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Home/Search results/Products/Arrays/Antibody Arrays/C-Series/Cytokine Arrays/Human Cytokine Array C8

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Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:

ALCAM | BMP5 | BMP7 | CCL23 | CD14 | CD80 | CDH5 | CSF1R | CTF1 | CXCL10 View more

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin A Gene Symbol: INHBA Gene ID: 3624 Accession #: P08476 ELISA Kit: RayBio Human Activin A ELISA	ALCAM (CD166) Gene Symbol: ALCAM Gene ID: 214 Accession #: Q13740 ELISA Kit: RayBio Human ALCAM ELISA	CD80 (B7-1) Gene Symbol: CD80 Gene ID: 941 Accession #: P33681 ELISA Kit: RayBio Human CD80 ELISA	BMP-5 Gene Symbol: BMP5 Gene ID: 653 Accession #: P22003 ELISA Kit: RayBio Human BMP-5 ELISA	BMP-7 Gene Symbol: BMP7 Gene ID: 655 Accession #: P18075 ELISA Kit: RayBio Human BMP-7 ELISA
Cardiotrophin-1 (CT-1) Gene Symbol: CTF1 Gene ID: 1489 Accession #: Q16619 ELISA Kit: RayBio Human CT-1 ELISA	CD14 Gene Symbol: CD14 Gene ID: 929 Accession #: P08571 ELISA Kit: RayBio Human CD14 ELISA	CXCL16 Gene Symbol: CXCL16 Gene ID: 58191 Accession #: Q9H2A7 ELISA Kit: RayBio Human CXCL16 ELISA	DR6 (TNFRSF21) Gene Symbol: TNFRSF21 Gene ID: 27242 Accession #: O75509 ELISA Kit: RayBio Human DR6 ELISA	Endoglin (CD105) Gene Symbol: ENG Gene ID: 2022 Accession #: P17813 ELISA Kit: RayBio Human Endoglin ELISA
ErbB3 Gene Symbol: ErbB3 Gene ID: 2065 Accession #: P21860 ELISA Kit: RayBio Human ErbB3 ELISA	E-Selectin Gene Symbol: SELE Gene ID: 6401 Accession #: P16581 ELISA Kit: RayBio Human E-Selectin ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG Gene ID: 356 Accession #: P48023 ELISA Kit: RayBio Human FasL ELISA	ICAM-2 (CD102) Gene Symbol: ICAM2 Gene ID: 3384 Accession #: P13598 ELISA Kit: RayBio Human ICAM-2 ELISA	IGF-2 Gene Symbol: Igf2 Gene ID: 3481 Accession #: P01344
IL-1 R2 Gene Symbol: IL1R2 Gene ID: 7850 Accession #: P27930 ELISA Kit: RayBio Human IL-1 sRII ELISA	IL-10 R beta Gene Symbol: IL10RB Gene ID: 3588 Accession #: Q08334 ELISA Kit: RayBio Human IL-10 R beta ELISA	IL-13 R alpha 2 Gene Symbol: IL13RA2 Gene ID: 3598 Accession #: Q14627 ELISA Kit: RayBio Human IL-13 Ra2 ELISA	IL-18 BP alpha Gene Symbol: IL18BP Gene ID: 10068 Accession #: O95998 ELISA Kit: RayBio Human IL-18 BPa ELISA	IL-18 R beta (AcPL) Gene Symbol: IL18RAP Gene ID: 8807 Accession #: O95256 ELISA Kit: RayBio Human IL-18 R beta ELISA
MMP-3 Gene Symbol: MMP3 Gene ID: 4314 Accession #: P08254 ELISA Kit: RayBio Human MMP-3 ELISA	IL-2 R beta (CD122) Gene Symbol: IL2RB Gene ID: 3560 Accession #: P14784 ELISA Kit: RayBio Human IL-2 R beta ELISA	IL-2 R gamma (Common gamma Chain) Gene Symbol: IL2RG Gene ID: 3561 Accession #: P31785	IL-21 R Gene Symbol: IL21R Gene ID: 50615 Accession #: Q9HBE5 ELISA Kit: RayBio Human IL-21 R ELISA	IL-5 R alpha Gene Symbol: IL5RA Gene ID: 3568 Accession #: Q01344 ELISA Kit: RayBio Human IL-5 R alpha ELISA
