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Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Chemiluminescence

Quantitative/Semi-Quantitative:

Semi-Quantitative

Solid Support:

Membrane

Product Specifications

Size:

2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
APE1 (APEX1) Gene ID: 328 Accession #: P27695 ELISA Kit: RayBio Human APE1/APEX1 ELISA	ATR Gene Symbol: ATR Gene ID: 545 Accession #: Q13535 ELISA Kit: RayBio	BRCA1 Gene Symbol: BRCA1 Gene ID: 672 Accession #: P38398 ELISA Kit: RayBio	BRCA2 Gene Symbol: BRCA2 Gene ID: 675 Accession #: P51587 ELISA Kit: RayBio	c-Abl Gene Symbol: ABL1 Gene ID: 25 Accession #: P00519 ELISA Kit: RayBio
CDC25A Gene Symbol: CDC25A Gene ID: 993 Accession #: P30304 ELISA Kit: RayBio	CDK1 Gene Symbol: CDK1 Gene ID: 983 Accession #: P06493 ELISA Kit: RayBio	CDK7 Gene Symbol: CDK7 Gene ID: 1022 Accession #: P50613 ELISA Kit: RayBio	Chk1 Gene Symbol: CHEK1 Gene ID: 1111 Accession #: O14757 ELISA Kit: RayBio	Chk2 Gene Symbol: CHEK2 Gene ID: 11200 Accession #: O96017 ELISA Kit: RayBio
Cyclin B1 Gene Symbol: CCNB1 CCNB Gene ID: 891 Accession #: P14635 ELISA Kit: RayBio	DNA-PKcs Gene Symbol: PRKDC Gene ID: 5591 Accession #: P78527 ELISA Kit: RayBio	ERCC1 Gene Symbol: ERCC1 Gene ID: 2067 Accession #: P07992 ELISA Kit: RayBio	GADD153 Gene Symbol: DDIT3 Gene ID: 1649 Accession #: P35638 ELISA Kit: RayBio	Ku70 Gene Symbol: XRCC6 Gene ID: 2547 Accession #: P12956 ELISA Kit: RayBio
Ku80 Gene Symbol: XRCC5 Gene ID: 7520 Accession #: P13010 ELISA Kit: RayBio	MDM2 Gene Symbol: MDM2 Gene ID: 4193 Accession #: Q00987 ELISA Kit: RayBio Human MDM2 ELISA	MGMT Gene Symbol: MGMT Gene ID: 4255 Accession #: P16455 ELISA Kit: RayBio	MSH2 Gene Symbol: MSH2 Gene ID: 4436 Accession #: P43246 ELISA Kit: RayBio	NBS1 Gene Symbol: NBN NBS NBS1 P95 Gene ID: 4683 Accession #: O60934 ELISA Kit: RayBio
OPTN Gene Symbol: OPTN Gene ID: 10133 Accession #: Q96CV9 ELISA Kit: RayBio	p21 Gene Symbol: CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 Gene ID: 1026 Accession #: P38936 ELISA Kit: RayBio	p53 Gene Symbol: TP53 P53 Gene ID: 7157 Accession #: P04637 ELISA Kit: RayBio Human p53 ELISA	PARP Gene Symbol: PARP1 Gene ID: 142 Accession #: P09874 ELISA Kit: RayBio	PLK1 Gene Symbol: PLK1 Gene ID: 5347 Accession #: P53350 ELISA Kit: RayBio
PPM1D Gene Symbol: PPM1D Gene ID: 8493 Accession #: O15297 ELISA Kit: RayBio	Rad17 Gene Symbol: RAD17 Gene ID: 5884 Accession #: O75943 ELISA Kit: RayBio

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human DNA Damage Response Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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