Human Cytokine Array C15

RayBio® C-Series Human Cytokine Array 15 Kit. Detects 40 human cytokines. Suitable for all liquid sample types. 4-8 week lead time.

$427.23  
AAH-CYT-15-2
+

Assay Format

Number of Targets Detected:
40
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
AMIGO1 | AMN | ANTXR2 | ARSA | BCL2L2 | BST1 | CD109 | CD207 | CD34 | CD83   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ADP-Ribosyl Cyclase 2
(BST-1/CD157)
AmiGO
Aminopeptidase LRAP
(ERAP2)
Amnionless
Arylsulfatase A
(ARSA)
bcl-w
CD109
CD34
CD83
CLEC-1
CLEC10A
(CD301)
CMG-2
(ANTXR2)
CREG
Cystatin SN
Cytokeratin 8
(KRT8)
Dectin-1
(CLEC7A)
Desmocollin-3
Endoglycan
(PODXL2)
Galectin-4
HAPLN1
Jagged 1
Langerin
(CD207)
Lumican
Matriptase
(ST14)
Meprin beta
(MEP1B)
Nectin-3
OX40
(TNFRSF4)
OX40 Ligand
(TNFSF4)
p27/Kip1
(CDKN1B)
PAPP-A2
Plexin B3
Plexin D1
ProGRP
  • Gene Symbol: GRP
  • Gene ID: 2922
  • Accession #: P07492
PSA-total
Reg1B
RGM-A
ROBO2
Spinesin
TWEAK R
(TNFRSF12)
ULBP-3

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C15 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.