Human Cytokine Array G4000

RayBio® G-Series Human Cytokine Antibody Array 4000 Kit. A combination of Human Cytokine Antibody Array G6, G7, G8, G9, & G10. Detects 274 Human Cytokines. Suitable for all liquid sample types.

$3,277.45
AAH-CYT-G4000-4
+

Antigen Information

Gene Symbols:
ACE2 | ADAM17 | ADIPOQ | AFP | AGER | AGRP | ALCAM | ANG | ANGPT1 | ANGPT2   View more

Assay Format

Number of Targets Detected:
274
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
ACE-2
  • Gene Symbol: ACE2
  • Gene ID: 59272
  • Accession #: Q9BYF1
Adiponectin
(ACRP30)
Activin A
Adipsin
(Complement Factor D)
AgRP
ALCAM
(CD166)
Alpha-fetoprotein
Amphiregulin
Angiogenin
Angiopoietin-1
Angiopoietin-2
ANGPTL4
Axl
CD80
(B7-1)
Beta-2 Microglobulin
BCAM
BCMA
(TNFRSF17)
BDNF
beta IG-H3
bFGF
BLC
(CXCL13)
BMP-4
BMP-5
BMP-6
BMP-7
beta-NGF
Betacellulin
(BTC)
CA125
CA15-3
CA19-9
CA9
Cardiotrophin-1
(CT-1)
Cathepsin S
HCC-1
(CCL14)
6Ckine
(CCL21)
CCL28
(MEC)
CD14
CD23
CD30
(TNFRSF8)
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
CEACAM-1
CK beta 8-1
(CCL23)
CNTF
Cripto-1
  • Gene Symbol: TDGF1
  • Gene ID: 6997
  • Accession #: P13385
CRP
(C-Reactive Protein)
CTACK
(CCL27)
CXCL16
DAN
Decorin
DKK-1
Dkk-3
Dkk-4
CD26
(DPPIV)
DR6
(TNFRSF21)
Dtk
E-Cadherin
EDA-A2
EGF
EGFR
EG-VEGF
(PK1)
ENA-78
(CXCL5)
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
TROP1
(EpCAM)
ErbB2
ErbB3
Erythropoietin R
E-Selectin
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
Fc gamma RIIB/C
(CD32B/C)
Ferritin
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
FLRG
Follistatin
Fractalkine
(CX3CL1)
FSH
Furin
Galectin-7
GCP-2
(CXCL6)
GCSF
GDF-15
GDNF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
Growth Hormone
HB-EGF
HCC-4
(CCL16)
hCG intact
HGF
HVEM
(TNFRSF14)
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
ICAM-2
(CD102)
ICAM-3
(CD50)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IL-1 R2
IL-1 R4
(ST2)
IL-1 R1
IL-10
IL-10 R alpha
IL-10 R beta
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-13 R alpha 2
IL-13 R1
IL-15
IL-16
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-17B
IL-17C
  • Gene Symbol: IL17C
  • Gene ID: 27189
  • Accession #: Q9P0M4
IL-17F
IL-17 RA
IL-18 BP alpha
IL-18 R beta
(AcPL)
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R beta
(CD122)
IL-2 R gamma
(Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-2 R alpha
IL-21 R
IL-22
IL-28A
(IFN-lambda 2)
IL-29
(IFN-lambda 1)
IL-3
IL-31
IL-4
IL-5
IL-5 R alpha
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
IL-9
Insulin
IP-10
(CXCL10)
I-TAC
(CXCL11)
LAP/TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
Leptin
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: 3953
  • Accession #: P48357
LIF
Light
(TNFSF14)
LIMPII
L-Selectin
(CD62L)
Luteinizing hormone
Lymphotactin
(XCL1)
LYVE-1
Marapsin
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
M-CSF R
MDC
(CCL22)
MICA
MICB
MIF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-1
MMP-10
MMP-13
MMP-2
MMP-3
MMP-7
MMP-8
MMP-9
MPIF-1
(CCL23)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
NCAM-1
(CD56)
NGFR
(TNFRSF16)
Nidogen-1
NrCAM
NRG1-beta 1
(HRG1-beta 1)
NT-3
NT-4
Oncostatin M
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
PAI-1
PARC
(CCL18)
PDGF-AA
PDGF R alpha
PDGF R beta
PDGF-AB
PDGF-BB
PECAM-1
(CD31)
PLGF
Platelet factor 4
(CXCL4)
Procalcitonin
Prolactin
  • Gene Symbol: PRL
  • Gene ID: 5617
  • Accession #: P01236
PSA-free
PSA-total
RAGE
RANK
(TNFRSF11A)
RANTES
(CCL5)
Resistin
S100 B
  • Gene Symbol: S100B
  • Gene ID: 6285
  • Accession #: P04271
SAA
(Serum Amyloid A)
SCF
SCF R
(CD117/c-kit)
SDF-1 alpha
(CXCL12 alpha)
SDF-1 beta
(CXCL12 beta)
gp130
Sonic Hedgehog N-Terminal
(Shh-N)
Siglec-5
(CD170)
Siglec-9
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TACE
TARC
(CCL17)
TECK
(CCL25)
TGF beta 2
TGF alpha
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TGF beta 1
Thrombopoietin
(TPO)
Thyroglobulin
Tie-1
Tie-2
TIM-1
(KIM-1)
TIMP-1
TIMP-2
TIMP-4
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R2
(TNFRSF10B/DR5)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
Trappin-2
TREM-1
TSH
TSLP
Ubiquitin+1
uPAR
VCAM-1
(CD106)
VE-Cadherin
(CDH5)
  • Gene Symbol: CDH5
  • Gene ID: 1003
  • Accession #: P33151
VEGF-A
VEGFR2
VEGFR3
VEGF-C
VEGF-D
XEDAR
  • Gene Symbol: EDA2R
  • Gene ID: 60401
  • Accession #: Q9HAV5

