Human Cytokine Array C7

RayBio® C-Series Human Cytokine Antibody Array 7 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

$616.50  
AAH-CYT-7-4
+

Antigen Information

Gene Symbols:
ADIPOQ | AGRP | ANGPT2 | AREG | AXL | BTC | CCL16 | CCL19 | CCL25 | CCL27   View more

Assay Format

Number of Targets Detected:
60
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
AgRP
Angiopoietin-2
Amphiregulin
Axl
bFGF
beta-NGF
Betacellulin
(BTC)
CCL28
(MEC)
CTACK
(CCL27)
Dtk
EGFR
ENA-78
(CXCL5)
Fas
(TNFRSF6/Apo-1)
FGF-4
FGF-9
GCSF
GITR Ligand
(TNFSF18)
GITR
(TNFRSF18)
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
ICAM-1
(CD54)
ICAM-3
(CD50)
IGFBP-3
IGFBP-6
IGF-1 R
IL-1 R4
(ST2)
IL-1 R1
IL-11
IL-12 p40
IL-12 p70
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-2 R alpha
IL-6 R
IL-8
(CXCL8)
I-TAC
(CXCL11)
Lymphotactin
(XCL1)
MIF
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-3 beta
(CCL19)
MSP alpha/beta
NT-4
Osteoprotegerin
(TNFRSF11B)
Oncostatin M
PLGF
gp130
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TECK
(CCL25)
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VEGF-A
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C7 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Ancuta P, Wang J, Gabuzda D. CD16+ monocytes produce IL-6, CCL2, and matrix metalloproteinase-9 upon interacting with CX3CL1-expressing endothelial cells. J Leuk Biol. 2006;80:1156-1164.
    Species: Human
    Sample type: Conditioned Media
  2. Celis JE, Gromov P, Moreira JMA, Cabezon T, et al. Apocrine Cysts of the Breast: Biomarkers, Origin, Enlargement, and Relation with Cancer Phenotype. Mol Cell Proteomics. 2006;5:432-483.
    Species: Human
    Sample type: Cyst Fluid, Breast
  3. Falkensammer C, Johrer K, Gander H, Ramoner R, Putz T, et al.IL-4 inhibits the TNF-a induced proliferation of renal cell carcinoma (RCC) and cooperates with TNF-a to induce apoptotic and cytokine responses by RCC: implications for antitumor immune responses. Cancer Immunol Immunother.2006; 55: 1228-1237 DOI 10.1007/s00262-006-0122-1
    Species: Human
    Sample type: Conditioned Media
  4. Liu B-C, Zhang L, Lv L, Wang Y, Liu D, Zhang X. Application of Antibody Array Technology in the Analysis of Urinary Cytokine Profiles in Patients with Chronic Kidney Disease. Am J Nephrol. 2006;26(5):483-490.
    Species: Human
    Sample type: Urine
  5. Celis JE, Moreira JMA, CabezÃ?n T, Gromov P, et al. Identification of Extracellular and Intracellular Signaling Components of the Mammary Adipose Tissue and Its Interstitial Fluid in High Risk Breast Cancer Patients: Toward Dissecting The Molecular Circuitry of Epithelial-Adipocyte Stromal Cell Interactions. Mol Cell Proteomics. 2005;4:492-522.
    Species: Human
    Sample type: Interstitial Fluid, Breast
  6. Celis JE, Moreira JMA, Gromova I, Cabezon T, et al. Towards discovery-driven translational research in breast cancer. FEBS J. 2005;272:
    Species: Human
    Sample type: Interstitial Fluid, Breast
  7. Dietze-Schroeder D, Sell H, Uhlig M, Koenen M, Eckel J. Autocrine Action of Adiponectin on Human Fat Cells Prevents the Release of Insulin Resistance-Inducing Factors. Diabetes. 2005;54:2003-2011.
    Species: Human
    Sample type: Conditioned Media
  8. Giroux M, Denis F. Human CD1d-unrestricted NKT cells release chemokines upon Fas engagement. Blood. 2005;105(2):703-710.
    Species: Human
    Sample type: Conditioned Media
  9. Henrichot E, Juge-Aubry CE, Pernin A, Pache J-C, et al. Production of Chemokines by Perivascular Adipose Tissue: A Role in the Pathogenesis of Atherosclerosis? Aterioscler Thromb Vasc Biol. 2005;25:2594-2599
    Species: Human
    Sample type: Conditioned Media
  10. Celis JE, Moreira JMA, Cabezón T, Gromov P, et al. Identification of Extracellular and Intracellular Signaling Components of the Mammary Adipose Tissue and Its Interstitial Fluid in High Risk Breast Cancer Patients: Toward Dissecting The Molecular Circuitry of Epithelial-Adipocyte Stromal Cell Interactions. Mol Cell Proteomics. 2005;4:492-522.
    Species: Human
    Sample type: Interstitial Fluid, Breast

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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