Human Cytokine Array C8

RayBio® C-Series Human Cytokine Antibody Array 8 Kit. Detects 54 Human Cytokines. Suitable for all liquid sample types.

$616.50  
AAH-CYT-8-4
+

Assay Format

Number of Targets Detected:
54
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
ALCAM | BMP5 | BMP7 | CCL23 | CD14 | CD80 | CDH5 | CSF1R | CTF1 | CXCL10   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Activin A
ALCAM
(CD166)
CD80
(B7-1)
BMP-5
BMP-7
Cardiotrophin-1
(CT-1)
CD14
CXCL16
DR6
(TNFRSF21)
Endoglin
(CD105)
ErbB3
E-Selectin
Fas Ligand
(TNFSF6)
ICAM-2
(CD102)
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IL-1 R2
IL-10 R beta
IL-13 R alpha 2
IL-18 BP alpha
IL-18 R beta
(AcPL)
MMP-3
IL-2 R beta
(CD122)
IL-2 R gamma
(Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-21 R
IL-5 R alpha
IL-9
IP-10
(CXCL10)
LAP/TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: 3953
  • Accession #: P48357
LIF
L-Selectin
(CD62L)
M-CSF R
MMP-1
MMP-13
MMP-9
MPIF-1
(CCL23)
NGFR
(TNFRSF16)
PDGF-AA
PDGF-AB
PDGF R alpha
PDGF R beta
PECAM-1
(CD31)
Prolactin
  • Gene Symbol: PRL
  • Gene ID: 5617
  • Accession #: P01236
SCF R
(CD117/c-kit)
SDF-1 beta
(CXCL12 beta)
Siglec-5
(CD170)
TGF alpha
TGF beta 2
Tie-1
Tie-2
TIMP-4
VE-Cadherin
(CDH5)
  • Gene Symbol: CDH5
  • Gene ID: 1003
  • Accession #: P33151
VEGFR2
VEGFR3

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C8 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Barr P., Li H., Spier C., et al. Phase II Intergroup Trial of Alisertib in Relapsed and Refractory Peripheral T-Cell Lymphoma and Transformed Mycosis Fungoides: SWOG 1108. J Clin Oncol. 2015 Jul 20;33(21):2399-404. doi: 10.1200/JCO.2014.60.6327.
    Species: Human
    Sample type: Serum (Alisertib treated T-cell lyphoma treatment)
  2. Chen W., Ho C., Chang Y., Chen H., Lin C., et al. Cancer-associated fibroblasts regulate the plasticity of lung cancer stemness via paracrine signalling. Nat. Comm. 2014, 5, article 3472. doi:10.1038/ncomms4472
    Species: Human
    Sample type: Conditioned Media (Cancer Associated Fibroblasts and Lung cancer stem cells)
  3. Lu Y, Cai Z, Xiao G, Keller ET, et al. Monocyte Chemotactic Protein-1 Mediates Prostate Cancer-Induced Bone Resorption. Cancer Res. 2007; 67(8):3646-3653.
    Species: Human
    Sample type: Conditioned Media (Prostate tumor cell and prostate epithelial Cell-conditioned media supernates)
  4. Low-Marchelli JM, Ardi VC, Vizcarra EA, et al. Twist1 Induces CCL2 and Recruits Macrophages to Promote Angiogenesis. Cancer Res. 2013;73:662-671.
    Species: Human
    Sample type: Conditioned Media (mammary epithelial cells, control and treated)
  5. Jin HJ., Lee HJ., Heo J., et al. Senescence associated MCP-1 secretion is dependent on a decline in BMI1 in human mesenchymal stromal cells. Antioxidants & redox signalling [1523-0864], 2015, epub ahead of print.
    Species: Human
    Sample type: Conditioned Media (Umbilical cord cells)
  6. Jin H., Lee H., Heo J., et al. Senescence-Associated MCP-1 Secretion Is Dependent on a Decline in BMI1 in Human Mesenchymal Stromal Cells. Antioxid Redox Signal. 2016 Mar 20;24(9):471-85. doi: 10.1089/ars.2015.6359.
    Species: Human
    Sample type: Conditioned Media (Umbilical cord MSCs under various treatments)
  7. Ukropec J, Penesova A, Kopkova M, Pura M, Vlcek M,et al.Adipokine Protein Expression Pattern in Growth Hormone Deficiency Predisposes to the Increased Fat Cell Size and the Whole Body Metabolic Derangements. J Clin Endocrinol Metab. 2008;93: 2255-2262
    Species: Human
    Sample type: Tissue Lysate (tissue Lysates)
  8. Ho Ia., Toh H., Ng W., et al. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis. Stem Cells. 2013 Jan;31(1):146-55. doi: 10.1002/stem.1247
    Species: Human
    Sample type: Conditioned Media (Mesenchymal stem cell conditioned media)
  9. Ho I., Toh H., Ng W., et al. Human Bone Marrow?Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis, Stem Cells. 2013 Jan;31(1):146-55. doi: 10.1002/stem.1247.
    Species: Human
    Sample type: Conditioned Media (glioma cells and mesenchymal stem cells )
  10. Liu, D., Steins, A., Klassen, R., et al. Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma. Biomedicines. 2020 Nov; 8(11): 444.
    Species: Human
    Sample type: Conditioned Media (HPS, Hypoxic PS1 supernates)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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