RayBio® Human Brain Natriuretic Peptide EIA Kit optimized for serum and cell culture medium. Competition-based ELISA on a 96-well strip plate.


Assay Format

Detection Range:
0.1-1,000 pg/ml
Recommended Dilution (serum & plasma):
Human: 2X
1.66 pg/ml
Cross Reactivity: This EIA kit shows no cross-reactivity with any of the adipokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Serum
Design Principle:
  • Competition-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P16860
Gene ID:
  • 4879
Gene Symbols:
  • NPPB
Protein Name & Synonyms:
Natriuretic peptides B (Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 32 (BNP(1-32)) (BNP-32) BNP(1-30) BNP(1-29) BNP(1-28) BNP(2-31) BNP(3-32) BNP(3-30) BNP(3-29) BNP(4-32) BNP(4-31) BNP(4-30) BNP(4-29) BNP(4-27) BNP(5-32) BNP(5-31) BNP(5-29)]


Brain natriuretic peptide (BNP), (aka B-type natriuretic peptide), is a 32 amino acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of myocytes in the ventricles. BNP was originally identified in extracts of porcine brain, but in humans it is produced mainly in the cardiac ventricles. Its counterpart in rats is a 45 amino acid peptide hormone. At the time of release, a co-secreted 76 amino acid N-terminal fragment (NT-proBNP) is also released with BNP.

BNP binds to and activates NPRA in a similar fashion to atrial natriuretic peptide (ANP) but with 10-fold lower affinity. The biological half-life of BNP, however, is twice as long as that of ANP. Both ANP and BNP have limited ability to bind and activate NPRB.

Physiologic actions of BNP include decrease in systemic vascular resistance and central venous pressure as well as an increase in natriuresis. Thus, the resulting effect of BNP is a decrease in cardiac output and a decrease in blood volume.

Tests showing elevated levels of BNP or NT-proBNP in blood are used as a diagnosis of heart failure and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with poorer outcome.

Both BNP and NT-proBNP have been approved as a marker for acute congestive heart failure (CHF). The plasma concentrations of both BNP are increased in patients with asymptomatic and symptomatic left ventricular dysfunction. There is no level of BNP that perfectly separates patients with and without heart failure.

BNP EIA/ELISA Test Kit Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Application Notes

  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
  • Prepare all reagents, samples and standards as instructed.
  • Add 100 µl detection antibody to each well.
  • Incubate 1.5 h at RT or O/N at 4°C.
  • Add 100 µl standard or sample to each well.
  • Incubate 2.5 h at RT.
  • Add 100 µl prepared streptavidin solution.
  • Incubate 45 min at RT.
  • Add 100 µl TMB One-Step Substrate Reagent to each well.
  • Incubate 30 min at RT.
  • Add 50 µl Stop Solution to each well.
  • Read plate at 450 nm immediately.


Standard, Biotinylated Brain Natriuretic peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
  1. Sakaguchi T., Takefuji M., Wettschureck N., et al. Protein Kinase N Promotes Stress-Induced Cardiac Dysfunction Through Phosphorylation of Myocardin-Related Transcription Factor A and Disruption of Its Interaction With Actin. Circulation . 2019 Nov 19;140(21):1737-1752. doi: 10.1161/CIRCULATIONAHA.119.041019.
    Species: Mouse
    Sample type: Plasma
  2. Tang B., Chen GX., Liang MY., et al. Ellagic acid prevents monocrotaline-induced pulmonary artery hypertension via inhibiting NLRP3 inflammasome activation in rats. International Journal of Cardiology 180 (2015) 134-141
    Species: Rat
    Sample type: Serum
  3. Li Y., Xuan Y., Liu S., et al. Short?term vagal nerve stimulation improves left ventricular function following chronic heart failure in rats. Mol Med Rep. 2015 Aug;12(2):1709-16. doi: 10.3892/mmr.2015.3597.
    Species: Rat
    Sample type: Serum
  4. Kazakov A., Meier T., Werner C., et al. C-kit(+) resident cardiac stem cells improve left ventricular fibrosis in pressure overload. Stem Cell Res. 2015 Nov;15(3):700-11. doi: 10.1016/j.scr.2015.10.017
    Species: Mouse
    Sample type: Serum
  5. Jara A., et al. Elevated Systolic Blood Pressure in Male GH Transgenic Mice Is Age Dependent. Endocrinology, March 2014, 155(3):975-986. doi: 10.1210/en.2013-1899
    Species: Mouse
    Sample type: Serum
  6. Liu L., Aquirre SA., Evering WE., Hirakawa BP., May JR., Palacio K., Wang J., Zhang Y., Stevens GJ. mir-208a as a Biomarker of isoproterenol-induced Cardiac Injury in Sod 2+/- and C57BL/6 Wild-Type Mice. Toxicol Pathol. 2014 Apr 8. [Epub ahead of print]
    Species: Mouse
    Sample type: Plasma
  7. Martin, R., Cordova C., San Roman JA., Gutierrez B., Cachofeiro V., Nieto ML. Oleanolic acid Modulates the Immune-Inflammatory Response in Mice with Experimental Autoimmune Myocarditis and Protects from Cardiac Injury. J Mol Cell Cardiol. 2014 Apr 13;72C:250-262
    Species: Mouse
    Sample type: Serum
  8. Shah L., Kulkarni P., Ferris C., Amiji M. Analgesic Efficacy and Safety of DALDA Peptide Analog Delivery to the Brain Using Oil-in-Water Nanoemulsion Formulation. Pharmaceutical Research, May 2014 DOI 10.1007/s11095-014-1370-y [Epub ahead of print]
    Species: Mouse
    Sample type: Plasma
  9. Aurich AC., et al. Age-dependent effects of high fat-diet on murine left ventricles: role of palmitate. Basic Res Cardiol (2013) 108:369. doi:10.1007/s00395-013-0369-6
    Species: Mouse
    Sample type: Plasma
  10. Bordicchia M., et al. Cardiac natriuretic peptides act via p38 MAPK to induce the brown fat thermogenic program in mouse and human adipocytes. J Clin Invest. Mar 1, 2012; 122(3): 1022-1036.
    Species: Mouse
    Sample type: Plasma

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