Human Cytokine Array G7

RayBio® G-Series Human Cytokine Antibody Array 7 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

AAH-CYT-G7-4
+

Antigen Information

Gene Symbols:
ADIPOQ | AGRP | ANGPT2 | AREG | AXL | BTC | CCL16 | CCL19 | CCL25 | CCL27   View more

Assay Format

Number of Targets Detected:
60
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
AgRP
Angiopoietin-2
Amphiregulin
Axl
bFGF
beta-NGF
Betacellulin
(BTC)
CCL28
(MEC)
CTACK
(CCL27)
Dtk
EGFR
ENA-78
(CXCL5)
Fas
(TNFRSF6/Apo-1)
FGF-4
FGF-9
GCSF
GITR Ligand
(TNFSF18)
GITR
(TNFRSF18)
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
ICAM-1
(CD54)
ICAM-3
(CD50)
IGFBP-3
IGFBP-6
IGF-1 R
IL-1 R4
(ST2)
IL-1 R1
IL-11
IL-12 p40
IL-12 p70
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IL-2 R alpha
IL-6 R
IL-8
(CXCL8)
I-TAC
(CXCL11)
Lymphotactin
(XCL1)
MIF
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-3 beta
(CCL19)
MSP alpha/beta
NT-4
Osteoprotegerin
(TNFRSF11B)
Oncostatin M
PLGF
gp130
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TECK
(CCL25)
TIMP-1
TIMP-2
Thrombopoietin
(TPO)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VEGF-A
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array G7 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Pasquier J., Gosset M., Geyl C., et al. CCL2/CCL5 secreted by the stroma induce IL-6/PYK2 dependent chemoresistance in ovarian cancer. https://www.ncbi.nlm.nih.gov/pubmed/29455640
    Species: Human
    Sample type: Conditioned Media
  2. Li L., Chen L., Zhang W., et al. Serum cytokine profile in patients with breast cancer cytokine. Cytokine, 2016 epub ahead of print
    Species: Human
    Sample type: Serum
  3. Yu G., Okawa H., Okita K., et al. Gingival Fibroblasts as Autologous Feeders for Induced Pluripotent Stem Cells. J Dent Res. 2016 Jan;95(1):110-8. doi: 10.1177/0022034515611602.
    Species: Human
    Sample type: Conditioned Media
  4. Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
    Species: Human
    Sample type: Serum
  5. Markus W., Markus S., Simone R., et al. Characterization of sulfur mustard resistant keratinocyte cell line HaCaT/SM. Toxicol Lett. 2016 Feb 26;244:49-55. doi: 10.1016/j.toxlet.2015.10.001.
    Species: Human
    Sample type: Conditioned Media
  6. Shi D., Zhang J., Zhou Q., et al. Quantitative evaluation of human bone mesenchymal stem cells rescuing fulminant hepatic failure in pigs. Gut. 2016 Feb 16. pii: gutjnl-2015-311146. doi: 10.1136/gutjnl-2015-311146
    Species: Human
    Sample type: Serum
  7. Zhu Z., Tang J., Gang D., et al. Antibody microarray profiling of osteosarcoma cell serum for identifying potential biomarkers. Mol Med Rep. 2015 Jul;12(1):1157-62. doi: 10.3892/mmr.2015.3535.
    Species: Human
    Sample type: Serum
  8. Schwartzbaum J, Seweryn M, Holloman C, Harris R, Handelman SK, Rempala GA, et al. (2015) Association between Prediagnostic Allergy-Related Serum Cytokines and Glioma. PLoS ONE 10(9): e0137503. doi:10.1371/journal.pone.0137503
    Species: Human
    Sample type: Serum
  9. Bermudez MA., Sendon-lago J., Eiro N., et al. Corneal Epithelial Wound Healing and Bactericidal Effect of Conditioned Medium From Human Uterine Cervical Stem Cells. Invest Ophthalmol Vis Sci. 2015 Jan 22;56(2):983-92. doi: 10.1167/iovs.14-15859.
    Species: Human
    Sample type: Conditioned Media
  10. Matsubara K., Matsushita Y., Sakai K., et al. Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity. J Neurosci. 2015 Feb 11;35(6):2452-64. doi: 10.1523/JNEUROSCI.4088-14.2015
    Species: Human
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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