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Home/Search results/Products/Arrays/Antibody Arrays/Quantibody: Quantitative Multiplex ELISA Arrays/Cytokine Arrays/Human Cytokine Array Q11

Manual / SDS

Description
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Files

Antigen Information

Gene Symbols:

Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
ALK-1 Gene Symbol: SLPI WAP4 WFDC4 Gene ID: 6590 Accession #: P03973 ELISA Kit: RayBio Human ALK-1 ELISA	B7-H2 (ICOS Ligand) Gene Symbol: ICOSLG B7H2 B7RP1 ICOSL KIAA0653 Gene ID: 102723996; 23308 Accession #: O75144 ELISA Kit: RayBio Human B7-H2 ELISA	BLAME (SLAMF8) Gene Symbol: SLAMF8 BLAME Gene ID: 56833 Accession #: Q9P0V8 ELISA Kit: RayBio Human BLAME ELISA	BMP-8 Gene Symbol: BMP8A Gene ID: 353500 Accession #: Q7Z5Y6 ELISA Kit: RayBio Human BMP-8 ELISA	CD28 Gene Symbol: CD28 Gene ID: 940 Accession #: P10747 ELISA Kit: RayBio Human CD28 ELISA
Common beta Chain Gene Symbol: CSF2RB IL3RB IL5RB Gene ID: 1439 Accession #: P32927 ELISA Kit: RayBio Human Common beta Chain ELISA	Contactin-1 Gene Symbol: CNTN1 Gene ID: 1272 Accession #: Q12860 ELISA Kit: RayBio Human Contactin-1 ELISA	Desmoglein-1 Gene Symbol: DSG1 CDHF4 Gene ID: 1828 Accession #: Q02413 ELISA Kit: RayBio Human Desmoglein-1 ELISA	Desmoglein-3 Gene Symbol: DSG3 CDHF6 Gene ID: 1830 Accession #: P32926 ELISA Kit: RayBio Human Desmoglein-3 ELISA	EDAR Gene Symbol: EDAR DL Gene ID: 10913 Accession #: Q9UNE0
EphA1 Gene Symbol: EPHA1 EPH EPHT EPHT1 Gene ID: 2041 Accession #: P21709 ELISA Kit: RayBio Human EphA1 ELISA	EphB6 Gene Symbol: EPHB6 Gene ID: 2051 Accession #: O15197 ELISA Kit: RayBio Human EphB6 ELISA	Ephrin-B3 Gene Symbol: EFNB3 EPLG8 LERK8 Gene ID: 1949 Accession #: Q15768 ELISA Kit: RayBio Human Ephrin-B3 ELISA	Epiregulin Gene Symbol: EREG Gene ID: 2069 Accession #: O14944 ELISA Kit: RayBio Human Epiregulin ELISA	FGF-12 Gene Symbol: FGF12 FGF12B FHF1 Gene ID: 2257 Accession #: P61328 ELISA Kit: RayBio Human FGF-12 ELISA
FGF-17 Gene Symbol: FGF17 UNQ161/PRO187 Gene ID: 8822 Accession #: O60258 ELISA Kit: RayBio Human FGF-17 ELISA	FOLR2 Gene Symbol: FOLR2 Gene ID: 2350 Accession #: P14207 ELISA Kit: RayBio Human FOLR2 ELISA	Galectin-8 Gene Symbol: LGALS8 Gene ID: 3964 Accession #: O00214 ELISA Kit: RayBio Human Galectin-8 ELISA	Glypican 1 Gene Symbol: GPC1 Gene ID: 2817 Accession #: P35052 ELISA Kit: RayBio Human Glypican 1 ELISA	Glypican 5 Gene Symbol: GPC5 Gene ID: 2262 Accession #: P78333 ELISA Kit: RayBio Human Glypican 5 ELISA
Growth Hormone R Gene Symbol: GHR Gene ID: 2690 Accession #: P10912 ELISA Kit: RayBio Human Growth Hormone R ELISA	IFN-gamma R1 Gene Symbol: IFNGR1 Gene ID: 3459 Accession #: P15260 ELISA Kit: RayBio Human IFN-gamma R1 ELISA	IL-22 R alpha 1 Gene Symbol: IL22RA1 IL22R Gene ID: 58985 Accession #: Q8N6P7 ELISA Kit: RayBio Human IL-22 R alpha 1 ELISA	IL-22BP Gene Symbol: IL22RA2 UNQ5793/PRO19598/PRO19822 Gene ID: 116379 Accession #: Q969J5 ELISA Kit: RayBio Human IL-22BP ELISA	IL-23 R Gene Symbol: IL23R Gene ID: 149233 Accession #: Q5VWK5 ELISA Kit: RayBio Human IL-23 R ELISA
IL-31 RA Gene Symbol: IL31RA CRL3 GPL UNQ6368/PRO21073/PRO21384 Gene ID: 133396 Accession #: Q8NI17 ELISA Kit: RayBio Human IL-31 RA ELISA	IL-7 R alpha (CD127) Gene Symbol: IL7R Gene ID: 3575 Accession #: P16871 ELISA Kit: RayBio Human IL-7 R alpha ELISA	Integrin alpha 5 Gene Symbol: ITGA5 FNRA Gene ID: 3678 Accession #: P08648 ELISA Kit: RayBio Human Integrin alpha 5 ELISA	MDM2 Gene Symbol: MDM2 Gene ID: 4193 Accession #: Q00987 ELISA Kit: RayBio Human MDM2 ELISA	Nectin-1 Gene Symbol: PVRL1 HVEC PRR1 Gene ID: 5818 Accession #: Q15223 ELISA Kit: RayBio Human Nectin-1 ELISA
NKp30 Gene Symbol: NCR3 1C7 LY117 Gene ID: 259197 Accession #: O14931 ELISA Kit: RayBio Human NKp30 ELISA	Nogo Receptor (Reticulon-4 Receptor) Gene Symbol: RTN4R NOGOR UNQ330/PRO526 Gene ID: 65078 Accession #: Q9BZR6 ELISA Kit: RayBio Human Nogo Receptor ELISA	Notch-3 Gene Symbol: NOTCH3 Gene ID: 4854 Accession #: Q9UM47 ELISA Kit: RayBio Human Notch-3 ELISA	Oncostatin M R beta (IL-31 R beta) Gene Symbol: OSMR OSMRB Gene ID: 9180 Accession #: Q99650 ELISA Kit: RayBio Human Oncostatin M R beta ELISA	Prolactin R Gene Symbol: PRLR Gene ID: 5618 Accession #: P16471 ELISA Kit: RayBio Human Prolactin R ELISA
RELT (TNFRSF19L) Gene Symbol: RELT TNFRSF19L Gene ID: 84957 Accession #: Q969Z4 ELISA Kit: RayBio Human RELT ELISA	RYK Gene Symbol: RYK JTK5A Gene ID: 6259 Accession #: P34925 ELISA Kit: RayBio Human Phosphotyrosine RYK ELISA	Semaphorin 6D Gene Symbol: SEMA6D KIAA1479 Gene ID: 80031 Accession #: Q8NFY4 ELISA Kit: RayBio Human Semaphorin 6D ELISA	Semaphorin 7A Gene Symbol: SEMA7A CD108 SEMAL Gene ID: 8482 Accession #: O75326 ELISA Kit: RayBio Human Semaphorin 7A ELISA	Siglec-11 Gene Symbol: SIGLEC11 UNQ9222/PRO28718 Gene ID: 114132 Accession #: Q96RL6 ELISA Kit: RayBio Human Siglec-11 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Cytokine Array Q11 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.