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Human Cytokine Array Q1

Quantibody® Human Cytokine Array 1 Kit. Detects 20 Human Cytokines. Suitable for all liquid sample types.

Old price: $1,272.96
$1,018.37  
You save: $254.59 (20%)
QAH-CYT-1-2
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Antigen Information

Gene Symbols:
CCL2 | CCL3 | CCL4 | CCL5 | CSF2 | CXCL1 | CXCL2 | CXCL3 | CXCL8 | IFNG   View more

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
GM-CSF
GRO alpha/beta/gamma
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p70
IL-13
IL-2
IL-4
IL-5
IL-6
IL-8
(CXCL8)
MCP-1
(CCL2)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MMP-9
RANTES
(CCL5)
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human Cytokine Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Kränkel, Nicolle, et al. "Novel insights into the critical role of bradykinin and the kinin B2 receptor for vascular recruitment of circulating endothelial repair-promoting mononuclear cell subsets: alterations in patients with coronary disease." Circulation (2012): CIRCULATIONAHA-112.
    Species: Human
    Sample type: Conditioned Media (HAEC cultured for 24 h together with adhering PBMC subpopulations )
  2. Yang J., Fang P., Yu D., et al. Chronic Kidney Disease Induces Inflammatory CD40+ Monocyte Differentiation via Homocysteine Elevation and DNA Hypomethylation. Circ Res. 2016 Nov 11;119(11):1226-1241.
    Species: Human
    Sample type: Serum (Cardiovascular Disease and Kidney Disease Patients)
  3. Chen S., Zha X., Shi L., et al. Upregulated TCR? improves cytokine secretion in T cells from patients with AML. J Hematol Oncol. 2015; 8: 72. Published online 2015 Jun 18. doi: 10.1186/s13045-015-0170-0
    Species: Human
    Sample type: Conditioned Media (TCR zzeta forced expreession in AML T cell patients.)
  4. Sharma M., Anderson S., Schoop R., Hudson J., Induction of multiple pro-inflammatory cytokines by respiratory viruses andreversal by standardized Echinacea, a potent antiviral herbal extract. Antiviral Research 83 (2009) 165-170
    Species: Human
    Sample type: Conditioned Media (MDCK, Vero, Hela, A549, BEAS, cell types)
  5. Lönne, M., Lavrentieva, A., Walter, J. G., & Kasper, C. (2013). Analysis of oxygen-dependent cytokine expression in human mesenchymal stem cells derived from umbilical cord. Cell and tissue research, 353(1), 117-122.
    Species: Human
    Sample type: Cell Lysate (Human MSC were isolated from UCs of three different fullterm deliveries under various O2 conditions)
  6. Kellar, Kathryn L., et al. "Multiple cytokines are released when blood from patients with tuberculosis is stimulated with Mycobacterium tuberculosis antigens." PLoS One 6.11 (2011): e26545.
    Species: Human
    Sample type: Plasma (Blood from Patients with Tuberculosis Is Stimulated with Mycobacterium tuberculosis Antigens)
  7. Chen T., Li H., Lin J., et al. Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array. Tuberculosis (Edinb). 2016 Mar;97:108-17. doi: 10.1016/j.tube.2015.12.007.
    Species: Human
    Sample type: Serum (Tuberculosis patients)
  8. Sharma, M., et al. "The potential use of Echinacea in acne: control of Propionibacterium acnes growth and inflammation." Phytotherapy Research25.4 (2011): 517-521.
    Species: Human
    Sample type: Conditioned Media (BEAS-2B cells, A-549 cells, and Human skin fibroblasts)
  9. Wu H., et al. Upregulation of innate immune responses in a T cell/histiocyte-rich large B cell lymphoma patient with significant autoimmune disorders mimicking systemic lupus erythematosus. Annals of Hematology (2014) 93:353-354
    Species: Human
    Sample type: Plasma (R-CHOP treated samples)
  10. Zhan Y., Zou S., Hua F., Li F., Ji L., Wang W., Ye Y., Sun L., Chen H., Cheng Y. High-dose dexamethasone modulates serum cytokine profile in patients with primary immune thrombocytopenia. Immunology Letters Volume 160, Issue 1, July 2014, Pages 33-38
    Species: Human
    Sample type: Serum (Human Serum)
This array, like their other Q-type products, worked well, was easy to use, and provided a lot of data for my samples at a great cost relative to competitors. I successfully ran supernatants from functional engineered lung tissues and cancer cell lines. The data analysis by the manufacturer was quick as always. When the manufacturer detected an error in the readout, and correctly identified it as too little incubation with the fluorescent antibody, they quickly and at no cost resolved the issue for me. Would definitely recommend this product.

Columbia University
It was the first time we try this kit, I use cells supernatant as sample, and it worked pretty well.

Pros:
1. You can run a lot of samples at the time or just few, and the processing time is basically the same.

2. You don't need a lot of sample volume.

3. The price per sample is pretty competitive compared with other facilities or companies.

Cons:

1. It takes a considerable amount of time to prepare the slides, run them an analyze it.

2. The washing time is uncomfortable for your hands/fingers since it is not as big as plate and is also kind of sharpie.

3. It is definitely time consuming to place the white grid in all the dots at the end of the running in order to analyze it, how ever I'm not sure if this is related with getting a defective set of slides, because in all my slides I got the same raw pretty uneven compared with the rest of them.

4. Again I don't know if I got a kit with defective slides, but all of them had a green mark in a corner of each slide (handwriting look like) that interfered with the signal of the sample close by that.

Stanford University
Received results fairly quickly. Easy to use. Used conditioned media from cultured cells

Houston Methodist Research Institute
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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