 |
92 84-103 |
94 83-104 |
1:4 | Average % of expected Range (%) |
94 84-104 |
93 83-102 |
95 85-103 |
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.