Human Cytokine Array G5

RayBio® G-Series Human Cytokine Antibody Array 5 Kit. Detects 80 Human Cytokines. Suitable for all liquid sample types.

$650.44
AAH-CYT-G5-4
+

Antigen Information

Gene Symbols:
ANG | BDNF | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18 | CCL2 | CCL20   View more

Assay Format

Number of Targets Detected:
80
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Angiogenin
BDNF
BLC
(CXCL13)
CK beta 8-1
(CCL23)
EGF
ENA-78
(CXCL5)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GM-CSF
GRO alpha
(CXCL1)
GRO alpha/beta/gamma
HGF
I-309
(TCA-3/CCL1)
IFN-gamma
IGF-1
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
IL-8
(CXCL8)
IL-10
IL-12 p40/p70
  • Gene Symbol: IL12A NKSF1
  • Gene ID: 3592
  • Accession #: P29459
IL-13
IL-15
IL-16
IP-10
(CXCL10)
Leptin
LIF
LIGHT
(TNFSF14)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
NAP-2
(PPBP/CXCL7)
NT-3
NT-4
Oncostatin M
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-BB
PLGF
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TGF beta 2
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
Thrombopoietin
(TPO)
TIMP-1
TIMP-2
TNF alpha
TNF beta
(TNFSF1B)
VEGF-A

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array G5 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Markus W., Markus S., Simone R., et al. Characterization of sulfur mustard resistant keratinocyte cell line HaCaT/SM. Toxicol Lett. 2016 Feb 26;244:49-55. doi: 10.1016/j.toxlet.2015.10.001.
    Species: Human
    Sample type: Conditioned Media
  2. Ma D., Jua JE., Lim WK., et al. In vitro characterization of human hair follicle dermal sheath mesenchymal stromal cells and their potential in enhancing diabetic wound healing. Cytotherapy. 2015 Aug;17(8):1036-51. doi: 10.1016/j.jcyt.2015.04.001.
    Species: Human
    Sample type: Conditioned Media
  3. Chen H., Shi B., Feng X., et al. Leptin and Neutrophil-Activating Peptide 2 Promote Mesenchymal Stem Cell Senescence Through Activation of the Phosphatidylinositol 3-Kinase/Akt Pathway in Patients With Systemic Lupus Erythematosus. Arthritis Rheumatol. 2015 Sep;67(9):2383-93. doi: 10.1002/art.39196.
    Species: Human
    Sample type: Serum
  4. zhong F., Zhou N., Wu K., et al. A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes. Nucleic Acids Res. 2015 Dec 2;43(21):10474-91. doi: 10.1093/nar/gkv954.
    Species: Human
    Sample type: Cell Lysate
  5. Chollet-Hinton LS., Stuebe AM., Casbas-Hernandez P., et al. Temporal trends in the inflammatory cytokine profile of human breastmilk. Breastfeed Med. 2014 Dec;9(10):530-7. doi: 10.1089/bfm.2014.0043.
    Species: Human
    Sample type: Milk/Colostrum
  6. Zhen A., Krutzik S., Levin B., et al. CD4 ligation on human blood monocytes triggers macrophage differentiation and enhances HIV infection. Journal of Virology published online ahead of print on 18 June 2014. doi:10.1128/JVI.00616-14
    Species: Human
    Sample type: Conditioned Media
  7. Rath BH, Fair JM, Jamal M, Camphausen K, Tofilon PJ (2013) Astrocytes Enhance the Invasion Potential of Glioblastoma Stem-Like Cells. PLoS ONE 8(1):e54752. doi:10.1371/journal.pone.0054752
    Species: Human
    Sample type: Conditioned Media
  8. Choi, Ji Suk, et al. "Full-thickness skin wound healing using human placenta-derived extracellular matrix containing bioactive molecules." Tissue Engineering Part A 19.3-4 (2012): 329-339.
    Species: Human
    Sample type: Tissue Lysate
  9. Stewart DA, Yang Y, Makowski L, Troester MA. Basal-like breast cancer cells induce phenotypic and genomic changes in macrophages. Molecular cancer research?: MCR 2012;10(6):727-738. doi:10.1158/1541-7786.MCR-11-0604.
    Species: Human
    Sample type: Conditioned Media
  10. Smalley-Freed W., et al. Adenoma Formation following Limited Ablation of p120-Catenin in the Mouse Intestine. PLOS One Published: May 17, 2011 DOI:10.1371/journal.pone.0019880
    Species: Human
    Sample type: Conditioned Media
I used this array in HM2 ATCC cell line co-culture supernatant, the protocol is easily to follow.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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