770-729-2992

Human Cell Cycle Array L1, Glass Slide

Label-based antibody array kit using glass slide supports to detect 89 human proteins involved in cell cycle pathways. Suitable for serum, plasma, lysates, and cell culture supernatants.

$423.00  
AAH-BLG-CYC-4
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Assay Format

Number of Targets Detected:
89
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Label-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Protein Information

Gene Symbols:
ABL1 | ANAPC1 | ANAPC13 | ANAPC2 | ANAPC4 | ATM | ATR | BUB1 | BUB3 | CCNA1   View more

Product Features

  • High density arrays
  • Unbiased detection through direct biotin labeling of samples
  • Low sample consumption
  • High detection sensitivity
  • Same day result
  • Affordable and simple to use

Target Names

14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, ABL1, ANAPC1, ANAPC13, ANAPC2, ANAPC4, ATM, ATR, BUB1, BUB3, CBP, CCNA1, CCNA2, CCNB2, CCND2, CCND3, CCNE1, CCNE2, CDC14A, CDC14B, CDC16, CDC2, CDC23, CDC25A, CDC25B, CDC25C, CDC26, CDC27, CDC6, CDK2, CDK4, CDK6, CDK7, CDKN1C, CDKN2A, CDKN2B, CDKN2C, CDKN2D, CHEK1, CHEK2, Cyclin D1, E2F1, E2F2, E2F4, E2F5, EP300, GADD45A, GADD45B, GADD45G, GSK3B, HDAC1, HDAC2, MAD1L1, MAD2L1, MCM5, MCM7, MCMP2, MDM2, MYC, ORC3L, p21, p27/Kip1, p53, PCNA, PLK1, PRKDC, PTTG2, RB1, RBL1, RBL2, SKP2, Smad 4, SMAD2, SMAD3, SMC1A, STAG1, TFDP1, TFDP2, TGF beta 1, TGF beta 2, TGF beta 3, TTK, ZBTB17

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Antibody Array Glass Slide(s)
  • Spin Columns
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Streptavidin-Conjugated Fluor
  • Adhesive Plastic Strips
  • Labeling Buffer
  • Lysis Buffer
  • 30 ml Centrifuge Tube
Other Materials Required
  • Distilled or de-ionized water
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • 1 ml tube
  • Pipettors, pipette tips and other common lab consumables
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline
  1. Purify Samples with Spin Column
  2. Biotinylate Samples
  3. Purify biotinylated samples using spin column
  4. Dry the glass slide
  5. Block array surface
  6. Incubate with Samples
  7. Incubate with Streptavidin-Conjugated Fluor
  8. Disassemble the glass slide
  9. Scan with a gene microarray laser scanner
  10. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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# Manual

# SDS

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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