Protein Name & Synonyms:
Epidermal growth factor receptor (EC 184.108.40.206) (Proto-oncogene c-ErbB-1) (Receptor tyrosine-protein kinase erbB-1)
Functional Activity (Inhibition): antibody blocks EGF receptor activation, inhibits A431 cell proliferation, arrests tumor growth in vivo, affinity purification
IP: 1-2µg/500ug protein lysate
FACS: 0.5-1ug/million cells
1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free
Microsomes from A431 cells were used as the immunogen for this antibody.
Protein G purified monoclonal antibody
Human. Does not react with mouse and rat. Other species not known.
Mouse IgG2a, kappa
This antibody reacts with the extracellular domain of EGF receptor and blocks the EGF/TGFa induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGF receptor. EGFR
is type I receptor tyrosine kinase with sequence homology to ErbB2
, -3 -4 or HER2
, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGFa)
, Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.