Human bFGF ELISA

RayBio® Human bFGF ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$1,680.00  
ELH-bFGF-CL-5
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Assay Format

Detection Range:
50 pg/ml - 10000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
50 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P09038
Gene ID:
  • 2247
Gene Symbols:
  • FGF2
  • FGFB
Protein Name & Synonyms:
Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Target Species:
  • Human

Target Background

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, is a member of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (HST/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Yamamoto, Masaki, et al. "The acceleration of wound healing in primates by the local administration of immunostimulatory CpG oligonucleotides." Biomaterials 32.18 (2011): 4238-4242.
    Species: Human
    Sample type: Conditioned Media
  2. Chang, Chien-Wen, et al. "Engineering cell–material interfaces for long-term expansion of human pluripotent stem cells." Biomaterials 34.4 (2013): 912-921.
    Species: Human
    Sample type: Conditioned Media (PAm6-co-A2AGA2 and PAm6-co-PSS2 hydrogels)
  3. Chim H., Armijo BS., Miller E., et al. Propranolol induces regression of hemangioma cells through HIF-1?-mediated inhibition of VEGF-A. Ann Surg. 2012 Jul;256(1):146-56. doi: 10.1097/SLA.0b013e318254ce7a.
    Species: Human
    Sample type: Conditioned Media (Hemangioma endothelial cells treated with propranolol)
  4. Fedak PW, Bai L., Turnbull J., et al. Cell therapy limits myofibroblast differentiation and structural cardiac remodeling: basic fibroblast growth factor-mediated paracrine mechanism. Circ Heart Fail. 2012 May 1;5(3):349-56. doi: 10.1161/CIRCHEARTFAILURE.111.965889
    Species: Human
    Sample type: Tissue Lysate (Vascular Smooth Muscle Cell Lysates)
  5. Lai H., Kuan C., Wu H., Tsai J., Chen T., Hsieh D., Wang T. Tailor Design Electrospun Composite Nanofibers with Staged Release of Multiple Angiogenic Growth Factors for Chronic Wound Healing. Acta Biomaterialia Available online May 9, 2014. http://dx.doi.org.proxy.library.emory.edu/10.1016/j.actbio.2014.05.001 [Epub ahead of print]
    Species: Human
    Sample type: Nanomaterials (Nanomaterials incorporation of bFGF)
  6. Go, Dewi P., et al. "Multilayered microspheres for the controlled release of growth factors in tissue engineering." Biomacromolecules 12.5 (2011): 1494-1503.
    Species: Human
    Sample type: Conditioned Media (release from PLGA engineered tissue)
  7. Nazarov I, Lee JW, Soupene E, et al. Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential. Stem Cells Translational Medicine. 2012;1(5):359-372. doi:10.5966/sctm.2011-0021.
    Species: Human
    Sample type: Conditioned Media ( human chorionic mesenchymal stem cell derived from placenta MSCs derived from human bone marrow)
  8. Guan P., Guo J., Yu X., et al. The role of cyclooxygenase-2, interleukin-1? and fibroblast growth factor-2 in the activation of matrix metalloproteinase-1 in sheared-chondrocytes and articular cartilage. Sci Rep. 2015 May 20;5:10412. doi: 10.1038/srep10412.
    Species: Human
    Sample type: Conditioned Media (Human Chondrocytes)
  9. Kumar A, Jovel J, Lopez-Orozco J, Limonta D, Airo AM, Hou S, Stryapunina I, Fibke C, Moore RB, Hobman TC. Human Sertoli cells support high levels of Zika virus replication and persistence. Sci Rep. 2018 Apr 3;8(1):5477. doi: 10.1038/s41598-018-23899-x. PMID: 29615760; PMCID: PMC5883016.
    Species: Human
    Sample type: Conditioned Media
  10. Brown K., Lau J., Dom A., et al. MG624, an ?7-nAChR antagonist, inhibits angiogenesis via the Egr-1/FGF2 pathway. Angiogenesis. 2012 Mar;15(1):99-114. doi: 10.1007/s10456-011-9246-9.
    Species: Human
    Sample type: Conditioned Media (Human Microvascular Endothelial Cells of the Lugn Culture Sup)
Easy to use and allowed to run different conditions

University of Puerto Rico
Easy to follow protocol with good performance and reproducibility.
Reagents must be stored and used following vendor recommendations better results.

University of Puerto Rico
I used the cell culture supernatant, sometimes, you need to work on different dilution factor

Florida A&M University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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