Human E-Selectin ELISA

RayBio® Human E-Selectin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$402.00  
ELH-Eselectin-1
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Assay Format

Detection Range:
30 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma):
50 - 200 fold
Sensitivity:
30 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P16581
Gene ID:
  • 6401
Gene Symbols:
  • ELAM1
  • SELE
Protein Name & Synonyms:
E-selectin (CD62 antigen-like family member E) (Endothelial leukocyte adhesion molecule 1) (ELAM-1) (Leukocyte-endothelial cell adhesion molecule 2) (LECAM2) (CD antigen CD62E)
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Ko TM., Kuo HC., Chang JS., et al. CXCL10/IP-10 is a Biomarker and Mediator for Kawasaki Disease. Circ Res. 2015 Feb 27;116(5):876-83. doi: 10.1161/CIRCRESAHA.116.305834.
    Species: Human
    Sample type: Plasma (Kawasaki's Disease patients)
  2. Festuccia, C., Mancini, A., Gravina, G. L., Colapietro, A., Vetuschi, A., Pompili, S., … Magnani, J. (2019). Dual CXCR4 and E-Selectin Inhibitor, GMI-1359, Shows Anti-Bone Metastatic Effects and Synergizes with Docetaxel in Prostate Cancer Cell Intraosseous Growth. Cells, 9(1), 32. doi:10.3390/cells9010032 
    Species: Human
    Sample type: Conditioned media (tissue culture)
  3. Morel S, Léveillé P, Samoilenko M, Franco A, England J, Malaquin N, Tu V, Cardin GB, Drouin S, Rodier F, Lippé S, Krajinovic M, Laverdière C, Sinnett D, Lefebvre G, Levy E, Marcil V. Biomarkers of cardiometabolic complications in survivors of childhood ac
    Species: human
    Sample type: Plasma
  4. Farjo KM, Farjo RA, Halsey S, Moiseyev G, Ma J. Retinol-Binding Protein 4 Induces Inflammation in Human Endothelial Cells by an NADPH Oxidase- and Nuclear Factor Kappa B-Dependent and Retinol-Independent Mechanism. Molecular and Cellular Biology 2012;32(24):5103-5115. doi:10.1128/MCB.00820-12.
    Species: Human
    Sample type: Conditioned Media (Human retinal capillary endothelial and human umbilical vein endothelial cells)
  5. Kesebir S, Turan Ç, Süner Ö, Yaylac? ET. Increased ICAM, VCAM, and E-selectin levels in first manic episode. Bipolar Disord 2014: Ahead of Print Oct 2014 DOI:10.1111/bdi.12269
    Species: Human
    Sample type: Plasma (Mania Patients)
  6. Liu, Zhao-Min, et al. "Effect of soy protein and isoflavones on blood pressure and endothelial cytokines: a 6-month randomized controlled trial among postmenopausal women." Journal of hypertension 31.2 (2013): 384-392.
    Species: Human
    Sample type: Serum (postmenopausal women with pre or early diabetes w)
  7. Chao, Pi-Yu, Ya-Ping Huang, and Wen-Bin Hsieh. "Inhibitive effect of purple sweet potato leaf extract and its components on cell adhesion and inflammatory response in human aortic endothelial cells." Cell adhesion & migration 7.2 (2013): 237-245.
    Species: Human
    Sample type: Conditioned Media (effects of pytochemicals and aspirin on the HAECs surface expression)
  8. Sadik N., Mohamed W., Ahmed M. The association of receptor of advanced glycated end products and inflammatory mediators contributes to endothelial dysfunction in a prospective study of acute kidney injury patients with sepsis. Mol Cell Biochem. 2012 Jan;359(1-2):73-81. doi: 10.1007/s11010-011-1001-4.
    Species: Human
    Sample type: Serum (septic acute kidney injury patients)
  9. Siristatidis C., Askoxylaki M., Varounis C., et al. E-selectin, resistin and reactive oxygen species levels in GnRH -agonist and -antagonist protocols in IVF/ICSI: a prospective cohort study. J Assist Reprod Genet. 2015 Jun;32(6):959-67. doi: 10.1007/s10815-015-0489-z.
    Species: Human
    Sample type: Serum (IVF patients undergoing RnGRH treatments)
  10. Lin, Kuan-Hung, et al. "Chlorophyll-related compounds inhibit cell adhesion and inflammation in human aortic cells." Journal of medicinal food 16.10 (2013): 886-898.
    Species: Human
    Sample type: Cell Lysate (Human aortic endothelial cells )
Very sensitive assay system. Highly recommended.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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