Human EGF ELISA

RayBio® Human EGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$756.00  
ELH-EGF-2
+

Assay Format

Detection Range:
1 pg/ml - 200 pg/ml
Recommended Dilution (serum & plasma):
5 - 50 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P01133
Gene ID:
  • 1950
Gene Symbols:
  • EGF
Protein Name & Synonyms:
Pro-epidermal growth factor (EGF) [Cleaved into: Epidermal growth factor (Urogastrone)]
Target Species:
  • Human

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Mause S., Ritzel E., Liehn E., et al. Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury. Circulation . 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473.
    Species: Human
    Sample type: Conditioned Media (HUVEC cells in vascular injury model)
  2. Del Fante, Claudia, et al. "Conditioned Medium Originated From Lethally Irradiated Umbilical Cord Blood-Derived Mononuclear Cells Has Different Pro-Angiogenic Effects Over Mature and Progenitor Endothelial Cells In Vitro." (2013): 1068-1068.
    Species: Human
    Sample type: Conditioned Media (monnuclear cells)
  3. Lim Y., Yoo J., Kim M., et al. GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands. Mol Cancer Ther. 2016 Feb;15(2):251-63. doi: 10.1158/1535-7163.MCT-15-0679
    Species: Human
    Sample type: Conditioned Media (Tumor xenografts)
  4. Groenestege WMT, Thebault S, van der Wijst J, van den Berg D, et al. Impaired basolateral sorting of pro-EGF causes isolated recessive renal hypomagnesemia. J Clin Invest. 2007;117:2260-2267.
    Species: Human
    Sample type: Conditioned Media (Human endothelial kidney (HEK293) and canine kidney (MDCK) cell-conditioned media)
  5. Ryu, Ju Hee, et al. "In vivo fluorescence imaging for cancer diagnosis using receptor-targeted epidermal growth factor-based nanoprobe." Biomaterials 34.36 (2013): 9149-9159.
    Species: Human
    Sample type: Nanomaterials (human EGF and EGF-NP w)
  6. Miyabayashi K, Ijichi H, Mohri D, et al. Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals. Cancer Res. 2013 Apr 1;73(7):2221-2234.
    Species: Human
    Sample type: Cell Lysate (pancreatic ductal carcinoma)
  7. Diagaradjane P,Orenstein-Cardona J, Colo¤n-Casasnovas N, Deorukhkar A, Shentu S. Imaging Epidermal Growth Factor Receptor Expression In vivo: Pharmacokinetic and Biodistribution Characterization of a Bioconjugated Quantum Dot Nanoprobe. Clin Cancer Res.2008;14; 731
    Species: Human
    Sample type:
  8. Ikari A., Sato T., Watanabe R., et al. Increase in claudin-2 expression by an EGFR/MEK/ERK/c-Fos pathway in lung adenocarcinoma A549 cells. Biochim Biophys Acta. 2012 Jun;1823(6):1110-8. doi: 10.1016/j.bbamcr.2012.04.005.
    Species: Human
    Sample type: Conditioned Media (A549, human lung adenocarcinoma, RERF-LC-AI, squamous cells, IA-LM, large cells, and WA-hT, small cells)
  9. Plagg B., Marksteiner J., Kniewallner K., et al. Platelet dysfunction in hypercholesterolemia mice, two Alzheimer's disease mouse models and in human patients with Alzheimer's disease. Biogerontology. 2015 Aug;16(4):543-58. doi: 10.1007/s10522-015-9580-1.
    Species: Human
    Sample type: Platelet Releasate (Platelet Releasates)
  10. Zhang Y., Lin P., Jiang H., et al. Extensive serum biomarker analysis in patients with ST segment elevation myocardial infarction (STEMI). Cytokine. 2015 Dec;76(2):356-62. doi: 10.1016/j.cyto.2015.06.015.
    Species: Human
    Sample type: Serum (Biomarker analysis of ST Segment elevation myocardial infarction (STEMI))

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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