Human Cytokine Array G3

RayBio® G-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.

$540.35  
AAH-CYT-G3-4
+

Antigen Information

Gene Symbols:
ANG | CCL1 | CCL15 | CCL17 | CCL2 | CCL22 | CCL5 | CCL7 | CCL8 | CSF1   View more

Assay Format

Number of Targets Detected:
42
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
ENA-78
(CXCL5)
GCSF
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
I-309
(TCA-3/CCL1)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
IL-8
(CXCL8)
IL-10
IL-12 p40/p70
  • Gene Symbol: IL12A; IL12B
  • Accession #: P29459
IL-13
IL-15
IFN-gamma
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 delta
(CCL15)
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TNF alpha
TNF beta
(TNFSF1B)
EGF
IGF-1
Angiogenin
Oncostatin M
Thrombopoietin
(TPO)
VEGF-A
PDGF-BB
Leptin

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array G3 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Gao Y., Zhou Z., Lu S., et al. Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma. Stem Cells. 2016 Jan 13. doi: 10.1002/stem.2275.
    Species: Human
    Sample type: Conditioned Media (Human MSCs, hepatocellular carcinoma cells and normal human liver cells.)
  2. Ringelstein-Harlev, S., Avivi, I., Fanadka, M., Horowitz, N. A., & Katz, T. (2018). Chronic lymphocytic leukemia cells acquire regulatory B-cell properties in response to TLR9 and CD40 activation. Cancer Immunology, Immunotherapy, 67(5), 739–748. doi:10.1007/s00262-018-2128-x 
    Species: Human
    Sample type: Conditioned media (cell culture media)
  3. Wei FF., Zhang X., Xu Z., Zhang S. New Markers to Explore Spontaneous Bacterial Peritonitis. J Mol Biomark Diagn 2014, 5:6 doi:10.4172/2155-9929.1000200
    Species: Human
    Sample type: Peritoneal Fluid (Ascites fluid from spontaneous bacterial peritonitis patients)
  4. Liu S., Wang X., Lu Y., et al. The combined use of cytokine-induced killer cells and cyclosporine a for the treatment of aplastic anemia in a mouse model. J Interferon Cytokine Res. 2015 May;35(5):401-10. doi: 10.1089/jir.2014.0156.
    Species: Human
    Sample type: Conditioned Media (Cytokine induced killer cells form PBMCs)
  5. Cao, Thanh V., et al. "Dosed myofascial release in three-dimensional bioengineered tendons: effects on human fibroblast hyperplasia, hypertrophy, and cytokine secretion." Journal of Manipulative & Physiological Therapeutics 36.8 (2013): 513-521.
    Species: Human
    Sample type: Conditioned Media (fibroblasts; MYOFASCIAL RELEASE IN THREE-DIMENSIONAL BIOENGINEERED TENDONS)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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