Human bFGF ELISA

RayBio® Human bFGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-bFGF-1
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Antigen Information

Accession Number:
  • P09038
Gene ID:
  • 2247
Gene Symbols:
  • FGF2
  • FGFB
Protein Name & Synonyms:
Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Target Species:
  • Human

Assay Format

Detection Range:
50 pg/ml - 10000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
50 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Synergistic effect of vascular endothelial growth factor and angiopoietin-2 on progression free survival in multiple myeloma
    Species: Human
    Sample type: Serum
  2. Yalç?nda? A, Gedik-O?uz Y, Yalç?nda? FN. The relationship between serum levels of angiogenin, bFGF, VEGF, and ocular involvement in patients with Behçet’s disease. Graefes Arch. Clin. Exp. Ophthalmol. 2013 Apr 4;
    Species: Human
    Sample type: Serum
  3. Gok, Mahmut, et al. "Relationship of ultrasonographic findings with synovial angiogenesis modulators in different forms of knee arthritides." Rheumatology international 33.4 (2013): 879-885.
    Species: Human
    Sample type: Synovial Fluid
  4. Bhaskar, Archana, et al. "Angiopoietins as biomarker of disease activity and response to therapy in multiple myeloma." Leukemia & lymphoma 54.7 (2013): 1473-1478.
    Species: Human
    Sample type: Serum
  5. Chang, Chien-Wen, et al. "Engineering cell–material interfaces for long-term expansion of human pluripotent stem cells." Biomaterials 34.4 (2013): 912-921.
    Species: Human
    Sample type: Conditioned Media
  6. Esmaeelinejad, M., & Bayat, M. (2013). Effect of low-level laser therapy on the release of interleukin-6 and basic fibroblast growth factor from cultured human skin fibroblasts in normal and high glucose mediums. Journal of Cosmetic and Laser Therapy, 15(6), 310-317.
    Species: Human
    Sample type: Conditioned Media
  7. Koob, Thomas J., et al. "Biological properties of dehydrated human amnion/chorion composite graft: implications for chronic wound healing." International wound journal 10.5 (2013): 493-500.
    Species: Human
    Sample type: Tissue Lysate
  8. Sugihara, Kazuhiro, et al. "Attenuation of fibroblast growth factor signaling by poly?N?acetyllactosamine type glycans." FEBS letters 587.19 (2013): 3195-3201.
    Species: Human
    Sample type: Cell Lysate
  9. Chim H., Armijo BS., Miller E., et al. Propranolol induces regression of hemangioma cells through HIF-1?-mediated inhibition of VEGF-A. Ann Surg. 2012 Jul;256(1):146-56. doi: 10.1097/SLA.0b013e318254ce7a.
    Species: Human
    Sample type: Conditioned Media
  10. Fedak PW, Bai L., Turnbull J., et al. Cell therapy limits myofibroblast differentiation and structural cardiac remodeling: basic fibroblast growth factor-mediated paracrine mechanism. Circ Heart Fail. 2012 May 1;5(3):349-56. doi: 10.1161/CIRCHEARTFAILURE.111.965889
    Species: Human
    Sample type: Tissue Lysate
The ELISA instructions are concise and easy to follow. The kit also has all reagents you need. It has great reproducibility and reliability.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.