Human Cytokine Array G6

RayBio® G-Series Human Cytokine Antibody Array 6 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

$650.44
AAH-CYT-G6-4
+

Antigen Information

Gene Symbols:
ANG | BDNF | BMP4 | BMP6 | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18   View more

Assay Format

Number of Targets Detected:
60
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Angiogenin
BDNF
BLC
(CXCL13)
BMP-4
BMP-6
Ck beta 8-1
(CCL23)
CNTF
EGF
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
FGF-6
Angiostatin
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GDNF
GM-CSF
I-309
(TCA-3/CCL1)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-4
IGF-1
IL-10
IL-13
IL-15
IL-16
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
Leptin
Light
(TNFSF14)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIG
(CXCL9)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
NAP-2
(PPBP/CXCL7)
NT-3
PARC
(CCL18)
PDGF-BB
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TNF alpha
TNF beta
(TNFSF1B)

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Human Cytokine Antibody Array G6 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Yang C.Q., MCP-1 Stimulates MMP-9 Expression via ERK 1/2 and p38 MAPK Signaling Pathways in Human Aortic Smooth Muscle Cells. Cell Physiol Biochem 2014;34:266-276 DOI: 10.1159/000362997
    Species: Human
    Sample type: Tissue Lysate
  2. Jiang, Weidong, Ruo-Pan Huang, and Ruochun Huang. "Protein Expression Profiling by Antibody Array Analysis with Use of Dried Blood Spot Samples on Filter Paper." Journal of Immunological Methods. ScienceDirect, 25 Nov. 2013. Web.
    Species: Human
    Sample type: Serum
  3. Abdulnour-Nakhoul, Solange M., et al. "Alterations in junctional proteins, inflammatory mediators and extracellular matrix molecules in eosinophilic esophagitis." Clinical immunology 148.2 (2013): 265-278.
    Species: Human
    Sample type: Tissue Lysate
  4. Brunt KR., et al. Ex Vivo Akt/HO-1 Gene Therapy to Human Endothelial Progenitor Cells Enhances Myocardial Infarction Recovery. Cell Transplant. 2012;21(7):1443-61.
    Species: Human
    Sample type: Conditioned Media
  5. Brunt KR., Wu J., Chen Z., et al. Ex vivo Akt/HO-1 gene therapy to human endothelial progenitor cells enhances myocardial infarction recovery. Cell Transplant. 2012;21(7):1443-61.
    Species: Human
    Sample type: Conditioned Media
  6. Greulich S., Maxhera B., et al. Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte Dysfunction. Circulation. 2012; 126: 2324-2334. doi: 10.1161/CIRCULATIONAHA.111.039586
    Species: Human
    Sample type: Conditioned Media
  7. Laberge R-M, Zhou L, Sarantos MR, et al. Glucocorticoids Suppress Selected Components of the Senescence-Associated Secretory Phenotype. Aging Cell 2012;11(4):569-578. doi:10.1111/j.1474-9726.2012.00818.x.
    Species: Human
    Sample type: Conditioned Media
  8. Liu Y., Teoh SH., Chong MS., et al. Vasculogenic and osteogenesis-enhancing potential of human umbilical cord blood endothelial colony-forming cells. Stem Cells. 2012 Sep;30(9):1911-24. doi: 10.1002/stem.1164.
    Species: Human
    Sample type: Conditioned Media
  9. Toiyama Y., Fujikawa H., Kawamura M., et al. Evaluation of CXCL10 as a novel serum marker for predicting liver metastasis and prognosis in colorectal cancer. Int J Oncol. 2012 Feb;40(2):560-6. doi: 10.3892/ijo.2011.1247.
    Species: Human
    Sample type: Serum
  10. Straussman R., Morikawa T., et al. Tumourmicro-environment elicits innate resistance to RAF inhibitors through HGF secretion. Nature. 2012 Jul 26;487(7408):500-4. doi: 10.1038/nature11183.
    Species: Human
    Sample type: Conditioned Media

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Price for all $685.28

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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