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Human Cytokine Array G7

Name: Human Cytokine Array G7
SKU: AAH-CYT-G7-4
Price: 650.44 USD
Availability: InStock

Citations (37)

RayBio^® G-Series Human Cytokine Antibody Array 7 Kit. Detects 60 Human Cytokines. Suitable for all liquid sample types.

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Manual

SDS

CODE: AAH-CYT-G7-4

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Description
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Antigen Information

Gene Symbols:

ADIPOQ | AGRP | ANGPT2 | AREG | AXL | BTC | CCL16 | CCL19 | CCL25 | CCL27 View more

Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

4 or 8 Sample Kit

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Adiponectin (ACRP30) Gene Symbol: ADIPOQ Gene ID: 9370 Accession #: Q15848 ELISA Kit: RayBio Human Acrp30 ELISA	AgRP Gene Symbol: AgRP Gene ID: 181 Accession #: O00253 ELISA Kit: RayBio Human AgRP ELISA	Angiopoietin-2 Gene Symbol: ANGPT2 Gene ID: 285 Accession #: O15123 ELISA Kit: RayBio Human ANGPT2 ELISA	Amphiregulin Gene Symbol: AREG Gene ID: 374 Accession #: P15514 ELISA Kit: RayBio Human AREG ELISA	Axl Gene Symbol: Axl Gene ID: 558 Accession #: P30530 ELISA Kit: RayBio Human Axl ELISA
bFGF Gene Symbol: FGF2 Gene ID: 2247 Accession #: P09038 ELISA Kit: RayBio Human bFGF ELISA	beta-NGF Gene Symbol: NGF Gene ID: 4803 Accession #: P01138 ELISA Kit: RayBio Human beta-NGF ELISA	Betacellulin (BTC) Gene Symbol: BTC Gene ID: 685 Accession #: P35070 ELISA Kit: RayBio Human BTC ELISA	CCL28 (MEC) Gene Symbol: CCL28 Gene ID: 56477 Accession #: Q9NRJ3 ELISA Kit: RayBio Human CCL28 ELISA	CTACK (CCL27) Gene Symbol: CCL27 Gene ID: 10850 Accession #: Q9Y4X3 ELISA Kit: RayBio Human CTACK ELISA
Dtk Gene Symbol: TYRO3 Gene ID: 7301 Accession #: Q06418 ELISA Kit: RayBio Human Dtk ELISA	EGFR Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human EGFR ELISA	ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA	Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA	FGF-4 Gene Symbol: FGF4 Gene ID: 2249 Accession #: P08620 ELISA Kit: RayBio Human FGF-4 ELISA
FGF-9 Gene Symbol: FGF9 Gene ID: 2254 Accession #: P31371 ELISA Kit: RayBio Human FGF-9 ELISA	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GITR Ligand (TNFSF18) Gene Symbol: TNFSF18 Gene ID: 8995 Accession #: Q9UNG2 ELISA Kit: RayBio Human GITR L ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Gene ID: 8784 Accession #: Q9Y5U5 ELISA Kit: RayBio Human GITR ELISA	GRO alpha/beta/gamma Gene Symbol: CXCL1/CXCL2/CXCL3 Gene ID: 2919/2920/2921 Accession #: P09341/P19875/P19876
GRO alpha (CXCL1) Gene Symbol: CXCL1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA	HCC-4 (CCL16) Gene Symbol: CCL16 Gene ID: 6360 Accession #: O15467 ELISA Kit: RayBio Human HCC-4 ELISA	HGF Gene Symbol: HGF Gene ID: 3082 Accession #: P14210 ELISA Kit: RayBio Human HGF ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	ICAM-3 (CD50) Gene Symbol: ICAM3 Gene ID: 3385 Accession #: P32942 ELISA Kit: RayBio Human ICAM-3 ELISA
IGFBP-3 Gene Symbol: IGFBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Gene ID: 3489 Accession #: P24592 ELISA Kit: RayBio Human IGFBP-6 ELISA	IGF-1 R Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069 ELISA Kit: RayBio Human IGF-1 R ELISA	IL-1 R4 (ST2) Gene Symbol: IL1RL1 Gene ID: 9173 Accession #: Q01638 ELISA Kit: RayBio Human IL-1 R4 ELISA	IL-1 R1 Gene Symbol: IL1R1 Gene ID: 3554 Accession #: P14778 ELISA Kit: RayBio Human IL-1 sRI ELISA
IL-11 Gene Symbol: IL11 Gene ID: 3589 Accession #: P20809 ELISA Kit: RayBio Human IL-11 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552	IL-2 R alpha Gene Symbol: IL2RA Gene ID: 3559 Accession #: P01589 ELISA Kit: RayBio Human IL-2 R alpha ELISA
IL-6 R Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Gene ID: 6373 Accession #: O14625 ELISA Kit: RayBio Human I-TAC ELISA	Lymphotactin (XCL1) Gene Symbol: XCL1 Gene ID: 6375 Accession #: P47992 ELISA Kit: RayBio Human Lympotactin ELISA	MIF Gene Symbol: MIF Gene ID: 4282 Accession #: P14174 ELISA Kit: RayBio Human MIF ELISA
MIP-1 alpha (CCL3) Gene Symbol: CCL3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 Gene ID: 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	MIP-3 beta (CCL19) Gene Symbol: CCL19 Gene ID: 6363 Accession #: Q99731 ELISA Kit: RayBio Human MIP-3 beta ELISA	MSP alpha/beta Gene Symbol: MST1 Gene ID: 4485 Accession #: P26927 ELISA Kit: RayBio Human MSP ELISA	NT-4 Gene Symbol: NTF4 Gene ID: 4909 Accession #: P34130 ELISA Kit: RayBio Human NT-4 ELISA
Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: 4982 Accession #: O00300 ELISA Kit: RayBio Human OPG ELISA	Oncostatin M Gene Symbol: OSM Gene ID: 5008 Accession #: P13725 ELISA Kit: RayBio Human OSM ELISA	PLGF Gene Symbol: PGF Gene ID: 5228 Accession #: P49763 ELISA Kit: RayBio Human PLGF ELISA	gp130 Gene Symbol: Il6st Gene ID: 3572 Accession #: P40189 ELISA Kit: RayBio Human sgp130 ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA
TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA	TECK (CCL25) Gene Symbol: CCL25 Gene ID: 6370 Accession #: O15444 ELISA Kit: RayBio Human TECK ELISA	TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA
TRAIL R3 (TNFRSF10C) Gene Symbol: TNFRSF10C Gene ID: 8794 Accession #: O14798 ELISA Kit: RayBio Human TRAIL R3 ELISA	TRAIL R4 (TNFRSF10D) Gene Symbol: TNFRSF10D Gene ID: 8793 Accession #: Q9UBN6 ELISA Kit: RayBio Human TRAIL R4 ELISA	uPAR Gene Symbol: PLAUR Gene ID: 5329 Accession #: Q03405 ELISA Kit: RayBio Human uPAR ELISA	VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA	VEGF-D Gene Symbol: FIGF Gene ID: 2277 Accession #: O43915 ELISA Kit: RayBio Human VEGF-D ELISA