IL-9 Gene Symbol: IL9 Gene ID: 3578 Accession #: P15248 ELISA Kit: RayBio Human IL-9 ELISA	IP-10 (CXCL10) Gene Symbol: CXCL10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA	LAP/TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137	Leptin R Gene Symbol: LEPR Gene ID: 3953 Accession #: P48357	LIF Gene Symbol: LIF Gene ID: 3976 Accession #: P15018 ELISA Kit: RayBio Human LIF ELISA
L-Selectin (CD62L) Gene Symbol: SELL Gene ID: 6402 Accession #: P14151 ELISA Kit: RayBio Human L-Selectin ELISA	M-CSF R Gene Symbol: CSF1R Gene ID: 1436 Accession #: P07333 ELISA Kit: RayBio Human M-CSF R ELISA	MMP-1 Gene Symbol: MMP1 Gene ID: 4312 Accession #: P03956 ELISA Kit: RayBio Human MMP-1 ELISA	MMP-13 Gene Symbol: MMP13 Gene ID: 4322 Accession #: P45452 ELISA Kit: RayBio Human MMP-13 ELISA	MMP-9 Gene Symbol: MMP9 Gene ID: 4318 Accession #: P14780 ELISA Kit: RayBio Human MMP-9 ELISA
MPIF-1 (CCL23) Gene Symbol: CCL23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human MPIF-1 ELISA	NGFR (TNFRSF16) Gene Symbol: NGFR Gene ID: 4804 Accession #: P08138 ELISA Kit: RayBio Human NGF R ELISA	PDGF-AA Gene Symbol: PDGFA Gene ID: 5154 Accession #: P04085 ELISA Kit: RayBio Human PDGF-AA ELISA	PDGF-AB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-AB ELISA	PDGF R alpha Gene Symbol: PDGFRA Gene ID: 5156 Accession #: P16234 ELISA Kit: RayBio Human PDGF R alpha ELISA
PDGF R beta Gene Symbol: PDGFRB Gene ID: 5159 Accession #: P09619 ELISA Kit: RayBio Human PDGF R beta ELISA	PECAM-1 (CD31) Gene Symbol: PECAM1 Gene ID: 5175 Accession #: P16284 ELISA Kit: RayBio Human PECAM-1 ELISA	Prolactin Gene Symbol: PRL Gene ID: 5617 Accession #: P01236	SCF R (CD117/c-kit) Gene Symbol: KIT Gene ID: 3815 Accession #: P10721 ELISA Kit: RayBio Human SCF R ELISA	SDF-1 beta (CXCL12 beta) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 beta ELISA
Siglec-5 (CD170) Gene Symbol: SIGLEC5 Gene ID: 8778 Accession #: O15389 ELISA Kit: RayBio Human Siglec-5 ELISA	TGF alpha Gene Symbol: TGFA Gene ID: 7039 Accession #: P01135 ELISA Kit: RayBio Human TGF-alpha ELISA	TGF beta 2 Gene Symbol: TGFB2 Gene ID: 7042 Accession #: P61812 ELISA Kit: RayBio Human TGF-beta 2 ELISA	Tie-1 Gene Symbol: TIE1 Gene ID: 7075 Accession #: P35590 ELISA Kit: RayBio Human Tie-1 ELISA	Tie-2 Gene Symbol: TEK Gene ID: 7010 Accession #: Q02763 ELISA Kit: RayBio Human Tie-2 ELISA
TIMP-4 Gene Symbol: TIMP4 Gene ID: 7079 Accession #: Q99727 ELISA Kit: RayBio Human TIMP-4 ELISA	VE-Cadherin (CDH5) Gene Symbol: CDH5 Gene ID: 1003 Accession #: P33151	VEGFR2 Gene Symbol: KDR Gene ID: 3791 Accession #: P35968 ELISA Kit: RayBio Human VEGF R2 ELISA	VEGFR3 Gene Symbol: FLT4 Gene ID: 2324 Accession #: P35916 ELISA Kit: RayBio Human VEGF R3 ELISA

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array C8 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.