Human Cytokine Array C6

RayBio® C-Series Human Cytokine Antibody Array 6 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

$616.50  
AAH-CYT-6-4
+

Antigen Information

Gene Symbols:
ANG | BDNF | BMP4 | BMP6 | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18   View more

Assay Format

Number of Targets Detected:
60
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
BDNF
BLC
(CXCL13)
BMP-4
BMP-6
Ck beta 8-1
(CCL23)
CNTF
EGF
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
FGF-6
FGF-7
(KGF)
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GDNF
GM-CSF
I-309
(TCA-3/CCL1)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-4
IGF-1
IL-10
IL-13
IL-15
IL-16
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
Leptin
Light
(TNFSF14)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
NAP-2
(PPBP/CXCL7)
NT-3
PARC
(CCL18)
PDGF-BB
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TNF alpha
TNF beta
(TNFSF1B)

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C6 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Maury E, Ehala-Aleksejev K, Guiot Y, Detry R, Vandenhooft A, Brichard SM. Adipokines oversecreted by omental adipose tissue in human obesity. Am J Physiol Endocrinol Metab. 2007; 293:E656-E665.
    Species: Human
    Sample type: Conditioned Media
  2. Meltzer K, Standley P.Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion.J Am Osteopath Assoc. 2007;107:527-536
    Species: Human
    Sample type: Conditioned Media
  3. Meltzer K., Standley P., Modeled Repetitive Motion Strain and Indirect Osteopathic Manipulative Techniques in Regulation of Human Fibroblast Proliferation and Interleukin Secretion. J Am Osteopath Assoc December 1, 2007 vol. 107 no. 12 527-536
    Species: Human
    Sample type: Conditioned Media
  4. Moro C, Klimeakova E, Lolmede K, Berlan M, et al. Atrial natriuretic peptide inhibits the production of adipokines and cytokines linked to inflammation and insulin resistance in human subcutaneous adipose tissue. Diabetologia. 2007; 50:1038-1047.
    Species: Human
    Sample type: Conditioned Media
  5. Parekkadan B, van Poll D, Suganuma K, Carter EA, Berthiaume F, et al. Mesenchymal Stem Cell-Derived Molecules Reverse Fulminant Hepatic Failure. PLoS ONE.2007;2(9): e941. doi:10.1371/journal.pone.0000941
    Species: Human
    Sample type: Conditioned Media
  6. Ray S, Britschgi M, Herbert C, Takeda-Uchimura Y, et al. Classification and prediction of clinical Alzheimer's diagnosis based on plasma signaling proteins. Nat Med. 2007; 13(11):1359-1362.
    Species: Human
    Sample type: Plasma
  7. Wang CC, Yim KW, Poon TCW, Choi WK, et al. Innate Response by Ficolin Binding in Apoptotic Placenta is Associated with the Clinical Syndrome of Preeclampsia. Clin Chem. 2007;53:42-52.
    Species: Human
    Sample type: Tissue Lysate
  8. Wang Y, Cai D, Brendel C, Barett C, Erben P, et al. Adaptive secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates imatinib and nilotinib resistance in BCR/ABL+ progenitors via JAK-1/STAT-5 pathway activation. Blood. 2007;109:2147-2155.
    Species: Human
    Sample type: Conditioned Media
  9. Ancuta P, Wang J, Gabuzda D. CD16+ monocytes produce IL-6, CCL2, and matrix metalloproteinase-9 upon interacting with CX3CL1-expressing endothelial cells. J Leuk Biol. 2006;80:1156-1164.
    Species: Human
    Sample type: Conditioned Media
  10. Celis JE, Gromov P, Moreira JMA, Cabezon T, et al. Apocrine Cysts of the Breast: Biomarkers, Origin, Enlargement, and Relation with Cancer Phenotype. Mol Cell Proteomics. 2006;5:432-483.
    Species: Human
    Sample type: Cyst Fluid, Breast

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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