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770-729-2992
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| CODE | Number of Membranes | Price | ||
|---|---|---|---|---|
|
AAH-CYT-15-2
|
$427.00 | ||
|
AAH-CYT-15-4
|
$598.00 | ||
|
AAH-CYT-15-8
|
$940.00 |
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Assay Format
Number of Targets Detected:
40
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Membrane
Product Specifications
Size:
2, 4, or 8 Sample Kit
Protein Information
Gene Symbols:
AMIGO1 | AMN | ANTXR2 | ARSA | BCL2L2 | BST1 | CD109 | CD207 | CD34 | CD83| CDKN1B | CLEC10A | CLEC1A | CLEC7A | CREG1 | CST1 | DSC3 | ERAP2 | GRP | HAPLN1 | JAG1 | KLK3 | KRT8 | LGALS4 | LUM | MEP1B | PAPPA2 | PLXNB3 | PLXND1 | PODXL2 | PVRL3 | REG1B | RGMA | ROBO2 | ST14 | TMPRSS5 | TNFRSF4 | TNFSF12 | TNFSF4 | ULBP3 View more
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
ADP-Ribosyl Cyclase 2 (BST-1/CD157)
| AmiGO
| Aminopeptidase LRAP (ERAP2)
| Amnionless
| Arylsulfatase A (ARSA)
|
bcl-w
| CD109
| CD34
| CD83
| CLEC-1
|
CLEC10A (CD301)
| CMG-2 (ANTXR2)
| CREG
| Cystatin SN
| Cytokeratin 8 (KRT8)
|
Dectin-1 (CLEC7A)
| Desmocollin-3
| Endoglycan (PODXL2)
| Galectin-4
| HAPLN1
|
Jagged 1
| Langerin (CD207)
| Lumican
| Matriptase (ST14)
| Meprin beta (MEP1B)
|
Nectin-3
| OX40 (TNFRSF4)
| OX40 Ligand (TNFSF4)
| p27/Kip1 (CDKN1B)
| PAPP-A2
|
Plexin B3
| Plexin D1
| ProGRP
| PSA-total
| Reg1B
|
RGM-A
| ROBO2
| Spinesin
| TWEAK R (TNFRSF12)
| ULBP-3
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C15 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
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