Human Cytokine Array G6

RayBio® G-Series Human Cytokine Antibody Array 6 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

$650.44
AAH-CYT-G6-4
+

Antigen Information

Gene Symbols:
ANG | BDNF | BMP4 | BMP6 | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18   View more

Assay Format

Number of Targets Detected:
60
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Angiogenin
BDNF
BLC
(CXCL13)
BMP-4
BMP-6
Ck beta 8-1
(CCL23)
CNTF
EGF
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
FGF-6
Angiostatin
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GDNF
GM-CSF
I-309
(TCA-3/CCL1)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-4
IGF-1
IL-10
IL-13
IL-15
IL-16
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
Leptin
Light
(TNFSF14)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
NAP-2
(PPBP/CXCL7)
NT-3
PARC
(CCL18)
PDGF-BB
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TNF alpha
TNF beta
(TNFSF1B)

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Human Cytokine Antibody Array G6 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Song R-h., Qiu Q., Xuan W., et al. Differential cytokine expression detected by protein microarray screening in peripheral blood of patients with refractory Graves’ disease. Clinical Endocrinology, epub ahead of print, 2015. DOI: 10.1111/cen.12778
    Species: Human
    Sample type: Plasma
  2. Xin J., Ding W., Hao S., et al. Human bone marrow mesenchymal stem cell-derived hepatocytes express tissue inhibitor of metalloproteinases 4 and follistatin. Liver Int. 2015 Feb 2. doi: 10.1111/liv.12797.
    Species: Human
    Sample type: Conditioned Media
  3. Xin J., Ding W., Hao S., et al. Human bone marrow mesenchymal stemcell derived hepatocytes express tissue inhibitor of metalloproteinases 4 and follistatin. Liver Int. 2015 Feb 2. doi: 10.1111/liv.12797
    Species: Human
    Sample type: Conditioned Media
  4. Kastallari E., Baba-Amer Y., Alonso-Martin S., et al. Pericytes in the myovascular niche promote post-natal myofiber growth and satellite cell quiescence. Development. 2015 Apr 1;142(7):1242-53. doi: 10.1242/dev.115386.
    Species: Human
    Sample type: Conditioned Media
  5. Sun Y., Deng W., Geng L., et al. Mesenchymal stem cells from patients with rheumatoid arthritis display impaired function in inhibiting Th17 cells. J Immunol Res. 2015;2015:284215. doi: 10.1155/2015/284215.
    Species: Human
    Sample type: Conditioned Media
  6. Qin Q., Wang X., Yan N., et al. Differential cytokine expression detected by protein microarray screening in peripheral blood of patients with refractory Graves' disease
    Species: Human
    Sample type: Plasma
  7. Zhang Y., Lin P., Jiang H., et al. Extensive serum biomarker analysis in patients with ST segment elevation myocardial infarction (STEMI). Cytokine. 2015 Dec;76(2):356-62. doi: 10.1016/j.cyto.2015.06.015.
    Species: Human
    Sample type: Serum
  8. Gomez DL, O’Driscoll M, Sheets TP, Hruban RH, Oberholzer J, McGarrigle JJ, et al. (2015) Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate. PLoS ONE 10(8): e0133862. doi:10.1371/journal.pone.0133862
    Species: Human
    Sample type: Conditioned Media
  9. Collado M., Santaella M., Mira-Pascual L., et al. Longitudinal Study of Cytokine Expression, Lipid Profile and Neuronal Growth Factors in Human Breast Milk from Term and Preterm Deliveries. Nutrients. 2015 Oct 19;7(10):8577-91. doi: 10.3390/nu7105415.
    Species: Human
    Sample type: Milk/colostrum
  10. Matte I., Lane D., Laplante C., et al. Ovarian cancer ascites enhance the migration of patient-derived peritoneal mesothelial cells via cMet pathway through HGF-dependent and -independent mechanisms. Int J Cancer. 2014 Dec 6. doi: 10.1002/ijc.29385.
    Species: Human
    Sample type: Peritoneal Fluid

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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