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array G7 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Manual

SDS

Pasquier J., Gosset M., Geyl C., et al. CCL2/CCL5 secreted by the stroma induce IL-6/PYK2 dependent chemoresistance in ovarian cancer. https://www.ncbi.nlm.nih.gov/pubmed/29455640
Species: Human
Sample type: Conditioned Media
Li L., Chen L., Zhang W., et al. Serum cytokine profile in patients with breast cancer cytokine. Cytokine, 2016 epub ahead of print
Species: Human
Sample type: Serum
Yu G., Okawa H., Okita K., et al. Gingival Fibroblasts as Autologous Feeders for Induced Pluripotent Stem Cells. J Dent Res. 2016 Jan;95(1):110-8. doi: 10.1177/0022034515611602.
Species: Human
Sample type: Conditioned Media
Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905.
Species: Human
Sample type: Serum
Markus W., Markus S., Simone R., et al. Characterization of sulfur mustard resistant keratinocyte cell line HaCaT/SM. Toxicol Lett. 2016 Feb 26;244:49-55. doi: 10.1016/j.toxlet.2015.10.001.
Species: Human
Sample type: Conditioned Media
Shi D., Zhang J., Zhou Q., et al. Quantitative evaluation of human bone mesenchymal stem cells rescuing fulminant hepatic failure in pigs. Gut. 2016 Feb 16. pii: gutjnl-2015-311146. doi: 10.1136/gutjnl-2015-311146
Species: Human
Sample type: Serum
Zhu Z., Tang J., Gang D., et al. Antibody microarray profiling of osteosarcoma cell serum for identifying potential biomarkers. Mol Med Rep. 2015 Jul;12(1):1157-62. doi: 10.3892/mmr.2015.3535.
Species: Human
Sample type: Serum
Schwartzbaum J, Seweryn M, Holloman C, Harris R, Handelman SK, Rempala GA, et al. (2015) Association between Prediagnostic Allergy-Related Serum Cytokines and Glioma. PLoS ONE 10(9): e0137503. doi:10.1371/journal.pone.0137503
Species: Human
Sample type: Serum
Bermudez MA., Sendon-lago J., Eiro N., et al. Corneal Epithelial Wound Healing and Bactericidal Effect of Conditioned Medium From Human Uterine Cervical Stem Cells. Invest Ophthalmol Vis Sci. 2015 Jan 22;56(2):983-92. doi: 10.1167/iovs.14-15859.
Species: Human
Sample type: Conditioned Media
Matsubara K., Matsushita Y., Sakai K., et al. Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity. J Neurosci. 2015 Feb 11;35(6):2452-64. doi: 10.1523/JNEUROSCI.4088-14.2015
Species: Human
Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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Human Cytokine Array G7

Antigen Information

Assay Format

Product Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Analysis Tool Bundle Discount

Usage

G-Series Testing Service G-Series Testing Service

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