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array G4000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Peltzer J., et al. Heterogeneous functions of Perinatal Mesenchymal Stromal Cells require a pre-selection before their banking for clinical use. Stem Cells and Development, Sept 2014, epud ahead of print. doi:10.1089/scd.2014.0327
    Species: Human
    Sample type: Conditioned Media
  2. Yang C.Q., MCP-1 Stimulates MMP-9 Expression via ERK 1/2 and p38 MAPK Signaling Pathways in Human Aortic Smooth Muscle Cells. Cell Physiol Biochem 2014;34:266-276 DOI: 10.1159/000362997
    Species: Human
    Sample type: Tissue Lysate
  3. Wu T., Du Y., Han J., et al., Urinary Angiostatin - a novel marker of renal pathology chronicity in lupus nephritis. Mol Cell Proteomics. 2013 May;12(5):1170-9. doi: 10.1074/mcp.M112.021667.
    Species: Human
    Sample type: Urine
  4. Senst, Christiane, et al. "Prospective dual role of mesenchymal stem cells in breast tumor microenvironment." Breast cancer research and treatment137.1 (2013): 69-79.
    Species: Human
    Sample type: Conditioned Media
  5. Gaberman, Elena, et al. "Mitigation of lethal radiation syndrome in mice by intramuscular injection of 3D cultured adherent human placental stromal cells." PloS one 8.6 (2013): e66549.
    Species: Human
    Sample type: Conditioned Media
  6. Straussman R., Morikawa T., et al. Tumourmicro-environment elicits innate resistance to RAF inhibitors through HGF secretion. Nature. 2012 Jul 26;487(7408):500-4. doi: 10.1038/nature11183.
    Species: Human
    Sample type: Conditioned Media
  7. Vanarsa K, Ye Y, Han J, Xie C, Mohan C, Wu T. Inflammation associated anemia and ferritin as disease markers in SLE. Arthritis Research & Therapy 2012;14(4):R182. doi:10.1186/ar4012.
    Species: Human
    Sample type: Urine
  8. Hong, Celine S., et al. "A computational method for prediction of excretory proteins and application to identification of gastric cancer markers in urine." PLoS One 6.2 (2011): e16875.
    Species: Human
    Sample type: Urine
  9. Lichtenauer, Michael, et al. "Intravenous and intramyocardial injection of apoptotic white blood cell suspensions prevents ventricular remodelling by increasing elastin expression in cardiac scar tissue after myocardial infarction." Basic research in cardiology 106.4 (2011): 645-655.
    Species: Human
    Sample type: Conditioned Media
  10. Hanzu, F. A., et al. "Translational evidence of endothelial damage in obese individuals: inflammatory and prothrombotic responses." Journal of Thrombosis and Haemostasis 9.6 (2011): 1236-1245.
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.