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Human Cytokine Array GS440

G-Series Human Cytokine Array 440 Kit. A combination of 11 non-overlapping arrays to measure the relative expression levels of 440 human cytokines. Suitable for all liquid sample types.

Old price: $26,357.76
$21,086.21  
You save: $5,271.55 (20%)
GSH-CAA-440-4
+

Antigen Information

Gene Symbols:
ACAN | ACE2 | ADAM12 | ADAM17 | ADAM8 | ADAM9 | ADAMTS13 | ADIPOQ | AFP | AGRP   View more

Assay Format

Number of Targets Detected:
440
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 &mirco;L per array).

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

2B4 (CD244), 4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), ACE-2, Activin A, ADAM-12, ADAM-8, ADAM-9, ADAMTS-13, Adiponectin (ACRP30), Adipsin (Complement Factor D), aFGF (FGF-1), Aggrecan, AgRP, Albumin, ALCAM (CD166), ALK-6 (BMPR-IB), Alpha-fetoprotein, AMICA, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensinogen (SERPINA8), ANGPTL3, ANGPTL4, APRIL, AXL, B7-H1 (CD274), B7-H3 (CD276), BAFF, BCAM, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, Betacellulin (BTC), beta-NGF, bFGF, BLC (CXCL13), BMP-2, BMP-4, BMP-5, BMP-7, BMP-9, BMPR-IA (ALK-3), BMPR-II, C5a, CA125, CA15-3, CA19-9, CA9, Cadherin-11, Cadherin-13, Cadherin-4, Cathepsin B (Pro-Cathepsin B), Cathepsin L, Cathepsin S, CCL28 (MEC), CD14, CD147 (EMMPRIN), CD155 (PVR), CD163, CD200, CD229 (SLAMF3), CD23, CD26 (DPPIV), CD27 (TNFRSF7), CD30 (TNFRSF8), CD40 Ligand (TNFSF5), CD40 (TNFRSF5), CD48 (SLAMF2), CD58 (LFA-3), CD6, CD80 (B7-1), CD84 (SLAMF5), CD97, CD99, CEA (CEACAM-5), CEA (CEACAM-5), CEACAM-1, Chemerin, CHI3L1, CHL-1 (L1CAM-2), Ck beta 8-1 (CCL23/MPIF-1), Clusterin, CNTF, Coagulation Factor XIV (Protein C), Contactin-2 (TAG1), Cripto-1, CRP (C-Reactive Protein), CRTAM, CTACK (CCL27), CTLA-4 (CD152), CXCL14 (BRAK), CXCL16, Cystatin A, Cystatin B, Cystatin C, Cystatin E/M, DAN, DcR3, Decorin, Desmoglein-2, DKK-1, DKK-3, DKK-4, DLL1, DNAM-1 (CD226), DR3 (TNFRSF25), DR6 (TNFRSF21), Dtk, E-Cadherin, EDA-A2, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), ErbB2, ErbB3, ErbB4 (HER4), Erythropoietin R, ESAM, E-Selectin, FABP2, FAP, Fas Ligand (TNFSF6), Fas (TNFRSF6/Apo-1), Fc gamma RIIB/C (CD32B/C), Ferritin, Fetuin A, FGF-19, FGF-21, FGF-4, FGF-6, FGF-7 (KGF), FGF-9, FLRG, Flt-3 Ligand, FOLH1 (PSMA), Follistatin, Follistatin-like 1, FOLR1, Fractalkine (CX3CL1), FSH, Furin, Galectin-1, Galectin-2, Galectin-3, Galectin-7, Galectin-9, Gas 1, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, GCSF R (CD114), GDF-15, GDNF, GITR Ligand (TNFSF18), GITR (TNFRSF18), GM-CSF, gp130 (sgp130), Granulysin (NKG5/LAG-2), GRO alpha/beta/gamma, GRO alpha (CXCL1), Growth Hormone, HAI-2, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), hCG beta, HGF, HGFR, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), ICOS, IFN-alpha / beta R2, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGF-2 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R3 (IL-1 R Acp), IL-1 R4 (ST2), IL-1 R5 (IL-18 R alpha), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 1 (IL-13 R1), IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17A, IL-17B, IL-17C, IL-17E (IL-25), IL-17F, IL-18, IL-18 BP alpha, IL-18 R beta (AcPL), IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-20, IL-21, IL-21 R, IL-23, IL-24, IL-27, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-3, IL-31, IL-32 alpha, IL-33 (IL-1 F11), IL-34, IL-36 alpha (IL-1 F6), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-36 Ra (IL-1 F5), IL-37 (IL-1 F7), IL-38 (IL-1 F10), IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), JAM-A (CD321/F11R), JAM-B (VE-JAM/CD322), Kallikrein 14, Kallikrein 5, LAG-3, LAP/TGF beta 1 (TGF beta 1), Layilin, LDL R, Legumain, Leptin, Leptin R, LIF, LIGHT (TNFSF14), LIMPII, Lipocalin-2 (NGAL), LOX-1, LRIG3, LRP-6, L-Selectin (CD62L), Lymphotactin (XCL1), LYVE-1, Marapsin, MBL, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MEPE (OF45), Mer, MICA, MICB, Midkine, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NCAM-1 (CD56), Nectin-4, Neprilysin, NGFR (TNFRSF16), Nidogen-1, Notch-1, NOV (CCN3), NrCAM, NRG1-beta 1 (HRG1-beta 1), NSE, NT-3, NT-4, Oncostatin M, Osteoactivin (GPNMB), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PAI-1, PARC (CCL18), P-Cadherin, PD-1, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, Pentraxin-3 (TSG-14), Pepsinogen 1, Periostin, Persephin, PGRPs, Platelet Factor 4 (CXCL4), PLGF, Pref-1 (DLK-1/FA1), Procalcitonin, Prolactin, Prostasin, PSA-free, RAGE, RANK (TNFRSF11A), RANTES (CCL5), RBP4, Renin, Resistin, RGM-B, ROBO3, S100 A8, SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), SERPINA4, sFRP-3, SIGIRR, Siglec-10, Siglec-5 (CD170), Siglec-7 (CD328), Siglec-9, SLAM (CD150), Sonic Hedgehog N-Terminal (Shh-N), SOST, SP-D, Syndecan-1, Syndecan-3, Syndecan-4, TACE, TACI (TNFRSF13B), TARC (CCL17), TECK (CCL25), Testican 2 (SPOCK2), TFPI, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta R3, Thrombomodulin, Thrombopoietin (TPO), Thrombospondin 1, Thrombospondin 2, Thrombospondin 5 (COMP), Thyroglobulin, Tie-1, Tie-2, TIM-1 (KIM-1), TIM-3 (KIM-3), TIMP-1, TIMP-2, TIMP-4, TLR2, TLR4, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRAIL R1 (TNFRSF10A/DR4), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRAIL (TNFSF10), TRANCE (TNFSF11), Transferrin, Transferrin, Trappin-2, TREM-1, TROP1 (EpCAM), Troponin I, TSH, TSLP, TWEAK (TNFSF12), ULBP-1, ULBP-2 (ALCAN-alpha/N2DL2), uPA, uPAR, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, WIF-1, WISP-1 (CCN4), XEDAR

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming Biological Process
  • Biomarker Validation
  • Validation of Antibody Array Results
  • Quantitative Protein Detection
  • Establishing Normal Range
Kit Components
  • Human Cytokine Array Q440 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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