Human CRP ELISA

RayBio® Human CRP (C-Reactive Protein) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$1,680.00  
ELH-CRP-5
+

Assay Format

Detection Range:
34 pg/ml - 25,000 pg/ml
Recommended Dilution (serum & plasma):
20,000 fold
Sensitivity:
34 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GM-CSF, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P02741
Gene ID:
  • 1401
Gene Symbols:
  • CRP
  • PTX1
Protein Name & Synonyms:
C-reactive protein [Cleaved into: C-reactive protein(1-205)]
Target Species:
  • Human

Target Background

C-reactive protein (CRP) belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. CRP is synthesized by the liver in response to factors released by fat cells (adipocytes).Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. CRP is used mainly as a marker of inflammation. Apart from liver failure, there are few known factors that interfere with CRP production. Measuring and charting CRP values can prove useful in determining disease progress or the effectiveness of treatments. Blood, usually collected in a serum-separating tube, is analysed in a medical laboratory or at the point of care.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Verna E., Patel J., Bettencourt R., et al. Novel association between serum pentraxin-2 levels and advanced fibrosis in well-characterised patients with non-alcoholic fatty liver disease. Aliment Pharmacol Ther. 2015 Sep;42(5):582-90. doi: 10.1111/apt.13292.
    Species: Human
    Sample type: Serum (Advanced Liver Fibrosis in non-alcoholic fatty liner disease)
  2. Smeuninx, Benoit, et al. "Age-related anabolic resistance of myofibrillar protein synthesis is exacerbated in obese inactive individuals." The Journal of Clinical Endocrinology & Metabolism 102.9 (2017): 3535-3545.
    Species: Human
    Sample type: Plasma (hormone concentration)
  3. Vinayak, Shaveta, et al. "A clinical trial of lovastatin for modification of biomarkers associated with breast cancer risk." Breast cancer research and treatment 142.2 (2013): 389-398.
    Species: Human
    Sample type: Serum
  4. Zhang L., Wang X., Miao Y., et al. Magnetic ferroferric oxide nanoparticles induce vascular endothelial cell dysfunction and inflammation by disturbing autophagy. J Hazard Mater. 2016 Mar 5;304:186-95. doi: 10.1016/j.jhazmat.2015.10.041
    Species: Human
    Sample type: Conditioned Media (HUVEC cells treated with various ferroferric oxide nanoparticle comositions)
  5. Duan J, Yu Y, Yu Y, et al. Silica nanoparticles induce autophagy and endothelial dysfunction via the PI3K/Akt/mTOR signaling pathway. International Journal of Nanomedicine 2014;9:5131-5141. doi:10.2147/IJN.S71074.
    Species: Human
    Sample type: Conditioned Media (HUVEC conditioned media)
  6. Qian Y., Zhang S., Guo W., et al. Polychlorinated biphenyls (PCBs) inhibit hepcidin expression through an estrogen-like effect associated with disordered systemic iron homeostasis. Chem Res Toxicol. 2015 Apr 20;28(4):629-40. doi: 10.1021/tx500428r
    Species: Human
    Sample type: Conditioned Media (Human hepatocarcinoma cells, HepG2)
  7. Ciaffi L., Cavassini M., Genne D., et al. Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study. Eur J Clin Invest. 2015 Jul;45(7):720-30. doi: 10.1111/eci.12464.
    Species: Human
    Sample type: Serum (etravirine treated HIV patients)
  8. Rezvani R., et al. Cross sectional associations of Acylation Stimulating Protein (ASP) and adipose tissue gene expression with estradiol and progesterone in pre and postmenopausal women. Clinical Endocrinology. July 2014. DOI: 10.1111/cen.12540
    Species: Human
    Sample type: Plasma
  9. Negredo E, Jiménez M, Puig J, Loste C, Pérez-Álvarez N, Urrea V, Echeverría P, Bonjoch A, Clotet B, Blanco J. A randomized pilot trial to evaluate the benefit of the concomitant use of atorvastatin and Raltegravir on immunological markers in protease-inhibitor-treated subjects living with HIV. PLoS One. 2020 Sep 17;15(9):e0238575. doi: 10.1371/journal.pone.0238575. PMID: 32941476; PMCID: PMC7498036.
    Species: Human
    Sample type: Plasma
  10. Priya P., Rajappa M., Kattimani S., et al. Association of neurotrophins, inflammation and stress with suicide risk in young adults. Clin Chim Acta. 2016 Jun 1;457:41-5. doi: 10.1016/j.cca.2016.03.019.
    Species: Human
    Sample type: Serum (Suicide Risk of at risk patients based on inflammation, stress, and neurotrophins)

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

Recently Viewed Recently Viewed

Customers Also Bought Customers Also Bought