Human Cytokine Array C1

RayBio® C-Series Human Cytokine Antibody Array 1 Kit. Detects 23 Humans Cytokines. Suitable for all liquid sample types.

$944.35  
AAH-CYT-1-8
+

Antigen Information

Gene Symbols:
CCL2 | CCL5 | CCL7 | CCL8 | CSF2 | CSF3 | CXCL1 | CXCL8 | CXCL9 | IFNG   View more

Assay Format

Number of Targets Detected:
23
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

GCSF
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
IL-1 alpha
(IL-1 F1)
IL-2
IL-3
IL-5
IL-6
IL-7
IL-8
(CXCL8)
IL-10
IL-13
IL-15
IFN-gamma
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MIG
(CXCL9)
RANTES
(CCL5)
TGF beta 1
TNF alpha
TNF beta
(TNFSF1B)
   

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Dou Z., Ghosh K., Vizioli M., et al. Cytoplasmic chromatin triggers inflammation in senescence and cancer. Nature. 2017 October 19; 550(7676): 402–406. doi:10.1038/nature24050.
    Species: Human
    Sample type: Conditioned Media (IMR90 senescent cell model)
  2. Yang H., Wang H., Ju Z., et al. MD-2 is required for disulfide HMGB1-dependent TLR4 signaling. J Exp Med. 2015 Jan 12;212(1):5-14. doi: 10.1084/jem.20141318.
    Species: Human
    Sample type: Conditioned Media (Human Primary Macropahges stimulated with LPS)
  3. Ma C, Witkowski MT, Harris J, Dolgalev I, Sreeram S, Qian W, Tong J, Chen X, Aifantis I, Chen W. Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche. Sci Adv. 2020 Oct 30;6(44):eaba5536. doi: 10.1126/sciadv.aba5536. PMID: 33127669; PMCID: PMC7608809.
    Species: Human
    Sample type: Conditioned Media
  4. Zhao, B., Liu, P., Fukumoto, T. et al. Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence. Nat Commun 11, 908 (2020). https://doi.org/10.1038/s41467-020-14652-y
    Species: Human
    Sample type: Conditioned media (Cell culture media)
  5. Sohara Y, Shimada H, Minkin C, Erdreich-Epstein A, Nolta J,et al. Bone Marrow Mesenchymal Stem Cells Provide an Alternate Pathway of Osteoclast Activation and Bone Destruction by Cancer Cells. Cancer Res. 2005;65(4):1129-1135
    Species: Human
    Sample type: Conditioned Media (coclutures of cells)
  6. Fujiwara, H et al. In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins. In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins. In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins. Clin. Cancer Res. Volume 11, Issue 12, Pages 4495-503, Jun 2005
    Species: Human
    Sample type: Conditioned Media (CD40-B cells cultured with LTK-CD40L cells supernatant)
  7. Liu, Yan, et al. "Targeting aurora kinases limits tumour growth through DNA damage?mediated senescence and blockade of NF??B impairs this drug?induced senescence." EMBO molecular medicine 5.1 (2013): 149-166.
    Species: Human
    Sample type: Conditioned Media (Hs294T cells - MLN8237-treated melanoma cells )
  8. Sos M., et al. Oncogene Mimicry as a Mechanism of Primary Resistance to BRAF Inhibitors. Cell Reports Available online 7 August 2014. DOI: 10.1016/j.celrep.2014.07.010
    Species: Human
    Sample type: Conditioned Media (BRAF resistant cancer cell lines)
  9. Desai, Mauli B., et al. "Pollen?induced antigen presentation by mesenchymal stem cells and T cells from allergic rhinitis." Clinical & translational immunology 2.10 (2013).
    Species: Human
    Sample type: Conditioned Media (PBMCs, with pulsed and unpulsed MSCs)
  10. Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007; 6: 1680-1693
    Species: Human
    Sample type: Conditioned Media (conditioned or non-conditioned medium)

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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