Human Cytokine Array C13

RayBio® C-Series Human Cytokine Array 13 Kit. Detects 40 human cytokines. Suitable for all liquid sample types. 4-8 week lead time.

$427.23  
AAH-CYT-13-2
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Assay Format

Number of Targets Detected:
40
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
ACE | ACVR1B | ADAM23 | ARTN | CTF1 | CTSV | FABP1 | FGF20 | GDF6 | GRN   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ACE
(CD143)
Activin R1B
(ALK-4)
ADAM23
Artemin
Cardiotrophin-1
(CT-1)
Cathepsin V
(Cathepsin L2)
FABP1
  • Gene Symbol: FABP1 FABPL
  • Gene ID: 2168
  • Accession #: P07148
FGF-20
GDF-8
(Myostatin)
HAI-1
IL-27 R alpha
Insulin R
(CD220)
  • Gene Symbol: INSR
  • Gene ID: 3643
  • Accession #: P06213
Kallikrein 7
LIF R alpha
Lipocalin-1
Lymphotoxin beta R
(TNF RIII)
Mesothelin
MFRP
Neuropilin-2
Neurturin
Nidogen-2
Olfactomedin-2
(Noelin-2)
p53
PD-ECGF
(Thymidine Phosphorylase)
PDGF-C
Progranulin
Ret
ROBO4
Semaphorin 6B
SERPINF1
(PEDF)
SREC-I
(SCARF1)
SREC-II
(SCARF2)
TLR1
TLR3
TREM-2
Tripeptidyl-peptidase I
(TPP1)
TRKC
TROY
(TNFRSF19)
Uromodulin
XIAP

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C13 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.