Human Cytokine Array C12

RayBio® C-Series Human Cytokine Antibody Array 12 Kit. Detects 32 Human Cytokines. Suitable for all liquid sample types.

$440.36  
AAH-CYT-12-2
+

Antigen Information

Gene Symbols:
AHSG | ALB | AMICA1 | ANGPT4 | CD163 | CLU | CRTAM | CSF3R | CST3 | CXCL14   View more

Assay Format

Number of Targets Detected:
32
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
aFGF
(FGF-1)
Albumin
AMICA
Angiopoietin-4
BAFF
  • Gene Symbol: TNFSF13B
  • Gene ID: 10673
  • Accession #: Q9Y275
CD163
Clusterin
CRTAM
CXCL14
(BRAK)
Cystatin C
DLL1
Fetuin A
FOLR1
GASP-1
(WFIKKNRP)
GASP-2
(WFIKKN)
GCSF R
(CD114)
HAI-2
IL-17 RB
IL-27
  • Gene Symbol: IL27
  • Gene ID: 246778
  • Accession #: Q8NEV9
LAG-3
LDL R
Pepsinogen 1
RBP4
SOST
Syndecan-1
TACI
(TNFRSF13B)
TFPI
Thrombospondin 1
TRANCE
(TNFSF11)
  • Gene Symbol: TNFSF11
  • Gene ID: 8600
  • Accession #: O14788
Troponin I
uPA
WISP-1
(CCN4)

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C12 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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