Human Cytokine Array C1

RayBio® C-Series Human Cytokine Antibody Array 1 Kit. Detects 23 Humans Cytokines. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
CCL2 | CCL5 | CCL7 | CCL8 | CSF2 | CSF3 | CXCL1 | CXCL8 | CXCL9 | IFNG   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

GRO alpha/beta/gamma
GRO alpha
IL-1 alpha
(IL-1 F1)
TGF beta 1
TNF alpha
TNF beta

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Zhao, B., Liu, P., Fukumoto, T. et al. Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence. Nat Commun 11, 908 (2020).
    Species: Human
    Sample type: Conditioned media
  2. Ma C, Witkowski MT, Harris J, Dolgalev I, Sreeram S, Qian W, Tong J, Chen X, Aifantis I, Chen W. Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche. Sci Adv. 2020 Oct 30;6(44):eaba5536. doi: 10.1126/sciadv.aba5536. PMID: 33127669; PMCID: PMC7608809.
    Species: Human
    Sample type: Conditioned Media
  3. Dou Z., Ghosh K., Vizioli M., et al. Cytoplasmic chromatin triggers inflammation in senescence and cancer. Nature. 2017 October 19; 550(7676): 402–406. doi:10.1038/nature24050.
    Species: Human
    Sample type: Conditioned Media
  4. Haderk, Franziska, et al. "Tumor-derived exosomes modulate PD-L1 expression in monocytes." Science immunology 2.13 (2017): eaah5509.
    Species: human
    Sample type: Serum
  5. Arabzadeh S., Hossein G., Zarnani A. Wnt5A exerts immunomodulatory activity in the human ovarian cancer cell line SKOV-3. Cell Biol Int. 2016 Feb;40(2):177-87. doi: 10.1002/cbin.10551
    Species: Human
    Sample type: Conditioned Media
  6. Ehnert S., Treude T., Ihle C., et al. Factors circulating in the blood of type 2 diabetes mellitus patients affect osteoblast maturation - description of a novel in vitro model. Exp Cell Res. 2015 Mar 15;332(2):247-58. doi: 10.1016/j.yexcr.2014.12.011
    Species: Human
    Sample type: Serum
  7. Fierro N., Gonzalez-Aldavo K., Torres-Valadez R., et al. Spontaneous hepatitis C viral clearance and hepatitis C chronic infection are associated with distinct cytokine profiles in Mexican patients. Mem Inst Oswaldo Cruz. 2015 Apr; 110(2): 267-271. doi: 10.1590/0074-02760140377
    Species: Human
    Sample type: Serum
  8. Park M., Kim YH., Ryu JH., et al. Immune Suppressive Effects of Tonsil-Derived Mesenchymal Stem Cells on Mouse Bone-Marrow-Derived Dendritic Cells. Stem Cells Int. 2015;2015:106540. doi: 10.1155/2015/106540.
    Species: Human
    Sample type: Conditioned Media
  9. Yang H., Wang H., Ju Z., et al. MD-2 is required for disulfide HMGB1-dependent TLR4 signaling. J Exp Med. 2015 Jan 12;212(1):5-14. doi: 10.1084/jem.20141318.
    Species: Human
    Sample type: Conditioned Media
  10. Stone A., Vanderman K., Willey J., et al. Osteoarthritic changes in vervet monkey knees correlate with meniscus degradation and increased matrix metalloproteinase and cytokine secretion. Osteoarthritis Cartilage. 2015 Oct;23(10):1780-9. doi: 10.1016/j.joca.2015.05.020.
    Species: Non-Human Primate
    Sample type: Conditioned Media

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