Human CRP ELISA

RayBio® Human CRP (C-Reactive Protein) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$361.80
You save: $40.20 (10%)
ELH-CRP-1
+

Antigen Information

Accession Number:
  • P02741
Gene ID:
  • 1401
Gene Symbols:
  • CRP
  • PTX1
Protein Name & Synonyms:
C-reactive protein [Cleaved into: C-reactive protein(1-205)]
Target Species:
  • Human

Assay Format

Detection Range:
34 pg/ml - 25,000 pg/ml
Recommended Dilution (serum & plasma):
20,000 fold
Sensitivity:
34 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GM-CSF, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Zhang L., Wang X., Miao Y., et al. Magnetic ferroferric oxide nanoparticles induce vascular endothelial cell dysfunction and inflammation by disturbing autophagy. J Hazard Mater. 2016 Mar 5;304:186-95. doi: 10.1016/j.jhazmat.2015.10.041
    Species: Human
    Sample type: Conditioned Media
  2. Priya P., Rajappa M., Kattimani S., et al. Association of neurotrophins, inflammation and stress with suicide risk in young adults. Clin Chim Acta. 2016 Jun 1;457:41-5. doi: 10.1016/j.cca.2016.03.019.
    Species: Human
    Sample type: Serum
  3. Qian Y., Zhang S., Guo W., et al. Polychlorinated biphenyls (PCBs) inhibit hepcidin expression through an estrogen-like effect associated with disordered systemic iron homeostasis. Chem Res Toxicol. 2015 Apr 20;28(4):629-40. doi: 10.1021/tx500428r
    Species: Human
    Sample type: Conditioned Media
  4. Chakraborty A., Ghosh S., Dutta A., et al. Study the association of adiponectin with inflammation and hypercoagulability in case of type 2 diabetic subjects with renal dysfunction. Chakraborty et al., Cogent Medicine (2015), 2: 1015224 http://dx.doi.org/10.1080/2331205X.2015.1015224
    Species: Human
    Sample type: Plasma
  5. Ciaffi L., Cavassini M., Genne D., et al. Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study. Eur J Clin Invest. 2015 Jul;45(7):720-30. doi: 10.1111/eci.12464.
    Species: Human
    Sample type: Serum
  6. Verna E., Patel J., Bettencourt R., et al. Novel association between serum pentraxin-2 levels and advanced fibrosis in well-characterised patients with non-alcoholic fatty liver disease. Aliment Pharmacol Ther. 2015 Sep;42(5):582-90. doi: 10.1111/apt.13292.
    Species: Human
    Sample type: Serum
  7. Wadood S., Saihood Y., Mohammed Z. Determination of Angiopoietin Like Protein-4 Levels and Some Parameters in Iraqi Patients with Type 2 Diabetes Mellitus. Journal of Al-Nahrain University, Vol.18 (2), June, 2015, pp.33-43
    Species: Human
    Sample type: Plasma
  8. Zemer-Wassercug N., Haim M., Leshem-Lev D., et al. The effect of dabigatran and rivaroxaban on platelet reactivity and inflammatory markers. J Thromb Thrombolysis. 2015 Oct;40(3):340-6. doi: 10.1007/s11239-015-1245-z.
    Species: Human
    Sample type: Plasma
  9. Wadood S., Shawk R., Hashem R. Variants of MMP-9 and TIMP-1 Levels Could be a Predictor of an Early Development of Cardiovascular Diseases in Type 2 Diabetes among Iraqi Patients. Iraqi Journal of Science, 2015, Vol 56, No.1C, pp:622-632
    Species: Human
    Sample type: Serum
  10. Wang W., Song X., Chen Y., et al. The Long-Term Influence of Tissue Inhibitor of Matrix Metalloproteinase-1 in Patients with Mild to Moderate Coronary Artery Lesions in a Chinese Population: A 7-Year Follow-Up Study. Cardiology. 2015;132(3):151-8. doi: 10.1159/000430885
    Species: Human
    Sample type: Plasma

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Amazon Gift Card Program

Customers Also Bought Customers Also Bought