Human Cytokine Array G8

RayBio® G-Series Human Cytokine Antibody Array 8 Kit. Detects 54 Human Cytokines. Suitable for all liquid sample types.

$1,105.75  
AAH-CYT-G8-8
+

Assay Format

Number of Targets Detected:
54
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Protein Information

Gene Symbols:
ALCAM | BMP5 | BMP7 | CCL23 | CD14 | CD80 | CDH5 | CSF1R | CTF1 | CXCL10   View more

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Activin A
ALCAM
(CD166)
CD80
(B7-1)
BMP-5
BMP-7
Cardiotrophin-1
(CT-1)
CD14
CXCL16
DR6
(TNFRSF21)
Endoglin
(CD105)
ErbB3
E-Selectin
Fas Ligand
(TNFSF6)
ICAM-2
(CD102)
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IL-1 R2
IL-10 R beta
IL-13 R alpha 2
IL-18 BP alpha
IL-18 R beta
(AcPL)
MMP-3
IL-2 R beta
(CD122)
IL-2 R gamma
(Common gamma Chain)
  • Gene Symbol: IL2RG
  • Gene ID: 3561
  • Accession #: P31785
IL-21 R
IL-5 R alpha
IL-9
IP-10
(CXCL10)
LAP/TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 7040
  • Accession #: P01137
Leptin R
  • Gene Symbol: LEPR
  • Gene ID: 3953
  • Accession #: P48357
LIF
L-Selectin
(CD62L)
M-CSF R
MMP-1
MMP-13
MMP-9
MPIF-1
(CCL23)
NGFR
(TNFRSF16)
PDGF-AA
PDGF-AB
PDGF R alpha
PDGF R beta
PECAM-1
(CD31)
Prolactin
  • Gene Symbol: PRL
  • Gene ID: 5617
  • Accession #: P01236
SCF R
(CD117/c-kit)
SDF-1 beta
(CXCL12 beta)
Siglec-5
(CD170)
TGF alpha
TGF beta 2
Tie-1
Tie-2
TIMP-4
VE-Cadherin
(CDH5)
  • Gene Symbol: CDH5
  • Gene ID: 1003
  • Accession #: P33151
VEGFR2
VEGFR3

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array G8 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Straussman R., Morikawa T., et al. Tumourmicro-environment elicits innate resistance to RAF inhibitors through HGF secretion. Nature. 2012 Jul 26;487(7408):500-4. doi: 10.1038/nature11183.
    Species: Human
    Sample type: Conditioned Media (Stromal Cell Lines)
  2. Greulich S., Maxhera B., et al. Secretory Products From Epicardial Adipose Tissue of Patients With Type 2 Diabetes Mellitus Induce Cardiomyocyte Dysfunction. Circulation. 2012; 126: 2324-2334. doi: 10.1161/CIRCULATIONAHA.111.039586
    Species: Human
    Sample type: Conditioned Media (Adipose tissue biopsies)
  3. Shi D., Zhang J., Zhou Q., et al. Quantitative evaluation of human bone mesenchymal stem cells rescuing fulminant hepatic failure in pigs. Gut. 2016 Feb 16. pii: gutjnl-2015-311146. doi: 10.1136/gutjnl-2015-311146
    Species: Human
    Sample type: Serum (Officially a pig stem cell transplant model of human Stem Cells)
  4. Ko TM., Kuo HC., Chang JS., et al. CXCL10/IP-10 is a Biomarker and Mediator for Kawasaki Disease. Circ Res. 2015 Feb 27;116(5):876-83. doi: 10.1161/CIRCRESAHA.116.305834.
    Species: Human
    Sample type: Plasma (Kawasaki's Disease patients)
  5. Park JE., Hypoxic Tumor Cell Modulates Its Microenvironment to Enhance Angiogenic and Metastatic Potential by Secretion of Proteins and Exosomes. Molecular & Cellular Proteomics, June 1, 9, 1085-1099.
    Species: Human
    Sample type: Conditioned Media
  6. Matsubara K., Matsushita Y., Sakai K., et al. Secreted Ectodomain of Sialic Acid-Binding Ig-Like Lectin-9 and Monocyte Chemoattractant Protein-1 Promote Recovery after Rat Spinal Cord Injury by Altering Macrophage Polarity. J Neurosci. 2015 Feb 11;35(6):2452-64. doi: 10.1523/JNEUROSCI.4088-14.2015
    Species: Human
    Sample type: Conditioned Media (Bone marrow stem cells and stem cells from human dental pulp)
  7. Liu Y., Teoh SH., Chong MS., et al. Vasculogenic and osteogenesis-enhancing potential of human umbilical cord blood endothelial colony-forming cells. Stem Cells. 2012 Sep;30(9):1911-24. doi: 10.1002/stem.1164.
    Species: Human
    Sample type: Conditioned Media (Endothelial Colony Forming Cells)
  8. Kocaoemer A, Kern S, Kluter H, Bieback K, Human AB Serum and Thrombin-Activated Platelet-Rich Plasma Are Suitable Alternatives to Fetal Calf Serum for the Expansion of Mesenchymal Stem Cells from Adipose Tissue. Stem Cell. 2007;25:1270-1278.
    Species: Human
    Sample type: Serum (Human serum and platelet-rich plasma)
  9. Yang C.Q., MCP-1 Stimulates MMP-9 Expression via ERK 1/2 and p38 MAPK Signaling Pathways in Human Aortic Smooth Muscle Cells. Cell Physiol Biochem 2014;34:266-276 DOI: 10.1159/000362997
    Species: Human
    Sample type: Tissue Lysate (Abdominal Aortic Artery samples)
  10. Dumortier J, Streblow DN, Moses AV, Jacobs JM, Kreklywich CN, et al. Human Cytomegalovirus Secretome Contains Factors That Induce Angiogenesis and Wound Healing. J Virol. 2008; 82(13):6524-6655.
    Species: Human
    Sample type: Conditioned Media (Dermal fibroblast-conditioned media)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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