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Manual / SDS

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Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:

BAD | BCAR1 | CCL14 | CCL21 | CRKL | CXCL14 | FGR | FIP3 | FKHRL1 | GRK5 View more

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
B7-H4 Gene Symbol: VTCN1 B7H4 UNQ659/PRO1291 Gene ID: 79679 Accession #: Q7Z7D3 ELISA Kit: RayBio	Caspr2 Gene Symbol: CNTNAP2 CASPR2 KIAA0868 Gene ID: 26047 Accession #: Q9UHC6 ELISA Kit: RayBio Human Caspr2 ELISA	CD2 Gene Symbol: CD2 SRBC Gene ID: 914 Accession #: P06729 ELISA Kit: RayBio Human CD2 ELISA	CD4 Gene Symbol: CD4 Gene ID: 920 Accession #: P01730 ELISA Kit: RayBio Human CD4 ELISA	CD34 Gene Symbol: CD34 Gene ID: 947 Accession #: P28906 ELISA Kit: RayBio Human CD34 ELISA
CD51 (Integrin alpha-V) Gene Symbol: ITGAV MSK8 VNRA Gene ID: 3685 Accession #: P06756 ELISA Kit: RayBio Human CD51/Integrin alpha-V ELISA	CD86 (B7-2) Gene Symbol: CD86 CD28LG2 Gene ID: 942 Accession #: P42081 ELISA Kit: RayBio Human CD86/B7-2 ELISA	CD99-L2 Gene Symbol: CD99L2 MIC2L1 UNQ1964/PRO4486 Gene ID: 83692 Accession #: Q8TCZ2 ELISA Kit: RayBio Human CD99-L2 ELISA	IGSF4B (SynCAM3/CADM3) Gene Symbol: CADM3 IGSF4B NECL1 SYNCAM3 TSLL1 UNQ225/PRO258 Gene ID: 57863 Accession #: Q8N126 ELISA Kit: RayBio Human IGSF4B ELISA	Integrin alpha-4 (CD49d) Gene Symbol: CD49D ITGA4 Gene ID: 3676 Accession #: P13612 ELISA Kit: RayBio Human Integrin alpha-4/CD49d ELISA
Integrin alpha-M (CD11b/MAC-1) Gene Symbol: CD11B CR3A ITGAM Gene ID: 3684 Accession #: P11215 ELISA Kit: RayBio Human Integrin alpha-M/CD11b/MAC-1 ELISA	JAM-C (CD323) Gene Symbol: JAM3 UNQ859/PRO1868 Gene ID: 83700 Accession #: Q9BX67 ELISA Kit: RayBio Human JAM-C ELISA	LRRC4 Gene Symbol: LRRC4 BAG NAG14 UNQ554/PRO1111 Gene ID: 64101 Accession #: Q9HBW1 ELISA Kit: RayBio Human LRRC4 ELISA	N-Cadherin (Cadherin-2) Gene Symbol: CDH2 CDHN NCAD Gene ID: 1000 Accession #: P19022 ELISA Kit: RayBio	Nectin-2 (CD112) Gene Symbol: HVEB NECTIN2 PRR2 PVRL2 Gene ID: 5819 Accession #: Q92692 ELISA Kit: RayBio Human Nectin-2/CD112 ELISA
Nectin-3 Gene Symbol: PVRL3 PRR3 Gene ID: 25945 Accession #: Q9NQS3 ELISA Kit: RayBio Human Nectin-3 ELISA	Neurexin-3 beta (NRXN3) Gene Symbol: NRXN3 KIAA0743 Gene ID: 9369 Accession #: Q9HDB5 ELISA Kit: RayBio Human Neurexin-3 beta ELISA	Neuroligin 2 Gene Symbol: KIAA1366 NLGN2 Gene ID: 57555 Accession #: Q8NFZ4 ELISA Kit: RayBio Human Neuroligin 2 ELISA	P-Selectin Gene Symbol: SELP GMRP GRMP Gene ID: 6403 Accession #: P16109 ELISA Kit: RayBio Human P-Selectin ELISA	PD-L2 (B7-DC) Gene Symbol: PDCD1LG2 B7DC CD273 PDCD1L2 PDL2 Gene ID: 80380 Accession #: Q9BQ51 ELISA Kit: RayBio
Siglec-1 (CD169) Gene Symbol: SIGLEC1 SN Gene ID: 6614 Accession #: Q9BZZ2 ELISA Kit: RayBio Human Siglec-1 ELISA	Siglec-2 Gene Symbol: CD22 SIGLEC2 Gene ID: 933 Accession #: P20273 ELISA Kit: RayBio Human Siglec-2 ELISA	TIGIT Gene Symbol: TIGIT VSIG9 VSTM3 Gene ID: 201633 Accession #: Q495A1 ELISA Kit: RayBio Human TIGIT ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Chemokine Antibody Array C2 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Manual

SDS

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

CODE	Number of Membranes	Price
AAH-CHE-2-2	2 Sample Kit	$189.00
AAH-CHE-2-4	4 Sample Kit	$265.00
AAH-CHE-2-8	8 Sample Kit	$417.00