Human bFGF ELISA

RayBio® Human bFGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELH-bFGF-1
+

Antigen Information

Accession Number:
  • P09038
Gene ID:
  • 2247
Gene Symbols:
  • FGF2
  • FGFB
Protein Name & Synonyms:
Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Target Species:
  • Human

Assay Format

Detection Range:
50 pg/ml - 10000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
50 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Kuhn MN., Willenberg HS., Schott M., et al. Adipocyte-secreted factors increase osteoblast proliferation and the OPG/RANKL ratio to influence osteoclast formation. Mol Cell Endocrinol. 2012 Feb 26;349(2):180-8. doi: 10.1016/j.mce.2011.10.018.
    Species: Human
    Sample type: Conditioned Media
  2. Nazarov I, Lee JW, Soupene E, et al. Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential. Stem Cells Translational Medicine. 2012;1(5):359-372. doi:10.5966/sctm.2011-0021.
    Species: Human
    Sample type: Conditioned Media
  3. Brown K., Lau J., Dom A., et al. MG624, an ?7-nAChR antagonist, inhibits angiogenesis via the Egr-1/FGF2 pathway. Angiogenesis. 2012 Mar;15(1):99-114. doi: 10.1007/s10456-011-9246-9.
    Species: Human
    Sample type: Conditioned Media
  4. Saygun I., Nizam N., Ural A., et al. Low-level laser irradiation affects the release of basic fibroblast growth factor (bFGF), insulin-like growth factor-I (IGF-I), and receptor of IGF-I (IGFBP3) from osteoblasts. Photomed Laser Surg. 2012 Mar;30(3):149-54. doi: 10.1089/pho.2011.3079.
    Species: Human
    Sample type: Conditioned Media
  5. Dragoo J., Korotkova T., Wasterlain A., et al. Age-Related Changes of Chondrogenic Growth Factors in Platelet-Rich Plasma. Operative Techniques in Orthopaedics, Volume 22, Issue 2, June 2012, Pages 49-55
    Species: Human
    Sample type: Plasma
  6. Yamamoto, Masaki, et al. "The acceleration of wound healing in primates by the local administration of immunostimulatory CpG oligonucleotides." Biomaterials 32.18 (2011): 4238-4242.
    Species: Human
    Sample type: Conditioned Media
  7. Rose, Karsten. "Interaction of ATP with fibroblast growth factor 2: biochemical characterization and consequence for growth factor stability." BMC biochemistry 12.1 (2011): 14.
    Species: Human
    Sample type: Conditioned Media
  8. Uzar, Ertu?rul, et al. "?ntraserebral kanamal? hastalarda serum fibroblast büyüme faktörü düzeyleri." Journal of Clinical and Experimental Investigations 2.3 (2011).
    Species: Human
    Sample type: Serum
  9. Go, Dewi P., et al. "Multilayered microspheres for the controlled release of growth factors in tissue engineering." Biomacromolecules 12.5 (2011): 1494-1503.
    Species: Human
    Sample type: Conditioned Media
The ELISA instructions are concise and easy to follow. The kit also has all reagents you need. It has great reproducibility and reliability.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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