Human DNA Damage Response Array C1

RayBio® C-Series Human DNA Damage Response Antibody Array 1 for the semi-quantitative detection of 27 Human proteins in cell and tissue lysates.

$588.60  
AAH-DDR-1-2
+

Assay Format

Number of Targets Detected:
27
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
ABL1 | APEX1 | ATR | BRCA1 | BRCA2 | CCNB1 | CDC25A | CDK1 | CDK7 | CDKN1A   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
APE1
(APEX1)
ATR
  • Gene Symbol: ATR
  • Gene ID: 545
  • Accession #: Q13535
  • ELISA Kit: RayBio
BRCA1
  • Gene Symbol: BRCA1
  • Gene ID: 672
  • Accession #: P38398
  • ELISA Kit: RayBio
BRCA2
  • Gene Symbol: BRCA2
  • Gene ID: 675
  • Accession #: P51587
  • ELISA Kit: RayBio
c-Abl
  • Gene Symbol: ABL1
  • Gene ID: 25
  • Accession #: P00519
  • ELISA Kit: RayBio
CDC25A
  • Gene Symbol: CDC25A
  • Gene ID: 993
  • Accession #: P30304
  • ELISA Kit: RayBio
CDK1
  • Gene Symbol: CDK1
  • Gene ID: 983
  • Accession #: P06493
  • ELISA Kit: RayBio
CDK7
  • Gene Symbol: CDK7
  • Gene ID: 1022
  • Accession #: P50613
  • ELISA Kit: RayBio
Chk1
  • Gene Symbol: CHEK1
  • Gene ID: 1111
  • Accession #: O14757
  • ELISA Kit: RayBio
Chk2
  • Gene Symbol: CHEK2
  • Gene ID: 11200
  • Accession #: O96017
  • ELISA Kit: RayBio
Cyclin B1
  • Gene Symbol: CCNB1 CCNB
  • Gene ID: 891
  • Accession #: P14635
  • ELISA Kit: RayBio
DNA-PKcs
  • Gene Symbol: PRKDC
  • Gene ID: 5591
  • Accession #: P78527
  • ELISA Kit: RayBio
ERCC1
  • Gene Symbol: ERCC1
  • Gene ID: 2067
  • Accession #: P07992
  • ELISA Kit: RayBio
GADD153
  • Gene Symbol: DDIT3
  • Gene ID: 1649
  • Accession #: P35638
  • ELISA Kit: RayBio
Ku70
  • Gene Symbol: XRCC6
  • Gene ID: 2547
  • Accession #: P12956
  • ELISA Kit: RayBio
Ku80
  • Gene Symbol: XRCC5
  • Gene ID: 7520
  • Accession #: P13010
  • ELISA Kit: RayBio
MDM2
MGMT
  • Gene Symbol: MGMT
  • Gene ID: 4255
  • Accession #: P16455
  • ELISA Kit: RayBio
MSH2
  • Gene Symbol: MSH2
  • Gene ID: 4436
  • Accession #: P43246
  • ELISA Kit: RayBio
NBS1
  • Gene Symbol: NBN NBS NBS1 P95
  • Gene ID: 4683
  • Accession #: O60934
  • ELISA Kit: RayBio
OPTN
  • Gene Symbol: OPTN
  • Gene ID: 10133
  • Accession #: Q96CV9
  • ELISA Kit: RayBio
p21
  • Gene Symbol: CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1
  • Gene ID: 1026
  • Accession #: P38936
  • ELISA Kit: RayBio
p53
PARP
  • Gene Symbol: PARP1
  • Gene ID: 142
  • Accession #: P09874
  • ELISA Kit: RayBio
PLK1
  • Gene Symbol: PLK1
  • Gene ID: 5347
  • Accession #: P53350
  • ELISA Kit: RayBio
PPM1D
  • Gene Symbol: PPM1D
  • Gene ID: 8493
  • Accession #: O15297
  • ELISA Kit: RayBio
Rad17
  • Gene Symbol: RAD17
  • Gene ID: 5884
  • Accession #: O75943
  • ELISA Kit: RayBio
     

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human DNA Damage Response Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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