Human Cytokine Array C17

RayBio® C-Series Human Cytokine Array 17 Kit. Detects 40 human cytokines. Suitable for all liquid sample types. 4-8 week lead time.

$427.23  
AAH-CYT-17-2
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Assay Format

Number of Targets Detected:
40
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
ACVR1 | B4GALT1 | BAI1 | C1QTNF4 | CA14 | CA4 | CA6 | CA8 | CD300A | CD300E   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin R1A
(ALK-2)
ASAHL
B4GalT1
BAI1
Brorin
(VWC2)
C1qTNF4
CA14
CA4
CA6
CA8
Cadherin-6
(KCAD)
Caspr2
CD27 Ligand
(TNFSF7)
CD300a
(LMIR1)
CD300e
(LMIR6)
CD300f
(CLM-1/IREM-1)
CD4
CD5
CD69
Cytokeratin 18
(KRT18)
Cytokeratin 19
(KRT19)
Carboxypeptidase B1
(CPB1)
CRISP-2
DDR1
Fucosyltransferase 8
(FUT8)
MIA
NTAL
NTB-A
(SLAMF6)
Omgp
PEAR1
Podoplanin
PTH1R
Reg4
ROR1
Semaphorin 4G
SERPINA5
(Protein C Inhibitor)
Serpin B6
Siglec-1
(CD169)
Sirtuin 2
Sirtuin 5

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C17 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.