RayBio® Human CRP (C-Reactive Protein) ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • P02741
Gene ID:
  • 1401
Gene Symbols:
  • CRP
  • PTX1
Protein Name & Synonyms:
C-reactive protein [Cleaved into: C-reactive protein(1-205)]
Target Species:
  • Human

Assay Format

Detection Range:
34 pg/ml - 25,000 pg/ml
Recommended Dilution (serum & plasma):
20,000 fold
34 pg/ml

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This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GM-CSF, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Wang W., Song X., Chen Y., et al. The Long-Term Influence of Tissue Inhibitor of Matrix Metalloproteinase-1 in Patients with Mild to Moderate Coronary Artery Lesions in a Chinese Population: A 7-Year Follow-Up Study. Cardiology. 2015;132(3):151-8. doi: 10.1159/000430885
    Species: Human
    Sample type: Plasma
  2. Bil-lula I., et al. Infectobesity in the Polish Population - Evaluation of an Association between Adenoviruses Type 5, 31, 36 and Human Obesity. International Journal of Virology and Molecular Biology 2014, 3(1): 1-8. DOI: 10.5923/j.ijvmb.20140301.01
    Species: Human
    Sample type: Plasma
  3. Bil-Lula I., et al. Is There any Link Between Visceral Obesity and Adenovirus Infections in the Polish Population? Adv Clin Exp Med 2014, 23, 3, 415-422
    Species: Human
    Sample type: Plasma
  4. Duan J, Yu Y, Yu Y, et al. Silica nanoparticles induce autophagy and endothelial dysfunction via the PI3K/Akt/mTOR signaling pathway. International Journal of Nanomedicine 2014;9:5131-5141. doi:10.2147/IJN.S71074.
    Species: Human
    Sample type: Conditioned Media
  5. Rezvani R., et al. Cross sectional associations of Acylation Stimulating Protein (ASP) and adipose tissue gene expression with estradiol and progesterone in pre and postmenopausal women. Clinical Endocrinology. July 2014. DOI: 10.1111/cen.12540
    Species: Human
    Sample type: Plasma
  6. Wadood S., Shawk R., Baher H. Intercellular Adhesion Molecule-1, Vascular Adhesion Molecule-1, Interlukin1-?, and C - reactive protein Levels in Iraqi Patients with Type 2 Diabetes Mellitus. IOSR J of Dental and Medical SciencesVolume 13, Issue 4 Ver. IV. (Apr. 2014), PP55-62
    Species: Human
    Sample type: Serum
  7. Vinayak, Shaveta, et al. "A clinical trial of lovastatin for modification of biomarkers associated with breast cancer risk."?Breast cancer research and treatment?142.2 (2013): 389-398.
    Species: Human
    Sample type: Serum
  8. Arram, Eman O., Mohammed Saleh, and Enas Hammad. "Evaluation of B-type natriuretic peptide in patients with community acquired pneumonia."?Egyptian Journal of Chest Diseases and Tuberculosis?62.2 (2013): 293-300.
    Species: Human
    Sample type: Serum
  9. Zhang J., Du P., Gao J., et al. Preoperative probiotics decrease postoperative infectious complications of colorectal cancer. Am J Med Sci. 2012 Mar;343(3):199-205. doi: 10.1097/MAJ.0b013e31823aace6.
    Species: Human
    Sample type: Serum

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