Human Cytokine Array C16

RayBio® C-Series Human Cytokine Array 16 Kit. Detects 40 human cytokines. Suitable for all liquid sample types. 4-8 week lead time.

$427.23  
AAH-CYT-16-2
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Assay Format

Number of Targets Detected:
40
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
ACVR2A | BGN | CA13 | CA2 | CA72-4 | CLEC1B | CLEC4G | CST5 | EPO | FCRL5   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin R2A
Biglycan
CA13
CA2
CLEC-2
c-Myc
(MYC)
Cystatin D
Erythropoietin
FCRL5
(FcRH5)
FGF-16
GATA4
GFR alpha-1
(GDNF R)
GFR alpha-2
Granzyme B
Granzyme H
HIF-1 alpha
PAPP-A Heterotetramic
(htPAPP-A)
IFN-beta
  • Gene Symbol: IFNB1 IFB IFNB
  • Gene ID: 3456
  • Accession #: P01574
IL-17 RC
IL-19
IL-20 R beta
IL-22
ILT-4
(LIR-2/CD85d)
LAIR2
LSECtin
(CLEC4G)
Netrin-4
Norrin
NRG1-alpha
(HRG1-alpha)
PD-L2
(B7-DC)
  • Gene Symbol: PDCD1LG2 B7DC CD273 PDCD1L2 PDL2
  • Gene ID: 80380
  • Accession #: Q9BQ51
PDX-1
(IPF1)
Podocalyxin
RGM-C
(Hemojuvelin)
S100 A1
Semaphorin 6A
SLITRK5
SR-AI
(MSR)
ST6GAL1
Thyroid Peroxidase
Troponin C

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C16 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.